|
Isolation, identification and phylogenetic analysis of a pathogen of Haliotis diversicolor supertexta (L.) with mass mortalities
|
Zhou Jing *   , Cai Junpeng |
| , jingzhou-2004@163.com |
|
|
Abstract: (8878 Views) |
| This study was conducted to determine a disease outbreak in 14 day old post-larvae of abalone (Haliotis diversicolor supertexta) which caused mass mortality in July 2010 in Shanwei, China. Twenty-nine bacterial strains were isolated from a sample pool of 10 diseased post-larval abalones on 2216E marine agar plates during a natural outbreak of the disease. Among them, a dominant isolate (referred to as strain 21) was found to be highly virulent to post-larvae in experimental challenge tests, with an LD50 value of 1.0 ×104 colony forming units (CFU) mL-1 on day 3. API 20NE kits and 16S rDNA sequence analysis, identified strain 21 as Oceanomonas doudoroffii. It was susceptible to 10 and moderately susceptible to 1 of the 16 antibiotics examined when antibiotic sensitivities of the bacterium were assayed. Results of this study implicated Oceanomonas doudoroffii strain 21 as a cause of mortalities in post-larval abalone from Shanwei, China. |
|
| Keywords: Haliotis diversicolor supertexta, massive death, challenge test, Oceanomonas doudoroffii, 16S rDNA sequencing |
|
|
Full-Text [PDF 108 kb]
(2629 Downloads)
|
Type of Study: Orginal research papers |
Subject:
fish disease
Received: 2014/01/27 | Accepted: 2014/01/27 | Published: 2014/01/27
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
.png)
.png)
.png)
.png)
.png)
