[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
Main Menu
Home::
Journal Information::
Articles archive::
Registration::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Webmail::
Publication Ethics::
::
Indexed & full text in

AWT IMAGE 

..
Journal's Impact Factor

 

             "If you have any questions or concerns, please contact us by email

 "ijfs.ifro(at)yahoo.com"
Journal`s Impact Factor 2023:0.985
SJR: Q3
Journal's Impact Factor ISC 2020: 0.476

 

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Abstract in

 پایگاه استنادی علوم جهان اسلام (ISC)

پایگاه اطلاعات جهاد دانشگاهی (SID) 



بانک نشریات کشور (مگیران)


https://www.stomaeduj.com/wp-content/uploads/2019/12/publons.png





scholar.google

..

..
Journal DOI

AWT IMAGE
Journal DOI

10.18869/acadpub.ijfs
 

..
:: Volume 17, Issue 4 (2018) ::
IJFS 2018, 17 Back to browse issues page
Immunization of Basa fish (Pangasius bocourti) against Ichthyophthirius multifiliis with live and sonicated trophonts
N. Uawonggul * , C. Rattanamalee , S. Daduang
Faculty of Science, Nakhon Phanom University, Nakhon Phanom, 48000, Thailand , nunthaua@npu.ac.th
Abstract:   (3521 Views)
The high density of Basa fish (Pangasius bocourti) culture leads to outbreaks of Ichthyophthirius multifiliis (Ich), also knows as white spot disease. In this research, immunization of Basa fish against Ich with live and sonicated trophonts by intraperitoneal (IP) injection was investigated. Anti‑Ich antibody titer was determined using ELISA and Western immunoblotting 21 days post immunization. The results revealed that pre-immunized fish, non‑immunized fish and fish immunized with bovine serum albumin (BSA)  at a concentration 65 µg g-1 fish did not show specific antibody against Ich. 21 days post immunization, fish immunized with live trophonts exhibited higher anti‑Ich antibody titer than fish immunized with sonicated trophonts at the same antigen concentration. Fish immunized with 65 µg trophonts protein/g fish live trophonts showed the highest titer 1:1,000 (p<0.05). The results from Western immunoblotting showed two parasite protein bands of 66 kDa and <14 kDa, which reacted with antibodies from serum of immune fish. No fish in the non-immunized group survived. At the same concentration of antigen (65 µg g-1), fish immunized with live trophonts exhibited the highest survival rate, 63.33±5.77% (p<0.05). Therefore, these results are the Basa fish immunizing procedure will be the way to conduct immunization against Ich to prevent disease outbreaks in aquaculture.
 
Keywords: Pangasius bocourti, Ichthyophthirius multifiliis, Immunization
Full-Text [PDF 410 kb]   (2377 Downloads)    
Type of Study: Orginal research papers |
Received: 2018/09/25 | Accepted: 2018/09/25 | Published: 2018/09/25
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Uawonggul N, Rattanamalee C, Daduang S. Immunization of Basa fish (Pangasius bocourti) against Ichthyophthirius multifiliis with live and sonicated trophonts. IJFS 2018; 17 (4) :763-775
URL: http://jifro.ir/article-1-3622-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 17, Issue 4 (2018) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.06 seconds with 44 queries by YEKTAWEB 4645