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IJFS 2018, 17 Back to browse issues page
Immunization of Basa fish (Pangasius bocourti) against Ichthyophthirius multifiliis with live and sonicated trophonts
N. Uawonggul *, C. Rattanamalee, S. Daduang
Faculty of Science, Nakhon Phanom University, Nakhon Phanom, 48000, Thailand , nunthaua@npu.ac.th
Abstract:   (1512 Views)
The high density of Basa fish (Pangasius bocourti) culture leads to outbreaks of Ichthyophthirius multifiliis (Ich), also knows as white spot disease. In this research, immunization of Basa fish against Ich with live and sonicated trophonts by intraperitoneal (IP) injection was investigated. Anti‑Ich antibody titer was determined using ELISA and Western immunoblotting 21 days post immunization. The results revealed that pre-immunized fish, non‑immunized fish and fish immunized with bovine serum albumin (BSA)  at a concentration 65 µg g-1 fish did not show specific antibody against Ich. 21 days post immunization, fish immunized with live trophonts exhibited higher anti‑Ich antibody titer than fish immunized with sonicated trophonts at the same antigen concentration. Fish immunized with 65 µg trophonts protein/g fish live trophonts showed the highest titer 1:1,000 (p<0.05). The results from Western immunoblotting showed two parasite protein bands of 66 kDa and <14 kDa, which reacted with antibodies from serum of immune fish. No fish in the non-immunized group survived. At the same concentration of antigen (65 µg g-1), fish immunized with live trophonts exhibited the highest survival rate, 63.33±5.77% (p<0.05). Therefore, these results are the Basa fish immunizing procedure will be the way to conduct immunization against Ich to prevent disease outbreaks in aquaculture.
 
Keywords: Pangasius bocourti, Ichthyophthirius multifiliis, Immunization
Full-Text [PDF 410 kb]   (718 Downloads)    
Type of Study: Orginal research papers |
Received: 2018/09/25 | Accepted: 2018/09/25 | Published: 2018/09/25
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Uawonggul N, Rattanamalee C, Daduang S. Immunization of Basa fish (Pangasius bocourti) against Ichthyophthirius multifiliis with live and sonicated trophonts. IJFS. 2018; 17 (4) :763-775
URL: http://jifro.ir/article-1-3622-en.html


Volume 17, Issue 4 (2018) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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