[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 19, Issue 3 (2020) ::
IJFS 2020, 19 Back to browse issues page
The pretreatment potential effects of omy-miR-731 Antagomir in brain tissue of rainbow trout Oncorhynchus mykiss infected with viral hemorrhagic septicemia virus
N. Zamannejad *, M.R. Bigdeli, A.A. Motallebi, H. Kohram, A. Haghighi Khiabanian Asl
ph. D student Shahid Beheshti University , zamannejadnooshin@gmail.com
Abstract:   (405 Views)
Among the viral diseases in trout farming, viral hemorrhagic septicemia (VHS) could be mentioned as the most devastating worldwide viral infection. In the present investigation, the influence of the omy-miR-731 Antagomir pretreatment on VHS in rainbow trout was examined. Trout (20±1) g were examined in four main groups subdivided into three subgroups, including a negative control without any injection, a vehicle that received saline, a positive control that was infected by VHSV, and an experimental group that received omy-miR-731 Antagomir by injection, then they were challenged by VHSV. The survival rate, average weight gain, histopathology, Immunohistochemistry and Blood-brain barrier impermeability were assessed in each group. The survival rate and average weight gain in the positive control group was significantly decreased in comparison with other groups. Remarkable histopathological changes and virus detection by immunohistochemistry test were observed in the positive control, whereas no sign of the virus was observed in the vehicle and the negative control groups. The histologic changes in the experimental group were significantly less than the positive control group. Also, the Blood-brain barrier impermeability was significantly decreased in the other groups compared to the positive control group. Although more studies are needed to verify the exact mechanisms of pretreatment potential effects of omy-miR-731 Antagomir, the present results proved that pretreatment potential of omy-miR-731 Antagomir can protect the brain tissue against signs of VHS and preferably would be a new approach against VHSV infection in rainbow trout.
Keywords: Viral hemorrhagic septicemia, omy-miR-731 Antagomir, Blood-brain barrier, Flurescent isothiocyanate, Neurohistopathology, Immunohistochemistry.
Full-Text [PDF 1643 kb]   (172 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2018/08/31 | Accepted: 2018/12/16 | Published: 2020/05/1
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zamannejad N, Bigdeli M, Motallebi A, Kohram H, Haghighi Khiabanian Asl A. The pretreatment potential effects of omy-miR-731 Antagomir in brain tissue of rainbow trout Oncorhynchus mykiss infected with viral hemorrhagic septicemia virus. IJFS. 2020; 19 (3) :1615-1637
URL: http://jifro.ir/article-1-3584-en.html


Volume 19, Issue 3 (2020) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 4212