[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 19, Issue 6 (2020) ::
IJFS 2020, 19 Back to browse issues page
Research Article: Molecular diagnosis of pathogenic Aeromonas hydrophila in Cyprinus carpio ulcers
I.R. Ibrahim, S.M.A. Abdullah, A.Y. Karim *
Biology Department, College of Science, Salahaddin University-Erbil, Erbil, Iraq. , Abdulkarimy.karim@ymail.com
Abstract:   (616 Views)
A total of 150 carp fish were collected from Grdarasha Farm (8 kilometers from Erbil-Iraq) and examined for the presence of pathogenic Aeromonas hydrophila on their skin ulcers, this bacterium secretes a toxin protein called aerolysin which is a virulence factor and has a role in diarrhoeal diseases, deep wound infections and hemorrhagic septicemia. The present study aimed to determine the presence of A. hydrophila in common carp (Cyprinus carpio). For this purpose samples were collected from fish ulcer by swap and incubated at 37°C in blood agar to determine the shape and morphology of colonies, and then the colonies were examined by gram stain test for microscopic examination, finally molecular diagnosis was conducted for aerolysin gene with primer design and PCR. According to the diagnosis methods results were different; PCR diagnosis confirmed that only 68 skin lesions were caused by A. hydrophila, no visceral lesion was caused by A. hydrophila and this bacterium hardly ever seen in the skin surrounding mouth as only 2 lesions surrounding mouth had A. hydrophila in PCR diagnosis.
 
Keywords: Aerolysin, Aeromonas hydrophila, Cyprinus carpio, Polymerase chain reaction
Full-Text [PDF 260 kb]   (294 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2018/06/13 | Accepted: 2019/05/18 | Published: 2020/11/20
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ibrahim I, Abdullah S, Karim A. Research Article: Molecular diagnosis of pathogenic Aeromonas hydrophila in Cyprinus carpio ulcers. IJFS. 2020; 19 (6) :3160-3169
URL: http://jifro.ir/article-1-3472-en.html


Volume 19, Issue 6 (2020) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4319