[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 19, Issue 2 (2020) ::
IJFS 2020, 19 Back to browse issues page
Application of Bacillus amyloliquefaciens as probiotic for Litopenaeus vannamei (Boone, 1931) cultivated in a biofloc system
F. Llario *, L.A. Romano, M. Rodilla, M.T. Sebastiá-Frasquet, L.H. Poersch
Insitut d’Investigació per a la Gestió Integrada de Zones Costaners (IGIC). Universitat Politècnica de València (UPV). , ferllase@upv.es
Abstract:   (645 Views)
Probiotics can improve growth, survival and resistance to pathogenic organisms of the cultivated species in aquaculture systems with water recirculation. However, their possible benefits on biofloc systems have been less studied. In this study, the benefits of Bacillus amyloliquefaciens bacterium, on a biofloc culture of Litopenaeus vannamei were evaluated. B. amyloliquefaciens was applied as dissolved in water. To our knowledge, no previous assays on biofloc systems have been published, and on recirculation systems it has only been tested mixed with feed. The objective of the present study was to evaluate the effect of B. amyloliquefaciens on water quality, growth parameters and the immune system of shrimp. Three concentrations of probiotic were tested in triplicate (9.48 × 104, 1.90 × 105, and 3.79 × 105 cfu ml-1) and were compared with the control (without probiotics). Water quality parameters such as nutrients and suspended solids were monitored. In L. vannamei, growth, survival and their immune system parameters (total protein concentration, cell number with apoptosis and percentage of granular and hyaline hemocytes) were studied. The results showed that the application of B. amyloliquefaciens did not produce significant differences in water quality or shrimp growth. However, it showed significant improvements in the immune system. As compared with the control treatment, an increase in the total protein concentration and granular hemocytes, and a decrease in the cell number with apoptosis in the hempolymph were observed. Thus, we can conclude that B. amyloliquefaciens provides greater resistance to shrimp against the attack of pathogens in biofloc systems.
Keywords: Growth parameters, Immunological parameters, Water quality, White shrimp
Full-Text [PDF 581 kb]   (438 Downloads)    
Type of Study: Orginal research papers | Subject: aquaculture
Received: 2018/01/31 | Accepted: 2018/07/30 | Published: 2020/03/20
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Llario F, Romano L, Rodilla M, Sebastiá-Frasquet M, Poersch L. Application of Bacillus amyloliquefaciens as probiotic for Litopenaeus vannamei (Boone, 1931) cultivated in a biofloc system. IJFS. 2020; 19 (2) :904-920
URL: http://jifro.ir/article-1-3294-en.html


Volume 19, Issue 2 (2020) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.04 seconds with 32 queries by YEKTAWEB 4212