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IJFS 2007, 6 Back to browse issues page
Effects of cortisol on tesicular apoptosis in goldfish (Carassius auratus)
M. Bahmani *, C.V. Andreu-Vieyra, H.R. Habibi
, mahmoudbahmani@yahoo.com
Abstract:   (1612 Views)
Apoptosis is a programmed cell death that has been attributed to the action of stress hormones, such as cortisol. We investigated the in vitro effect of cortisol in goldfish (Carassius auratus) mature (GSI: 2.75-4.65) or immature (GSI: 1.94-2.4) testes. Testes were taken out and transferred into culture medium (M 199), cut into 20 mm thick slices and supplemented with fetal bovine serum (FBS), added different doses of cortisol (0, 50, 100, 200, 400, 750 and 800 ng/ml) and incubated for 24h, 48h or 72h at 18°C. Samples were rinsed and homogenized with phosphate buffered saline (PBS) after incubation period. Duplicates or triplicates processed for caspase-3 activity. The results showed a significant increase in caspase-3 activity in immature testes after 72h treatment with 750ng/ml of cortisol (P<0.05), and also at 400ng/ml (P<0.05). In contrast, no significant differences were observed between non-treated and cortisol-treated samples from mature goldfish testes. In this study we have demonstrated, for the first time that the stress hormone cortisol induces apoptosis in the goldfish testis in a stage-specific manner (immature testis).
Keywords: Apoptosis, Caspase 3, Cortisol, Goldfish, Stress, Testis
Full-Text [PDF 2554 kb]   (287 Downloads)    
Type of Study: Orginal research papers | Subject: Biology & physiology
Received: 2017/12/9 | Accepted: 2017/12/9 | Published: 2017/12/9
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Bahmani M, Andreu-Vieyra C, Habibi H. Effects of cortisol on tesicular apoptosis in goldfish (Carassius auratus). IJFS. 2007; 6 (2) :1-14
URL: http://jifro.ir/article-1-3099-en.html

Volume 6, Issue 2 (2007) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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