The purpose of this study was to evaluate the effects of aluminum on the tissue enzymes of gills, liver and muscles in common carp. In this study, the effects of sub lethal levels of aluminum on metabolic enzymes, Aspartate aminotransferase, Alanine aminotransferase and alkaline phosphatase (AST, ALT and ALP) in organs including gills, liver and muscles of common carp (Cyprinus carpio) were evaluated after 24, 48, 72 and 96 h exposure under laboratory conditions. Tissue extracts were prepared from each group. Enzymes were measured using commercial kits and auto-analyzer Selectra-PRO. According to statistical data analysis, it was found that the activity of this enzyme in tissues increased significantly compared to that in the control group. AST enzymes in the liver and muscle increased significantly but were reduced at 96 h. ALP levels in muscle tissue significantly increased at 72 h but were reduced at 96 h. The alterations in the activities of these enzymes indicated that the presence of aluminum interfered with transamination and metabolic process in tissues. As a biomarker in various organs, it can also be useful for toxicology