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:: دوره 20، شماره 5 - ( 1400 ) ::
جلد 20 شماره 5 صفحات 1408-1387 برگشت به فهرست نسخه ها
Research Article: Comparative effect of different vaccines on immune-related gene expressions of rainbow trout (Oncorhynchus mykiss) and experimentally infected with Streptococcus iniae and Lactococus garvie
چکیده:   (329 مشاهده)
To understand how different vaccines, i.e., bivalent formalin-inactivated vaccine (Lactococus garvie and Streptococcus iniae), could affect the immune-related gene expressions, the TNF-alpha, IL-1β and IL-8 genes of head kidney was measured in Oncorhynchus mykiss. To address this, 630 juvenile fish weighing 26±3 g were initially (day 0) injected by three different treatments, including control group (normal saline only), AquaVacTM Garvetil (Merck) and Iranian vaccine (Jehad-e-Daneshagahi) and followed by bath immersion into the same vaccine on day 30. The head kidney was withdrawn to measure immune-related gene expressions at the beginning of experiment (day 0), and following 14, 30, 45 and 60 days of post-vaccination. In the next step, control and both vaccinated groups were also subjected to either L. garvie or S. iniae challenge. Similar samples were taken immediately following bacteria injection (time= 0) and 12, 48, 72 h, and following 7 and 10 days of challenged test. The real-time PCR indicated up-regulation of all cytokine genes following vaccination at day 14 as compared to initial day (p<0.05). In pre-challenged experiment, the higher induction in the level of those genes in the head kidney was related to AquaVac vaccine (p<0.05). Injection by S. iniae and L. garviae induced the level of immune-related gene expression in the head kidney within the first few days with higher intensity in case of unvaccinated control group. Although the AquaVac produces higher up-regulation of inflammatory gene expression rather than Iranian vaccine, the immunostimulatory effects of both vaccines is a time-restricted.
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نوع مطالعه: پژوهشي | موضوع مقاله: fish disease
دریافت: 1397/12/21 | پذیرش: 1400/6/28 | انتشار: 1400/6/28
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Khaj H, Mesbah M, Tabandeh M, Mohammadian T, Dadar M. Research Article: Comparative effect of different vaccines on immune-related gene expressions of rainbow trout (Oncorhynchus mykiss) and experimentally infected with Streptococcus iniae and Lactococus garvie. IJFS. 2021; 20 (5) :1387-1408
URL: http://jifro.ir/article-1-3838-fa.html

Research Article: Comparative effect of different vaccines on immune-related gene expressions of rainbow trout (Oncorhynchus mykiss) and experimentally infected with Streptococcus iniae and Lactococus garvie. مجله علوم شیلاتی ایران. 1400; 20 (5) :1408-1387

URL: http://jifro.ir/article-1-3838-fa.html



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دوره 20، شماره 5 - ( 1400 ) برگشت به فهرست نسخه ها
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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