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Research Article: Comparative effect of different vaccines on immune-related gene expressions of rainbow trout (Oncorhynchus mykiss) and experimentally infected with Streptococcus iniae and Lactococus garvie
H. Khaj *, M. Mesbah, M.R. Tabandeh, T. Mohammadian, M. Dadar
, Hosseinkhaj@yahoo.com
Abstract:   (117 Views)
To understand how different vaccines, i.e., bivalent formalin-inactivated vaccine (Lactococus garvie and Streptococcus iniae), could affect the immune-related gene expressions, the TNF-alpha, IL-1β and IL-8 genes of head kidney was measured in Oncorhynchus mykiss. To address this, 630 juvenile fish weighing 26±3 g were initially (day 0) injected by three different treatments, including control group (normal saline only), AquaVacTM Garvetil (Merck) and Iranian vaccine (Jehad-e-Daneshagahi) and followed by bath immersion into the same vaccine on day 30. The head kidney was withdrawn to measure immune-related gene expressions at the beginning of experiment (day 0), and following 14, 30, 45 and 60 days of post-vaccination. In the next step, control and both vaccinated groups were also subjected to either L. garvie or S. iniae challenge. Similar samples were taken immediately following bacteria injection (time= 0) and 12, 48, 72 h, and following 7 and 10 days of challenged test. The real-time PCR indicated up-regulation of all cytokine genes following vaccination at day 14 as compared to initial day (p<0.05). In pre-challenged experiment, the higher induction in the level of those genes in the head kidney was related to AquaVac vaccine (p<0.05). Injection by S. iniae and L. garviae induced the level of immune-related gene expression in the head kidney within the first few days with higher intensity in case of unvaccinated control group. Although the AquaVac produces higher up-regulation of inflammatory gene expression rather than Iranian vaccine, the immunostimulatory effects of both vaccines is a time-restricted.
Keywords: Cytokine genes, Pro-inflammatory genes, Bacterial infection, Passive vaccination, Oncorhynchus mykiss
Full-Text [PDF 886 kb]   (69 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2019/03/12 | Accepted: 2021/09/19 | Published: 2021/09/19
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Khaj H, Mesbah M, Tabandeh M, Mohammadian T, Dadar M. Research Article: Comparative effect of different vaccines on immune-related gene expressions of rainbow trout (Oncorhynchus mykiss) and experimentally infected with Streptococcus iniae and Lactococus garvie. IJFS. 2021; 20 (5) :1387-1408
URL: http://jifro.ir/article-1-3838-en.html


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Volume 20, Issue 5 (2021) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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