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 پایگاه استنادی علوم جهان اسلام (ISC)

پایگاه اطلاعات جهاد دانشگاهی (SID) 

بانک نشریات کشور (مگیران)




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:: Volume 14, Issue 2 (2015) ::
IJFS 2015, 14 Back to browse issues page
Molecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate
D Hosseini *
, hosseinida@yahoo.com
Abstract:   (6106 Views)
White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including Iran. In the present study, a pair of primers was designed according to the sequence of VP 28 gene of WSSV in the GenBank. VP28 gene from an Iranian WSSV isolate (IrVP28) was cloned, sequenced and expressed in Escherichia coli BL21(DE3) strain in order to produce VP28 protein using PET28a expression vector. The expression resulted in a protein of about 30kDa, which was purified under denaturing conditions, resulting in a highly purified final IrVP28 preparation. The obtained recombinant protein can be used in several biotechnology applications, such as production of monoclonal antibodies which could be used in development of diagnostic tools and potential oral vaccination of shrimp with vaccines consisting of VP28 proteins.
Keywords: WSSV, VP28, PET28a, Recombinant protein
Full-Text [PDF 366 kb]   (2112 Downloads)    
Type of Study: Orginal research papers | Subject: Biotechnology
Received: 2013/02/9 | Accepted: 2014/11/29 | Published: 2015/06/28
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Hosseini D. Molecular cloning and recombinant expression of the VP28 (wsv421 gene) from Iranian white spot syndrome virus isolate . IJFS 2015; 14 (2) :446-456
URL: http://jifro.ir/article-1-891-en.html

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Volume 14, Issue 2 (2015) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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