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Chitinase Isolated from Water and Soil Bacteria in Shrimp farming Ponds
M. Zarei, S. Aminzadeh *, A. Ghoroghi, A. A. Motalebi, J. Alikhajeh, M. Daliri
, aminzade@nigeb.ac.ir
Abstract:   (14366 Views)

Chitinases have received attention because of their wide applications in the medicine, biotechnology, agriculture, waste management and industrial applications such as food quality enhancer and biopesticide. Excessive use of insecticides has led to several problems related to pollution and environmental degradation. In this study, isolation and identification of native bacterial strains with chitin hydrolysis activity, took place from water and soil of shrimp culture ponds in Bushehr and Abadan. To investigate the capacity of our chitinase for using in insecticide, biochemical properties of selected chitinase obtained in this research were compared to that of produced by Bacillus cereus p-1, isolated from an insecticide tablet. In this research, three mesophilic strains containing: Serratia marcescens B4A, Citrobacter freundii B1A and Bacillus cereus B3R were isolated. Results showed a 1600 bp band corresponding to chitinase gene. The similarity between temperature and pH profile and stability of chitinase was extracted from native bacteria and ones was obtained from Bacillus cereus p-1 implied that chitinase extracted from Serratia marcescens B4A has potential application in industry.

Keywords: Chitinase, Chitin, Shrimp pond, Bacteria
Full-Text [PDF 493 kb]   (8154 Downloads)    
Type of Study: Orginal research papers | Subject: aquaculture
Received: 2012/11/16 | Published: 2012/10/15
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Zarei M, Aminzadeh S, Ghoroghi A, Motalebi A A, Alikhajeh J, Daliri M. Chitinase Isolated from Water and Soil Bacteria in Shrimp farming Ponds . IJFS 2012; 11 (4) :911-925
URL: http://jifro.ir/article-1-748-en.html

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Volume 11, Issue 4 (2012) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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