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Research Article: Phytase supplementation enhances mineral digestibility and blood indices in Catla catla fed plant-based diets
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A. Sabir1   , M.Z.H. Arsalan1 , T. Ahmed1 , S.M. Hussain *2 , S. Ali3 , H. Naz4 , Z. Akram5 , A. Naeem6 , E. Naeem6 |
1- Department of Life Sciences, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, Pakistan.
2- Fish Nutrition Laboratory, Department of Zoology, Government College University Faisalabad, Pakistan , drmakhdoomhussain@gcuf.edu.pk
3- Department of Environmental Sciences, Government College University, Faisalabad, Punjab 38000, Pakistan & Department of Biological Sciences and Technology, China Medical University, Taichung 40402, Taiwan
4- Department of Zoology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan
5- Fish Nutrition Lab, Department of Zoology, Government College University Faisalabad, Faisalabad 38000, Pakistan
6- Fish Nutrition Laboratory, Department of Zoology, Government College University Faisalabad, Pakistan |
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Abstract: (217 Views) |
| In the fish feed industry, fishmeal (FM) is highly valuable for its excellent combination of essential amino acids and fatty acids. However, due to its high cost and low availability, researchers are focusing on alternative sustainable plant-based protein sources. However, phytate, a major phosphorus-rich compound in plants, has a chelating effect that significantly reduces the absorption and bioavailability of essential nutrients, leading to nutritional imbalances. Adding phytase enzyme (PHY) in aqua feed improves the digestibility of minerals and reduces their excretion. Therefore, this study assessed the impact of PHY supplementation on hematological indices and mineral digestibility in Catla catla when fed different plant protein-based diets for FM replacement such as Moringa oleifera leaf meal (MOLM), sunflower meal (SFM), canola meal (CM), guar meal (GM). Nine test diets were formulated: a control diet without PHY, and eight experimental diets with PHY added at 250 or 500 FTU/kg. The results revealed that the addition of 500 FTU/kg of PHY to a 50% MOLM significantly enhances the digestibility of minerals like P, Mg, Al, Na, K, Cu, and Zn, when compared to control and other test levels. In terms of blood indices, this supplementation increased the values of RBCs (3.18×106mm-3), Hb (8.68 g/100 ml), PCV (30.07 %) and WBCs (8.20×103mm-3) count as well as serum liver enzymes alanine aminotransferase (0.93 U/L) and aspartate aminotransferase (3.11 U/L). The results indicated that the most effective combination was a diet (T3) containing 50% MOLM and 500 FTU kg-1 PHY, which yielded optimal hematological and mineral profiles. Conclusively, this study shows that PHY-supplemented plant-based fish diets reduce the need for mineral supplements, lowering feed costs and environmental impact. |
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| Keywords: Phytase, Catla catla, Plant-based diets, Fishmeal, Mineral digestibility, Hematological parameters |
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Full-Text [PDF 488 kb]
(92 Downloads)
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Type of Study: Orginal research papers |
Subject:
aquaculture
ePublished: 2026/02/17
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Sabir A, Arsalan M, Ahmed T, Hussain S, Ali S, Naz H, et al . Research Article: Phytase supplementation enhances mineral digestibility and blood indices in Catla catla fed plant-based diets. IJFS 2026; 25 (2) :197-214 URL: http://jifro.ir/article-1-5935-en.html
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