[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 21, Issue 4 (2022) ::
IJFS 2022, 21 Back to browse issues page
Research Article: Localization of Na+-K+-ATPase and Na+-K+-2Cl- cotransporter, and Na+-H+ exchanger in the renal system of Walton's mudskipper (Periophthalmus waltoni) using immunohistochemistry and histology methods
K. Esfandiyari, M. Babaei, H. Morovvati, A.M. Tarabi, A. Kalantari-Hesari *
Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran. , a.kalantarihesari@basu.ac.ir
Abstract:   (139 Views)
Kidneys play an important role in regulating the balance of water and ions in freshwater and seawater fish. However, complex kidney structures impair a comprehensive understanding of kidney function. In this study, in addition to the investigation of renal histology, Na+-K+-ATPase, Na+-K+-2Cl- cotransporter (NKCC), and Na+-H+ exchanger (NHE) were localized in the renal system of Walton's mudskipper (Priophthalmus waltoni). The kidney samples were fixed and they passed the preparing section and staining stages. The renal tubules were composed of proximal tubules and distal tubules, followed by collecting tubes and finally collecting ducts. The distribution of the Na+-K+-ATPase immune response varied in different sections of the nephron. The NKCC positioning was reported only in collecting tubes and collecting ducts, and proximal tubes and distal tubes did not respond to the antibody. Immunohistochemical response for NHE3 localization was detected only at the apex of epithelial cells of proximal tubules and collecting tubes. The distal tubes showed a negative reaction and the collecting ducts showed a weak response to NHE3 immunolocalization. In conclusion, Na+-K+-ATPase, NKCC, and NHE were differentially located in the renal system, suggesting that various physiological system operates in the renal system for ionic retention. This study provided valuable information to understand the ion regulation abilities of epithelial cells in various parts of P. waltoni nephrons.
Keywords: Periophthalmus, Nephrons tubules, Ionic retention, Antibody, Kidney
Full-Text [PDF 1069 kb]   (38 Downloads)    
Type of Study: Orginal research papers | Subject: Biology & physiology
Received: 2022/03/8 | Accepted: 2022/09/12 | Published: 2022/10/3
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Esfandiyari K, Babaei M, Morovvati H, Tarabi A, Kalantari-Hesari A. Research Article: Localization of Na+-K+-ATPase and Na+-K+-2Cl- cotransporter, and Na+-H+ exchanger in the renal system of Walton's mudskipper (Periophthalmus waltoni) using immunohistochemistry and histology methods. IJFS 2022; 21 (4) :1085-1096
URL: http://jifro.ir/article-1-5004-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 4 (2022) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4509