[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
Main Menu
Home::
Journal Information::
Articles archive::
Registration::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Webmail::
Publication Ethics::
::
Indexed & full text in

AWT IMAGE 

..
Journal's Impact Factor

 

             "If you have any questions or concerns, please contact us by email

 "ijfs.ifro(at)yahoo.com"
Journal`s Impact Factor 2023:0.985
SJR: Q3
Journal's Impact Factor ISC 2020: 0.476

 

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Abstract in

 پایگاه استنادی علوم جهان اسلام (ISC)

پایگاه اطلاعات جهاد دانشگاهی (SID) 



بانک نشریات کشور (مگیران)


https://www.stomaeduj.com/wp-content/uploads/2019/12/publons.png





scholar.google

..

..
Journal DOI

AWT IMAGE
Journal DOI

10.18869/acadpub.ijfs
 

..
:: Volume 21, Issue 5 (2022) ::
IJFS 2022, 21 Back to browse issues page
Research Article: Bioactive peptides from the Pacific white-leg shrimp (Litopenaeus vannamei) induce apoptosis and anticancer activities in HCT-116 colon cancer cell line
N. Azadi , E. H. Najdegerami * , M. Imani , M. Nikoo
Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran , e.gerami55@gmail.com
Abstract:   (910 Views)
In this study, the effects of white-leg shrimp hydrolysate protein extracted in a progressive rise in temperature (40-60°C) (Gradual) and without centrifugation (Mix) on the colon cancer cells were determined. Both bioactive peptides were prepared using enzymatic hydrolysis with alcalase. The cytotoxic effect on HCT-116 cell line was evaluated using the Neutral red and MTT assay. In-vitro, antioxidant activity was performed using DPPH, TAC, and nitric oxide assays. Apoptosis with acridine orange/etho bromide and redox changes was evaluated in the cell lines.The results of toxicity assays showed that the survival rate of the cells were decreased by increasing the concentrations of the peptides (0.3, 0.6, 1.2, and 2.4 mg/mL). Based on the results of antioxidant activity (TAC, DPPH, No), the Gradual peptides had significantly higher antioxidant activity than Mix peptides (p<0.05). In addition, the Gradual peptide increased the concentration of nitric oxide compared to the control and Mix groups (p<0.05). Results also showed that the Gradual peptides reduced the secretion of catalase and GSH enzymes in the cancer cells (p<0.05). Both types of peptides increased apoptosis in the cancerous cell line and a higher value was observed in bioactive peptides treatments The results of the present study showed that the Pacific white shrimp hydrolysates protein obtained in a progressive rise in temperature showed anti-cancer activities against colon HCT-116cancer cell line.
Keywords: Peptides, Processing by-products, Colon cancer, Anticancer activity, Litopenaeus vannamei
Full-Text [PDF 369 kb]   (469 Downloads)    
Type of Study: Orginal research papers | Subject: Biology & physiology
Received: 2021/06/2 | Accepted: 2022/05/29 | Published: 2022/12/18
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Azadi N, H. Najdegerami E, Imani M, Nikoo M. Research Article: Bioactive peptides from the Pacific white-leg shrimp (Litopenaeus vannamei) induce apoptosis and anticancer activities in HCT-116 colon cancer cell line. IJFS 2022; 21 (5) :1180-1191
URL: http://jifro.ir/article-1-4743-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 5 (2022) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.05 seconds with 45 queries by YEKTAWEB 4645