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Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus
F. Motamedi Sedeh *, M. Afsharnasab, M. Heidarieh, S. K. Shafaee, S. Rajabifar, A. Dashtiannasab, M. H. Razavi
, fmotamedi@nrcam.org
Abstract:   (10061 Views)

  White Spot Virus (WSV) is currently the most serious viral pathogen of shrimp worldwide it causes mortality up to 100% within 7-10 days in commercial shrimp farms. Infected Indian white shrimp Fenneropenaeus indicus samples were collected from Guatr shrimp site in Sistan and Baluchestan province in south of Iran and WSV infection was confirmed by Nested PCR. WSV was isolated from infected shrimp samples by centrifugation and filtration and multiplied in crayfish by intramuscular inoculation, the isolated virus was called WSV/IRN/1/2010. In order to determine the dilution resulting in 90-100% mortality in Penaeus semiculcatus, diluted virus stock in steps from 100 till 105 times in sterile PBS was injected intramuscularly to 14 shrimps in each group. Also the virus stock was diluted in steps from 1/2 till 1/32 times in sterile PBS and injected intramuscularly in Astacus leptodactylus crayfish. Therefore the LD50 of live virus stock in Astacus leptodactylus and Penaeus semiculcatus crayfish were calculated by the Karber method 10 3.29 /ml and 10 5.35 /ml, respectively.

Keywords: White Spot Virus, Titration, Karber Formula, Astacus leptodactylus, Penaeus semisulcatus
Full-Text [PDF 483 kb]   (4100 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2012/03/17 | Accepted: 2015/09/13 | Published: 2015/09/13
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Motamedi Sedeh F, Afsharnasab M, Heidarieh M, Shafaee S K, Rajabifar S, Dashtiannasab A et al . Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus. IJFS. 2012; 11 (1) :145-155
URL: http://jifro.ir/article-1-450-en.html

Volume 11, Issue 1 (2012) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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