[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
Main Menu
Home::
Journal Information::
Articles archive::
Registration::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Webmail::
Publication Ethics::
::
Indexed & full text in

AWT IMAGE 

..
Journal's Impact Factor

 

             "If you have any questions or concerns, please contact us by email

 "ijfs.ifro(at)yahoo.com"
Journal`s Impact Factor 2023:0.985
SJR: Q3
Journal's Impact Factor ISC 2020: 0.476

 

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Abstract in

 پایگاه استنادی علوم جهان اسلام (ISC)

پایگاه اطلاعات جهاد دانشگاهی (SID) 



بانک نشریات کشور (مگیران)


https://www.stomaeduj.com/wp-content/uploads/2019/12/publons.png





scholar.google

..

..
Journal DOI

AWT IMAGE
Journal DOI

10.18869/acadpub.ijfs
 

..
:: Volume 21, Issue 6 (2022) ::
IJFS 2022, 21 Back to browse issues page
Research Article: Effect of enzyme type, enzyme to substrate ratio, temperature and hydrolysis time on the yield and antioxidant effect of Saurida tumbil protein hydrolysate
S. Bahram * , M. Khezri , S.R. Javadian
Islamic Azad University, Department of Fisheries, Qaemshahr Branch, Qaemshahr, Iran. , bahramsomi@gmail.com
Abstract:   (918 Views)
It has become a hot topic to make optimal use of fisheries products and make them value added as well as find natural food substances which can effectively inhibit oxidative stress and improve human health. Given this, Saurida tumbil muscle was hydrolyzed by Alcalase and Papain at two concentrations of 2 and 4%, and at two hydrolysis times of 90 and 180 min and their antioxidant properties were compared. Antioxidant activity of the protein hydrolysates were investigated by ferrous chelating and ferric reducing, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl free radical scavenging activity tests. Samples hydrolyzed by Papain showed higher protein recovery than those hydrolyzed by Alcalase (59.9% versus 20.06%). The protein hydrolysate prepared by Papain enzyme showed significantly higher activity in removal of DPPH (IC50=2.44 mg/mL) and hydroxyl (IC50=3.93 mg/mL) free radicals than those prepared by Alcalase enzyme (DPPH IC50=2.80 mg/mL, hydroxyl IC50=8.59 mg/mL). ABTS radical scavenging activity did not show any significant difference between hydrolyzed samples with Papain or Alcalase enzymes. Hydrolyzed samples with Alcalase enzyme showed higher ferrous chelating activity (IC50=5.33 mg/mL) and ferric reducing activity (optical absorption=0.13±00 at 700 nm) than those hydrolysate prepared by Papain enzyme. Generally, the type of enzyme, its concentration and the time of hydrolysis affected the yield and antioxidant properties of Saurida tumbil muscle protein hydrolysates and hydrolysis with papain at concentration of 4% for 180 min provided the best properties.
Keywords: Alcalase, Papain, Fish Protein Hydrolysate, Saurida tumbil, Antioxidative peptides
Full-Text [PDF 489 kb]   (672 Downloads)    
Type of Study: Orginal research papers | Subject: Fish processing
Received: 2020/05/1 | Accepted: 2020/12/12 | Published: 2023/02/22
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bahram S, Khezri M, Javadian S. Research Article: Effect of enzyme type, enzyme to substrate ratio, temperature and hydrolysis time on the yield and antioxidant effect of Saurida tumbil protein hydrolysate. IJFS 2022; 21 (6) :1397-1416
URL: http://jifro.ir/article-1-4311-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 6 (2022) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.06 seconds with 45 queries by YEKTAWEB 4645