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 پایگاه استنادی علوم جهان اسلام (ISC)

پایگاه اطلاعات جهاد دانشگاهی (SID) 



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:: Volume 19, Issue 6 (2020) ::
IJFS 2020, 19 Back to browse issues page
Research Article: Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila
S.M.A. Ebne al-Torab , H. Ahari * , S. Kakoolaki
Agricultural Sciences and Food Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran , Assoc.Prof.
Abstract:   (2557 Views)
Our study was focused on characterization of dominant Bacillus bacteria isolated from the digestive tract of Oncorhynchus mykiss. After 8 weeks, the fish fed with probiotic-free diet were dissected under strictly controlled conditions. Fish intestines were eviscerated and its content diluted to 10−4 using Ringer solution. Two isolated bacteria were Gram-positive, catalase-positive, and oxidase-negative, based on the biochemical tests. A mixed of the two strains was the most efficient isolates determined in O. mykiss. Molecular characterization using PCR-Ribotyping was carried out using sequencing the 16S rRNA gene of both strains, which isolated two bacteria, Bacillus subtilis Ahari.H1 and B. licheniformis Ahari. H2. The isolated bacteria could tolerate 0.3% bile salt and non-hemolytic in nature. They also showed resistance to pH at 2.5 and 4 for acid test and 2.3 and 7 for gastric juice, pepsin and trypsin. It is concluded that the efficiency of the isolated probiotics can be candidates as probiotic in fish culture. It was unveiled that these strains can successfully diminish Aeromonas hydrophila pathogenicity in rainbow trout.
 
Keywords: Rainbow trout, Probiotic, Bacillus subtilis Ahari.H1, Bacillus licheniformis Ahari.H2, PCR
Full-Text [PDF 874 kb]   (1873 Downloads)    
Type of Study: Orginal research papers | Subject: aquaculture
Received: 2019/09/3 | Accepted: 2020/06/2 | Published: 2020/11/20
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Ebne al-Torab S, Ahari H, Kakoolaki S. Research Article: Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila. IJFS 2020; 19 (6) :2824-2845
URL: http://jifro.ir/article-1-4043-en.html


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Volume 19, Issue 6 (2020) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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