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Research Article: Chlorella growth factor extraction and its effect on gene expression of types
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S. Abdolbaghian   , Sh. Jamili * , A. Manayi , A. Mashinchian Moradi |
| Iranian Fisheries Research Organization, Tehran, Iran , shahlajamili45@yahoo.com |
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Abstract: (2566 Views) |
| Skin protects the body from outer factors as a barrier, and contains an important cosmetic role. Skin aging is related to collagen degradation and increasing multiple enzymes, including matrix metalloproteinase (MMPS), which degrade collagen. Chlorella vulgaris is a marine alga that exhibits anti-aging activity. The beneficial effects of Chlorella on skin make it a proper ingredient to be used in anti-aging products. In this study, the effect of Chlorella growth factor (CGF) on types І and ІІІ collagen in human fibroblast cell line Hu02 was investigated. To find an effective extraction method in the present study, CGF was extracted using hot water extraction, enzymatic hydrolysis, and ultrasonication plus enzymatic hydrolysis, and the yields were compared. The yield of ultrasonication plus enzymatic hydrolysis method (CGF-3) had the strongest absorbance at 260 nm and highest solid recovery, compared to the other two methods. Using quantitative PCR, it was confirmed that CGF increased collagens І and ІІІ expression in skin fibroblast cells. This finding indicated that CGF induced collagen synthesis in Hu02 cells. Gene expression of types І and ІІІ collagen were elevated to 3.144-fold and 1.14-fold in the CGF-3 group, respectively, comparing to the control group. |
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| Keywords: Chlorella growth factor, Collagen synthesis, Anti-aging, Human skin |
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Full-Text [PDF 567 kb]
(2585 Downloads)
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Type of Study: Orginal research papers |
Subject:
Biotechnology
Received: 2019/05/9 | Accepted: 2019/06/30 | Published: 2021/11/19
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