[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
Main Menu
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Publication Ethics::
Indexed & full text in


Journal's Impact Factor


             "If you have any questions or concerns, please contact us by email

Journal`s Impact Factor 2023:0.985
Journal's Impact Factor ISC 2020: 0.476


Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
Abstract in

 پایگاه استنادی علوم جهان اسلام (ISC)

پایگاه اطلاعات جهاد دانشگاهی (SID) 

بانک نشریات کشور (مگیران)




Journal DOI

Journal DOI


:: Volume 18, Issue 1 (2019) ::
IJFS 2019, 18 Back to browse issues page
Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay
H.R. Alizadeh Sabet , M.R. Kalbassi * , M. Pourkazemi , M. Sadeghizadeh
Department of Aquaculture, Tarbiat Modares University, Faculty of Marine Sciences, Noor, Iran , kalbassi_m@modares.ac.ir
Abstract:   (3469 Views)
Caspian trout (Salmo trutta caspius Kessler, 1877) as endemic migratory fish, possessing great ecological economic and biodiversity value. Among the habitat drivers, temperature related effects on the genome were considered in this research. During the embryonic developmental stages, genomic alterations of single cells were assessed by Comet assay. After artificial propagation of wild broodstocks, the batches of eggs, were transferred to incubators with constant temperatures, REDD water recycling, 3 stages water filtration, UV-C application on effluent of each incubator and DO–pH–temperature control. Samples were collected from normal (8°C), cold (4°C) and warm (12°C) treatments on eyed egg, alevin and larval stages. Single Cell Gel Electrophoresis were applied on samples for evaluation of Comet Tail length (TL), DNA in tail (DT) and Tail Moment (TM) indices as DNA damage markers. Comparison between mean values of Comet TL index indicated 85.19 ±9.2, 9.88 ±2.3 and 256.54±14.6 for 4, 8 and 12°C treatments, respectively. DT were 18.07±6.3, 9.72±1.9 and 31.47±2.8 and then TM were measured as 15.27± 4.1, 1.21±0.2 and 95.25±8.6 for 4, 8, and 12°C treatments, respectively. Significant difference between thermal treatments (p<0.05) observed. Warm treatments demonstrated greater DNA damage in compare with others. TM explained better information in comparison with TL and DT in all analyzed samples. In conclusion, temperatures out of the range of the optimal conditions could significantly affect Caspian trout genome which could be the subject of management considerations pertaining to Caspian trout stocks rehabilitation and captive rearing strategies.[PN1] 

Keywords: Caspian trout, Salmo trutta caspius, Temperature, DNA damage, Comet assay
Full-Text [PDF 652 kb]   (1732 Downloads)    
Type of Study: Orginal research papers |
Received: 2018/12/25 | Accepted: 2018/12/25 | Published: 2018/12/25
Send email to the article author

Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Alizadeh Sabet H, Kalbassi M, Pourkazemi M, Sadeghizadeh M. Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay. IJFS 2019; 18 (1) :48-59
URL: http://jifro.ir/article-1-3718-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 1 (2019) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.05 seconds with 44 queries by YEKTAWEB 4624