[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 20, Issue 6 (2021) ::
IJFS 2021, 20 Back to browse issues page
Short Communication: Antibacterial activity of oregano (Origanum vulgare) essential oil, carvacrol and thymol against fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus)
Pathirana H.N.K.S., Wimalasena S.H.M.P., De Silva B.C.J., Hossain S., Heo G.J. *
Chungbuk National University , gjheo@cbu.ac.kr
Abstract:   (176 Views)
The antibacterial activity of oregano (Origanum vulgare) essential oil (OEO), carvacrol and thymol was tested against seven Gram-negative and nine Gram-positive fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus) in Korea. Disk diffusion assay, MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) tests showed OEO, carvacrol and thymol inhibiting the growth of both Gram-negative and Gram-positive bacteria. Carvacrol and thymol showed lower MIC values than OEO against every isolate which ranged from 0.001 to 0.007 % (V/V) and 0.001 to 0.062 % (V/V), respectively. Carvacrol (MBC/MIC= 1-4) and thymol (MBC/MIC= 2-4) were bactericidal for all tested strains while OEO (MBC/MIC= 2-8) was being both bactericidal and bacteriostatic.  In every fish pathogenic bacteria, the inhibition zone diameter (IZD) increased in proportion to the oil concentration and the maximum effect was found at 100 % (V/V) concentration of OEO, carvacrol and thymol. The antibiogram pattern indicated that all the bacterial strains excluding three strains of S. iniae (S186, S530, and S131) showed resistance to one or more antibiotics. The percentage of relative inhibition zone diameter (RIZD %) exhibited high values at higher concentration of all agents. Since antibacterial activities of OEO, carvacrol and thymol were considerably effective against fish pathogenic bacteria, their use could be alternatives to treat bacterial infections in aquaculture.
Keywords: Antibacterial activity, Oregano essential oil (OEO), Carvacrol, Thymol, Fish pathogenic bacteria, Olive flounder
Full-Text [PDF 364 kb]   (95 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2017/08/24 | Accepted: 2017/10/17 | Published: 2021/11/19
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

H.N.K.S. P, S.H.M.P. W, B.C.J. D S, S. H, G.J. H. Short Communication: Antibacterial activity of oregano (Origanum vulgare) essential oil, carvacrol and thymol against fish pathogenic bacteria isolated from cultured olive flounder (Paralichthys olivaceus). IJFS. 2021; 20 (6) :1822-1834
URL: http://jifro.ir/article-1-2897-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 6 (2021) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4374