[صفحه اصلی ]   [Archive]  
:: صفحه اصلي :: درباره نشريه :: آخرين شماره :: تمام شماره‌ها :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 19، شماره 3 - ( 1399 ) ::
جلد 19 شماره 3 صفحات 1556-1572 برگشت به فهرست نسخه ها
Definite diagnosis of viral haemorrhagic septicaemia infected farmed rainbow trout and histopathologic study of acutely diseased fish in Iran
چکیده:   (782 مشاهده)
Viral haemorrhagic septicaemia (VHS) is one of the most important viral diseases in rainbow trout that has caused great losses to the Iranian rainbow trout aquaculture industry in few recent years. In the early autumn of 2015, in order to identify the possible causes of loss, farmed fish were sampled from the epicenter where massive mortalities and economic losses had occurred. Three samples were collected from relevant tissues (i.e. liver, kidney, spleen, heart, and brain) of the rainbow trout with clinical signs of VHS disease. The samples were stored in 70% ethanol, Eagle’s minimum essential medium (EMEM) and 10% formalin to perform RT-PCR, cell-culture and histopathology examinations, respectively. A virus was isolated using fish cell lines inoculated with a homogenate of the internal organs. Sequencing analysis of truncated N gene confirmed the existence of VHS virus. RT-PCR and histopathology tests indicated the suitability and simplicity of the test as a paraclinical diagnostic method for VHS virus in the region. Haemorrhage, necrosis and other changes in histological sections of the diseased fish demonstrated pathological changes identical with the typical VHS pathology in target organ of the virus (i.e. heart, spleen, liver and kidney). To our knowledge, this is one the first studies that fully focused on the identification of VHSV from farmed trout in Iran by using histopathology examinations.
متن کامل [PDF 1078 kb]   (653 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: fish disease
دریافت: 1395/10/4 | پذیرش: 1395/11/18 | انتشار: 1399/2/12
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA


XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rahnama R, Peyghan R, Seyfi Abad Shapouri M, Rezaie A, Shahbazian N. Definite diagnosis of viral haemorrhagic septicaemia infected farmed rainbow trout and histopathologic study of acutely diseased fish in Iran. IJFS. 2020; 19 (3) :1556-1572
URL: http://jifro.ir/article-1-2513-fa.html

Definite diagnosis of viral haemorrhagic septicaemia infected farmed rainbow trout and histopathologic study of acutely diseased fish in Iran. مجله علوم شیلاتی ایران. 1399; 19 (3) :1556-1572

URL: http://jifro.ir/article-1-2513-fa.html



دوره 19، شماره 3 - ( 1399 ) برگشت به فهرست نسخه ها
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4299