:: Volume 11, Issue 2 (2012) ::
IJFS 2012, 11 Back to browse issues page
Genetic characterization of Vimba vimba persa (Pallas, 1814) in Southern parts of the Caspian Sea using microsatellite markers
S. Mohamadian *, S. Rezavani Gilkolaei, S. Rouhollahi, J. Taghavi M., M. Nayerani, E. Shirzad, A. Taheri Mirghaed
Abstract:   (9344 Views)

Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 40 regions along the Iranian coastline of the Southern Caspian Sea (Anzali lagoon and Havigh River in Gilan province, BabolRoud River in Mazandaran province and GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by the Phenol-Chlorophorm method and PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 13 loci were amplified, in which 10 of them were amplified with reasonable polymorphism and 3 were monomorphism. A total of 302 alleles were identified on average 7.55. Observed and expected heterozygosity averages were 0.80 and 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p≤0.01). The estimation of Fst (p≤0.01) revealed significant population structuring and an estimation of the four population of Vimba vimba persa was identified in the Caspian Sea in which restocking of these species should be considered.

Keywords: Vimba vimba persa, Population genetic, Microsatellite, Caspian Sea, Iran
Full-Text [PDF 609 kb]   (3416 Downloads)    
Type of Study: Orginal research papers | Subject: Genetics
Received: 2012/06/10 | Published: 2012/04/15

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Volume 11, Issue 2 (2012) Back to browse issues page