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:: Volume 17, Issue 1 (1-2018) ::
IJFS 2018, 17 Back to browse issues page
Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss)
M. Raissy * , S. Hashemi , M. Roushan , M. Jafarian , H. Momtaz , M. Soltani , E. Pirali Kheirabad
, mehdi.raissy@iaushk.ac.ir
Abstract:   (662 Views)
Lactococcosis has been defined as acute septicaemia, which causes economic losses in farmed fish, especially in rainbow trout. This study was done to evaluate the effects of the essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout. A total number of 270 fishes with a mean weight of 120 g were obtained; they were randomly divided into nine groups, each with three replicates, after two weeks of adaptation. The groups were: no injection group, vaccine only group, DMSO injection group, vaccine with 50, 100, and 200 micrograms Intraperitoneal injection (IP) injection. Two, four, and six weeks after vaccination, serological and haematological parameters were evaluated. In the sixth week, 1.7×107 cfu as LD50 96 hrs of Lactococcus garvieae were IP injected and the relative survival percentage was calculated. The results indicated that N. sativa essence is effective on the leukocyte population as the highest number of leukocytes were found in fish receiving high concentration of N. sativa. The relative survival rate of the studied fish decreased with decreasing concentrations of the N. sativa essential oil concentration, with a significant difference with control groups (p<0.05). However, using S. bachtiarica was not significantly effective on the relative survival rate of fish. The results of this study indicated that N. sativa essential oil can be used as adjuvant for L. garvieae vaccine, since it resulted in increasing leukocytes and the relative survival rate although S. bachtiarica was not effective on immune parameters of the studied fish. 
Keywords: Nigella sativa, Satureja bachtiarica, Streptococcosis, Lactococcosis, Adjuvant.
Full-Text [PDF 348 kb]   (215 Downloads)    
Type of Study: Orginal research papers |
Received: 2018/01/23 | Accepted: 2018/01/23 | Published: 2018/01/23
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Raissy M, Hashemi S, Roushan M, Jafarian M, Momtaz H, Soltani M et al . Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss). IJFS. 2018; 17 (1) :95-106
URL: http://jifro.ir/article-1-3271-en.html


Volume 17, Issue 1 (1-2018) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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