Iranian Journal of Fisheries Sciences
مجله علوم شیلاتی ایران
IJFS
Agriculture
http://jifro.ir
1
admin
1562-2916
10.18869/acadpub.ijfs
en
jalali
1396
10
1
gregorian
2018
1
1
17
1
online
1
fulltext
fa
Effects of essential oils of Satureja bachtiarica and Nigella sativa on the efficacy of lactococcosis vaccine in rainbow trout (Oncorhynchus mykiss)
پژوهشي
Orginal research papers
Lactococcosis has been defined as acute septicaemia, which causes economic losses in farmed fish, especially in rainbow trout. This study was done to evaluate the effects of the essential oils of <em>Satureja bachtiarica</em> and <em>Nigella</em> <em>sativa</em> on the efficacy of lactococcosis vaccine in rainbow trout. A total number of 270 fishes with a mean weight of 120 g were obtained; they were randomly divided into nine groups, each with three replicates, after two weeks of adaptation. The groups were: no injection group, vaccine only group, DMSO injection group, vaccine with 50, 100, and 200 micrograms <em>Intraperitoneal injection</em> (IP) injection. Two, four, and six weeks after vaccination, serological and haematological parameters were evaluated. In the sixth week, 1.7×10<sup>7</sup> cfu as LD<sub>50</sub> 96 hrs of <em>Lactococcus garvieae</em> were IP injected and the relative survival percentage was calculated. The results indicated that <em>N. sativa</em> essence is effective on the leukocyte population as the highest number of leukocytes were found in fish receiving high concentration of <em>N. sativa</em>. The relative survival rate of the studied fish decreased with decreasing concentrations of the <em>N. sativa</em> essential oil concentration, with a significant difference with control groups (<em>p</em><0.05). However, using <em>S. bachtiarica</em> was not significantly effective on the relative survival rate of fish. The results of this study indicated that <em>N. sativa</em> essential oil can be used as adjuvant for <em>L. garvieae</em> vaccine, since it resulted in increasing leukocytes and the relative survival rate although <em>S. bachtiarica</em> was not effective on immune parameters of the studied fish.
Nigella sativa, Satureja bachtiarica, Streptococcosis, Lactococcosis, Adjuvant.
95
106
http://jifro.ir/browse.php?a_code=A-10-600-150&slc_lang=fa&sid=1
M.
Raissy
mehdi.raissy@iaushk.ac.ir
100319475328460029687
100319475328460029687
Yes
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
S.
Hashemi
100319475328460029688
100319475328460029688
No
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
M.
Roushan
100319475328460029689
100319475328460029689
No
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
M.
Jafarian
100319475328460029690
100319475328460029690
No
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
H.
Momtaz
100319475328460029691
100319475328460029691
No
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
M.
Soltani
100319475328460029692
100319475328460029692
No
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
E.
Pirali Kheirabad
100319475328460029693
100319475328460029693
No
Department of Fisheries Science, College of Natural Resources and Earth Sciences, University of Shahrekord, Shahrekord, Iran.
Department of Fisheries Science, College of Natural Resources and Earth Sciences, University of Shahrekord, Shahrekord, Iran.