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:: دوره 18، شماره 4 - ( 6-1398 ) ::
جلد 18 شماره 4 صفحات 656-671 برگشت به فهرست نسخه ها
Optimization of culture conditions for growth of the Aurantiochytrium sp. shy, isolated from the Persian Gulf
چکیده:   (276 مشاهده)

In this study, a native microalgae strain with a high capability of DHA production has been isolated and purified from mangrove shores in the Persian Gulf. Morphological and molecular identification based on 18S rRNA gene showed that the isolated strain belongs to Aurantiochytrium genus. In order to optimize the growth of this strain, various environmental factors were examined, including temperature (15, 20, 25, 30 and 35°C), salinity (10, 30 and 50 g L-1) and different concentrations of nitrogen sources of yeast extract, peptone, meat extract and monosodium glutamate, each of which was tested at the concentration of 10 g L-1. The findings show that the optimal growth conditions occur at temperatures of 25-30 °C, pH equal to 6 and in the presence of meat extract as the nitrogen source. In these conditions, the cellular biomass weight, fatty acids and DHA amounts obtained were 7.34, 2.25 and 0.4 g L-1, respectively. These results imply that the new isolated strain of Aurantiochytrium sp. shy it can be suitable for lipid production.
 

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نوع مطالعه: پژوهشي | موضوع مقاله: Biotechnology
دریافت: ۱۳۹۶/۳/۲۹ | پذیرش: ۱۳۹۶/۶/۲۲
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Pahlavan Yali M, Jalili H, Noroozi M, Morady Y, Saba F. Optimization of culture conditions for growth of the Aurantiochytrium sp. shy, isolated from the Persian Gulf . IJFS. 2019; 18 (4) :656-671
URL: http://jifro.ir/article-1-2789-fa.html

Optimization of culture conditions for growth of the Aurantiochytrium sp. shy, isolated from the Persian Gulf . مجله علوم شیلاتی ایران. 1398; 18 (4) :656-671

URL: http://jifro.ir/article-1-2789-fa.html



دوره 18، شماره 4 - ( 6-1398 ) برگشت به فهرست نسخه ها
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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