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Optimization of culture conditions for growth of the Aurantiochytrium sp. shy, isolated from the Persian Gulf
M Pahlavan Yali, H Jalili , M Noroozi, Y Morady, F Saba
Life science engineering Dep. Faculty of New Sciences and Technologies-University of Tehran , hjalili@ut.ac.ir
Abstract:   (262 Views)

In this study, a native microalgae strain with a high capability of DHA production has been isolated and purified from mangrove shores in the Persian Gulf. Morphological and molecular identification based on 18S rRNA gene showed that the isolated strain belongs to Aurantiochytrium genus. In order to optimize the growth of this strain, various environmental factors were examined, including temperature (15, 20, 25, 30 and 35°C), salinity (10, 30 and 50 g L-1) and different concentrations of nitrogen sources of yeast extract, peptone, meat extract and monosodium glutamate, each of which was tested at the concentration of 10 g L-1. The findings show that the optimal growth conditions occur at temperatures of 25-30 °C, pH equal to 6 and in the presence of meat extract as the nitrogen source. In these conditions, the cellular biomass weight, fatty acids and DHA amounts obtained were 7.34, 2.25 and 0.4 g L-1, respectively. These results imply that the new isolated strain of Aurantiochytrium sp. shy it can be suitable for lipid production.
 

Keywords: Isolation, Aurantiochytrium sp., Optimization, Biomass, Microalgae, Heterotrophic.
Full-Text [PDF 397 kb]   (114 Downloads)    
Type of Study: Orginal research papers | Subject: Biotechnology
Received: 2017/06/19 | Accepted: 2017/09/13
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Pahlavan Yali M, Jalili H, Noroozi M, Morady Y, Saba F. Optimization of culture conditions for growth of the Aurantiochytrium sp. shy, isolated from the Persian Gulf . IJFS. 2019; 18 (4) :656-671
URL: http://jifro.ir/article-1-2789-en.html


Volume 18, Issue 4 (9-2019) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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