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Analysis of saline activator solution effects on sperm quality indices of Barbus sharpeyi by image J software
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M. R. Kalbassi *   , R. Lorestani , J. Ghafle Maramazi |
| , kalbassi_m@modares.ac.ir |
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Abstract: (10450 Views) |
| Regarding low fertilization rate of Barbus sharpeyi, one of the main Barbus species in south of Iran, saline activator solutions (SAS 1 and 2) were used for increasing of sperm activation and its sperm quality indices were assessed by using Image J software as a fish sperm adapted tools. SAS 1 (45mM NaCl, 5mM KCl, 30mM Tris, 188±2.3 mosmol/kg) and SAS 2 (50mM NaCl, 30mM KCl, 30mM Tris, 189±1.7 mosmol.kg) were added to a mixture of 12 male semen comparing with distilled and tap water and their effects on VCL, VSL, ALH, Linearity, %motility, spermatozoa path, % A, B and C spermatozoa type, fertilization, hatching and deformity rate were analyzed in triplicate. Results showed that SAS2 had the highest VCL (21.37±0.36 µm/s-1), VSL (11.6±0.3 µm/s-1) and motility percentage (85.75±1.56 %) among treatments. The highest A type spermatozoa percentage was recorded for SAS1 (47.16±3.15 %) and SAS2 (43.89±4.54%). The lowest C type spermatozoa percentage was recorded using SAS2 (14.24±1.56 %). Also, the highest fertilization rate was achieved from SAS2 (79.5±0.37%) and SAS1 (77.74±4%) respectively. Furthermore the lowest larval deformity rate (13.77±1.03%) was shown due to the use of SAS2. Final conclusion confirmed that SAS1 and SAS2 have positive and significant effects (P < 0.05) on extending the time length of sperms movement, VCL and VSL of B. sharpeyi which consequently resulted in better efficiency of propagation of this species in south of Iran |
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| Keywords: Sperm, Saline activator solution, Fertilization, Barbus sharpeyi, Iran |
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Full-Text [PDF 445 kb]
(4163 Downloads)
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Type of Study: Orginal research papers |
Subject:
aquaculture
Received: 2013/05/13 | Accepted: 2013/06/3 | Published: 2013/06/3
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