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Research Article: Biocompatible fish-targeted chitosan-TPP/CaP hybrid nanocarrier (Cs/Tpp-Cap) for oral DNA vaccine delivery and immune gene expression in goldfish
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چکیده: (10 مشاهده) |
| This study evaluates the potential of chitosan tripolyphosphate/calcium phosphate (Cs/Tpp-Cap) nanoparticles as carriers for oral DNA vaccine delivery in fish. In aqueous solution, positively charged Cs/Tpp-Cap nanoparticles interact electrostatically with negatively charged DNA molecules, resulting in the spontaneous self-assembly of stable nanocomplexes. This self-assembly strategy provides an innovative means of modifying anionic Cs/Tpp-Cap hybrid nanoparticles for effective oral DNA vaccine applications. The DNA vaccine was constructed by cloning the Vibrio anguillarum outer membrane porin (OMP) gene (993 bp) into the eukaryotic expression vector pcDNA3.1, generating the recombinant plasmid pVAOMP DNA. The morphology and structural characteristics of Cs/Tpp-Cap nanoparticles were characterized using scanning electron microscopy (SEM), high-resolution transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR). For encapsulation, equal volumes of heated Cs/Tpp-Cap and pVAOMP DNA solutions were combined, achieving a DNA binding efficiency of 85%. Gene distribution and expression analyses confirmed the successful incorporation of the VAOMP gene and revealed the upregulation of immune-related genes in vaccinated fish. These findings demonstrate that Cs/Tpp-Cap nanoparticles can function as an efficient, biocompatible, and non-viral delivery system for oral DNA vaccination in aquaculture species, providing a promising platform for disease prevention in fish farming. |
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متن کامل [PDF 583 kb]
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نوع مطالعه: پژوهشي |
موضوع مقاله:
Biotechnology
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