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Research Article: Mitochondrial Cyt b and CR differentiation and in silico RFLP markers for Bangladeshi and Vietnam-originated Climbing Perch (Anabas testudineus)
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Md.A. Shahariar1   , S. Aditya1 , Md.M. Hassan1 , T. Saulin1 , A. Arif2 , S.R. Deo3 , T. Sharmin1 , S. Nahar4 , M.S. Alam *5 |
1- Department of Genetics and Fish Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
2- Grameen Jano Unnayan Sangstha (GJUS), Charnoabad, Bhola, Bangladesh
3- Upazila Fisheries Office, Birol, Dinajpur, Bangladesh
4- Researcher V, Department of Laboratory Medicine, UAB Heersink School of Medicine, Birmingham, Alabama, United States of America
5- Department of Genetics and Fish Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh , msalambd@gau.edu.bd |
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Abstract: (349 Views) |
| This study employed mitochondrial Cytochrome b (Cyt b) and Control Region (CR) markers to investigate the genetic diversity and population structure of Bangladeshi and Vietnam-origin climbing perch (Anabas testudineus) populations across Bangladesh. A total of 320 individuals were collected from 16 sampling sites representing all eight administrative divisions of Bangladesh. Following quality trimming, 818 bp of Cyt b and 705 bp of CR sequences were analyzed, revealing 9 and 28 haplotypes, respectively. Analysis of molecular variance (AMOVA) indicated that the majority of genetic variation was attributable to differences among populations (Cyt b: 95.6%; CR: 89.5%). Additionally, substantial genetic differentiation was observed between Bangladeshi and Vietnam-origin groups (Cyt b: 67.5%; CR: 73.0%). Pairwise Fst values (Cyt b: 0.815; CR: 0.804) confirmed strong differentiation between Bangladeshi and Vietnam-originated groups. Bayesian phylogeny and haplotype network analyses separated Bangladeshi and Vietnam-origin stocks into distinct clades. Shared haplotypes in Dhaka and Chattogram indicated admixture with Vietnam-originated stocks. Several unique haplotypes were identified in both Bangladeshi and Vietnam-originated populations, which may serve as markers for stock identification and broodstock management. In silico RFLP analysis identified seven enzymes (AciI, HaeIII, HhaI, MboI, MspI, RsaI, and StuI) for Cyt b sequences as cost-effective, practical tools for stock identification, while no diagnostic enzymes were found for CR. These findings provide a genetic baseline for conservation and selective breeding, and highlight the urgent need to protect Bangladeshi A. testudineus from admixture with exotic strains. |
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| Keywords: Bangladesh, Climbing perch, Control Region, Cyt b, mtDNA, Population structure, RFLP markers |
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Full-Text [PDF 1014 kb]
(238 Downloads)
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Type of Study: Orginal research papers |
Subject:
Genetics
ePublished: 2026/09/21
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