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Research Article: Effects of ovulin or pituitary extract treatments on spermatological, hematological, and biochemical responses of male Cyprinus carpio var. Sazan
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چکیده: (20 مشاهده) |
| The present study aimed to compare the effects of pituitary extract or Ovulin (containing s-GnRH and anti-dopamine) injection on spermatological parameters, hematological, and serum biochemical characteristics of male Cyprinus carpio var. Sazan. The male broodstocks were caught from the Caspian Sea and transferred to the Sijeval Center of Fish Propagation and Reconstruction of Genetic Reserves of Bony Fish in Bandar-e-Turkmen, Iran. After acclimation, the fish were divided into two experimental groups: one injected with pituitary extract (PT; 2 mg/kg) and the other with Ovulin (OV; 0.25 mL/kg). The blood samples were taken from the fish before and 12 hours after the injections (analyzed by two-way ANOVA); the sperm samples were also collected 12 hours after the injections (analyzed by t-test). The results showed that OV treatment significantly increased sperm pH, motility duration, and spermatocrit percentage, compared to PT treatment. The serum cortisol level was significantly increased in OV treatment after the injection. The serum glucose concentration and erythrocyte count were significantly increased after the injections, whereas the serum cholesterol, total protein, and sodium concentrations significantly decreased. The blood hemoglobin, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin were significantly decreased after the injection. There were no significant changes in blood leukocyte counts, serum potassium, calcium, albumin, triglyceride, and blood mean corpuscular hemoglobin concentration among the treatments (p>0.05). In conclusion, OV treatment serves as a beneficial alternative to PT extract for stimulating final spermatozoa maturation in male Sazan, without causing any health issues. |
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متن کامل [PDF 496 kb]
(7 دریافت)
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نوع مطالعه: پژوهشي |
موضوع مقاله:
Biology & physiology
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