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Research Article: Effect of lemon pomace inclusion on growth, immune response, anti-oxidative capacity, intestinal health, and disease resistance against Edwardsiella tarda infection in Nile tilapia
(Oreochromis niloticus)
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I.E.Ali. Gehan1   , A.A.Naena. Nehal2 , M.El-Shenawy. Abeer *3 , M.A.Khalifa. Amal4 |
1- 1Biochemistry Unit, Animal Health Research Institute, Kafrelsheikh Branch – Agriculture Research Center (ARC)
2- 2Bacteriology Unit, Animal Health Research Institute, Kafrelsheikh Branch – Agriculture Research Center (ARC)
3- Biochemistry, Nutritional Deficiency Diseases and Toxicology Unit, Animal Health Research Institute, Kafrelsheikh Branch – Agriculture Research Center (ARC) , abeer_elshenawy70@yahoo.com
4- 4Microbiology Unit, Animal Health Research Institute, Kafrelsheikh Branch – Agriculture Research Center (ARC) |
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Abstract: (466 Views) |
| This trial aimed to investigate the impact of dietary lemon pomace inclusion on growth, immune response, anti-oxidative capacity, intestinal health, and disease resistance against Edwardsiella tarda in the Nile tilapia. The fish weighted (20±5 g) were randomly allocated into three groups and fed diets containing varying amounts of dried lemon pomace powder (0[control], 1, and 2%) for 10 weeks. After this period, the fish were challenged with E. tarda. The bacterium was isolated from naturally infected fish from fish farms in the Kafr- Elsheikh governorate, Egypt with a rate of 14%, and its virulence genes (cds1, qse C, and pvsA) were detected using PCR. It was observed that 1 or 2% of lemon pomace dietary addition improved productive performance compared to control. Also, 1 or 2% of lemon pomace dietary addition reduced serum glucose, cortisol, triglycerides and cholesterol concentrations, while increasing the serum catalase and superoxide dismutase activities and improving phagocytic, lysozyme, and bactericidal activities in a dose-dependent manner compared to the control group. Moreover, 1 or 2% of lemon pomace dietary addition increased the length of intestinal villi and goblet cell number in a dose-dependent manner of different intestinal portions compared to the control group. The highest survival (80%) with the lowest mortality (20%), morbidity (56.6%), and re-isolation (30%) rates after E. tarda infection was observed in the group fed a diet containing 2.0% lemon pomace followed by 1.0% lemon pomace group, while the worst rates were recorded in the control fish. |
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| Keywords: Nile tilapia, Lemon pomace powder, Growth performance, Immune response, Edwardsiella tarda |
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Full-Text [PDF 900 kb]
(472 Downloads)
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Type of Study: Orginal research papers |
Subject:
Biochemistry
ePublished: 2024/12/2
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