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Anticancer effect of Dunaliella salina under stress and normal conditions against skin carcinoma cell line A431 in vitro
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Mo. Emtyazjoo   , Z. Moghadasi * , M. Rabbani , Ma. Emtyazjoo , S. Samadi , N. Mossaffa |
| , Ziba_mg2000@yahoo.com |
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Abstract: (14716 Views) |
| Dunaliella salina, a green microalga, has been consumed as food and
medicine for a long time.The anti-oxidant and anticancer effects are related to
more production of β- carotene under stress conditions compared with normal
circumstances.The scope of this study was survey of anticancer effect of the
ethanol extract of Dunaliella salina algae under stress(EDSS) and normal (EDSN) conditions on death rate of
skin carcinoma cell line A431 by
MTT [3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide] method in
vitro. Our results showed that the β-carotene amount in stress situation was 1.46
times more than in normal condition.The cytotoxic effect of EDSS exposed to 6.25,
12.5, 25, 50, 100 µg/ml in 6, 24, 48 h which was performed by MTT assay showed
that there was a significant difference in the various time except in 6 h in
both conditions.The results of compared death rate of A431cells exposed to
various concentrations of EDSS and EDSN presented significant difference in 6, 24,
and 48 h after exposure. The LC50 of
EDSS in 24 and 48 h were 15.4 and 0.4 µg/ml , respectively and 478.6 and 46.4
µg/ml for EDSN. |
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| Keywords: Dunaliella salina, Anticancer, A431 cell line, MTT assay, β-carotene |
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Full-Text [PDF 1024 kb]
(7545 Downloads)
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Type of Study: Orginal research papers |
Subject:
Biology & physiology
Received: 2012/06/10 | Accepted: 2013/09/28 | Published: 2013/09/28
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