[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 21, Issue 6 (2022) ::
IJFS 2022, 21 Back to browse issues page
Research Article: Efficiency of monovalent Vibrio alginolyticus formaldehyde-killed vaccine on the immune responses and protection of Asian seabass (Lates calcarifer) juveniles against Vibriosis
M. Ahangarzadeh, H. Houshmand *, S. Kakoolaki, A. Sepahdari, M. Ghorbanpoor, A. Ajdari, S. Nazemroaya, M. Zabayeh Najafabadi, M. Torfi Mozanzadeh, A.S. Sadr
South of Iran Aquaculture Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran. , houshmand.hossein@gmail.com
Abstract:   (243 Views)
Vibrio alginolyticus causes severe health problems in marine fish production each year. Among various therapeutic strategies, vaccination is the most economic, efficient and environmentally-friendly approach against microbial infections. Evaluation of formalin-killed Vibrio alginolyticus vaccine aquired from native isolates was carried out using a total of 200 Asian seabass juveniles (55±6.43 g), which were divided among three groups, including (I) control (non-vaccinated), (II) vaccinated with killed V. alginolyticus and (III) killed vaccine with oral booster. Fish were vaccinated intraperitoneally. Blood samples were taken from fish in each group at three, five, and eight weeks after immunization to assess the antibody levels against V. alginolyticus infection. The efficacy of the killed vaccine was appraised five weeks after the start of the initial vaccination by challenging with twofold LD50 (3.66×108) equivalent of the live suspension of V. alginolyticus through intraperitoneal injection. The results of ELISA showed that there were not any significant differences in antibody response among different groups before vaccination (p>0.05). Also, the mean antibody titer of the group immunized killed-V. alginolyticus with oral booster was significantly higher than the other groups five weeks after the vaccination. The vaccined fish demonstrated higher survival rates than the control with relative percent survival (RPS) of 84.62 and 76.92%, respectively. It is concluded that vaccination could be an effective method to protect farmed Asian sea bass against vibriosis caused by pathogenic V. alginolyticus.
Keywords: Vibriosis, Vibrio alginolyticus, Killed vaccine, Antibody titer, Survival rate
Full-Text [PDF 356 kb]   (71 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2022/02/8 | Accepted: 2022/05/29 | Published: 2023/02/22
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ahangarzadeh M, Houshmand H, Kakoolaki S, Sepahdari A, Ghorbanpoor M, Ajdari A, et al . Research Article: Efficiency of monovalent Vibrio alginolyticus formaldehyde-killed vaccine on the immune responses and protection of Asian seabass (Lates calcarifer) juveniles against Vibriosis. IJFS 2022; 21 (6) :1367-1382
URL: http://jifro.ir/article-1-4976-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 6 (2022) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4549