[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit ::
:: Volume 21, Issue 6 (2022) ::
IJFS 2022, 21 Back to browse issues page
Research Article: Alterations in Hematological indices, histopathology and p450 gene expression in stellate sturgeon (Acipenser stellatus Pallas, 1811) fingerlings exposed to different salinities levels and ammonia
A. Mansourghanaei, H. Khara *, H. Vahabzadeh Roudsari, Z. Pajand, M. Ahmadnezhad
Department of Fishery Science, Faculty of Natural Resources, Lahijan Branch, Islamic Azad University, Lahijan, Iran. , h.khara1974@yahoo.com
Abstract:   (296 Views)
This study aimed to combined effects of salinity and ammonia on physiological responses, liver enzymes, P450 gene expression, and gill and liver Histopathological of juvenile stellate sturgeon (Acipenser stellatus). To this end, a two-stage experiment was carried out. At the first stage, the lethal concentration (LC50 96h) of ammonia in salinities 0 ppt: 7.42 mgL-1, 4ppt:8.24 mgL-1, 8ppt: 9.60 mgL-1, and 12 ppt: 10.22mgL -1 to juveniles of stellate sturgons was determined. At the second stage, 360 juveniles of stellate sturgeons (with a mean weight and length of 15.23±2.17 g and 17±1.96 cm) were exposed to half (50%) median lethal concentration (LC50-96h) (3.71, 4.12, 4.80, and 5.11 mgL-1) under the same salinities for 4 days (8 treatments with 3 replicates a piece). Maximum blood indices (CBC) were observed in combined treatments with salinity and ammonia (P≤0.05). Blood stress indicators (Cortisol, Glucose, Lactate) and blood serum immunity indices (IgM, Lysozyme, Total Immunoglobulin) decreased and increased significantly with increasing salinity and ammonia in combination treatments, respectively (P≤0.05). The activity of GPX and SOD antioxidant enzymes increased in combined salinity and ammonia treatments but CAT decreased (P≤0.05). liver enzyme levels of ALT and AST in the blood serum of juvenile of stellate sturgeons increased in combined salinity and ammonia treatments(P≤0.05). But a significant decrease in the levels of ALP and LDH enzymes was observed (P≤0.05). The highest gill damage was observed in salinity of 12 p p t and also combined treatment of salinity of 12 p p t with ammonia. The most widely noticed symptoms included, Necrosis of Secondary lamellae, Disruption of cartilaginous core. Destruction of the pilar apparatus and congestion in the lamellae, Necrosis of primary lamellae and Fusion of some secondary lamellae. Liver tissue of juvenile stellate sturgeons (A. stellatus) was severely affected by high salinity alone and with ammonia. Complications such as Melanomacrophage center, Necrosis, Sinusoid and Karyolysis were more common. The hepatic P450 gene expression increased from Treatment 5 through Treatment 8 (p<0.05), as the highest gene expression was related to Treatment 8. This suggests that exposure to ammonia along with salinity stress activates the body's internal defense system. Therefore, brackish or saline water at concentrations (ammonia concentrations obtained at different salinities) proposed in this study can be used in aquaculture systems for the juveniles of stellate sturgeon. Moreover, Obtained concentrations of ammonia at different salinities in this study can help the organizations in charge of stock enhancement find the best places for introducing starry sturgeons to a lake or
Keywords: Acipenser stellatus, Ammonia, Salinity, Hematological indices, Histopathological, Gene expression p450
Full-Text [PDF 881 kb]   (132 Downloads)    
Type of Study: Orginal research papers | Subject: aquaculture
Received: 2021/12/31 | Accepted: 2022/08/15 | Published: 2023/02/22
Send email to the article author

Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mansourghanaei A, Khara H, Vahabzadeh Roudsari H, Pajand Z, Ahmadnezhad M. Research Article: Alterations in Hematological indices, histopathology and p450 gene expression in stellate sturgeon (Acipenser stellatus Pallas, 1811) fingerlings exposed to different salinities levels and ammonia. IJFS 2022; 21 (6) :1343-1366
URL: http://jifro.ir/article-1-4934-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 6 (2022) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4549