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:: دوره 21، شماره 6 - ( 1401 ) ::
جلد 21 شماره 6 صفحات 1416-1397 برگشت به فهرست نسخه ها
Research Article: Effect of enzyme type, enzyme to substrate ratio, temperature and hydrolysis time on the yield and antioxidant effect of Saurida tumbil protein hydrolysate
چکیده:   (1517 مشاهده)
It has become a hot topic to make optimal use of fisheries products and make them value added as well as find natural food substances which can effectively inhibit oxidative stress and improve human health. Given this, Saurida tumbil muscle was hydrolyzed by Alcalase and Papain at two concentrations of 2 and 4%, and at two hydrolysis times of 90 and 180 min and their antioxidant properties were compared. Antioxidant activity of the protein hydrolysates were investigated by ferrous chelating and ferric reducing, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl free radical scavenging activity tests. Samples hydrolyzed by Papain showed higher protein recovery than those hydrolyzed by Alcalase (59.9% versus 20.06%). The protein hydrolysate prepared by Papain enzyme showed significantly higher activity in removal of DPPH (IC50=2.44 mg/mL) and hydroxyl (IC50=3.93 mg/mL) free radicals than those prepared by Alcalase enzyme (DPPH IC50=2.80 mg/mL, hydroxyl IC50=8.59 mg/mL). ABTS radical scavenging activity did not show any significant difference between hydrolyzed samples with Papain or Alcalase enzymes. Hydrolyzed samples with Alcalase enzyme showed higher ferrous chelating activity (IC50=5.33 mg/mL) and ferric reducing activity (optical absorption=0.13±00 at 700 nm) than those hydrolysate prepared by Papain enzyme. Generally, the type of enzyme, its concentration and the time of hydrolysis affected the yield and antioxidant properties of Saurida tumbil muscle protein hydrolysates and hydrolysis with papain at concentration of 4% for 180 min provided the best properties.
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نوع مطالعه: پژوهشي | موضوع مقاله: Fish processing
انتشار الکترونیک: 1401/12/3
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Bahram S, Khezri M, Javadian S. Research Article: Effect of enzyme type, enzyme to substrate ratio, temperature and hydrolysis time on the yield and antioxidant effect of Saurida tumbil protein hydrolysate. IJFS 2022; 21 (6) :1397-1416
URL: http://jifro.ir/article-1-4311-fa.html

Research Article: Effect of enzyme type, enzyme to substrate ratio, temperature and hydrolysis time on the yield and antioxidant effect of Saurida tumbil protein hydrolysate. مجله علوم شیلاتی ایران. 1401; 21 (6) :1397-1416

URL: http://jifro.ir/article-1-4311-fa.html



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دوره 21، شماره 6 - ( 1401 ) برگشت به فهرست نسخه ها
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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