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 پایگاه استنادی علوم جهان اسلام (ISC)

پایگاه اطلاعات جهاد دانشگاهی (SID) 

بانک نشریات کشور (مگیران)




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Journal DOI


:: Volume 22, Issue 3 (2023) ::
IJFS 2023, 22 Back to browse issues page
Research Article: Effect of solvent type on phytochemical properties of burdock (Arctium lappa) extract and their effect on some pathogenic bacteria strains in rainbow trout, Oncorhynchus mykiss
M. Moayyed , A.R. Eftekharian , M.S. Fereiduni , P. Akbary *
Fisheries Group, Marine Sciences Faculty, Chabahar Maritime University, Chabahar, Iran , paria.akbary@gmail.com
Abstract:   (1053 Views)

The extraction method and particularly the type of solvents used in the procedure are one of the most crucial steps for isolating antimicrobial compounds and extracts that potentially affect various microorganisms. This study aimed to evaluate the effects of water, ethanol (100%), methanol (100%), ethanol-water (50:50), and methanol-water (50:50) burdock (Arctiumlappa) root extracts (BE) on total phenol content and polyphenols as well as the antioxidant activity. In the next step, the solvent with the highest efficiency to have phenolic compounds were selected and employed to investigate the antibacterial effects of BE on the pathogenic bacterial strains (i.e. Yersinia ruckeri, Lactococcus garvieae, Pseudomonas putida and Aeromonas hydrophila) in rainbow trout (Oncorhynchus mykiss) by using disc diffusion test and tube dilution techniques. Maximum mean total phenolic content and antioxidant capacity were detected in the water-methanol mixture. Additionally, High-performance liquid chromatography(HPLC) analysis revealed that both hesperidin and trans-ferulic acid compounds existed in all solvents. The antimicrobial activity of water-methanol BE was varied based on the type of microorganism. In this context, Y. ruckeri as a gram negative bacterium was found to be the most susceptible one compared to other pathogens. According to our findings, water-methanol solvent was more efficient to isolate phenolic compounds and exert antioxidant capacity, as the level of phenolic compounds in the extract was increased. Moreover, Y. ruckeri was recognized as the most sensitive pathogen in all tests.

Keywords: Polar and nonpolar solvents, Arctium lappa root extract, Antibacterial, Rainbow trout, Phenol
Full-Text [PDF 488 kb]   (609 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2019/08/9 | Accepted: 2020/05/26 | Published: 2023/08/15
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Moayyed M, Eftekharian A, Fereiduni M, Akbary P. Research Article: Effect of solvent type on phytochemical properties of burdock (Arctium lappa) extract and their effect on some pathogenic bacteria strains in rainbow trout, Oncorhynchus mykiss. IJFS 2023; 22 (3) :493-510
URL: http://jifro.ir/article-1-4018-en.html

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Volume 22, Issue 3 (2023) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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