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Fertilizing ability of cryopreserved spermatozoa in the Persian sturgeon (Acipenser persicus) and stellate sturgeon (A. stellatus)
.A Alipour *, Sh. Baradaran Noveiri, M.R. Nowruzfashkhami, M. Pourkazemi
, alireza_alipour@yahoo.com
Abstract:   (3123 Views)
Motility of spermatozoa was studied on 12 and 7 specimens of Acipenser persicus and A. stellatus, respectively. The density measured to be 2.22±0.65x109ml-1 in A. persicus and 2.21±0.55x109ml-1 in A. stellatus. Semen samples were diluted with two extenders containing tris 118mM, sucrose 23.4mM, pH=8, egg yolk (20%), dimethyl sulfoxide (15%) and penicillin potassium (500IU/ml) and biociphus extender containing glycerol as a cryoprotectant at a ratio of 1:1 and then transferred to 0.5ml straws and frozen in a computer controlled low temperature apparatus and stored in liquid nitrogen for one week. To study fertilizing ability, the spermatozoa were then used to inseminate eggs after thawing. Mean sperm motility in fresh spermatozoa was 86.6% in A. persicus and 73.75% in A. stellatus which decreased to 32.2% (p<0.001) and 37.5% (p<0.001) in frozen spermatozoa, respectively. Also mean fertilization rate decreased from 90.4% to 30.7% in A. persicus and from 72% to 36.8% in A. stellatus.
Keywords: A. persicus, A. stellatus, Spermatozoa, Cryopreservation, DMSO, Glycerol
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Type of Study: Orginal research papers | Subject: Biology & physiology
Received: 2017/12/4 | Accepted: 2017/12/4 | Published: 2017/12/4
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Alipour ., Baradaran Noveiri S, Nowruzfashkhami M, Pourkazemi M. Fertilizing ability of cryopreserved spermatozoa in the Persian sturgeon (Acipenser persicus) and stellate sturgeon (A. stellatus). IJFS. 2009; 8 (1) :1-12
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Volume 8, Issue 1 (2009) Back to browse issues page
Iranian Journal of Fisheries Sciences Iranian Journal of Fisheries Sciences
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