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Phylogenetic analysis of viral hemorrhagic septicemia virus from recent outbreaks in some of the farmed rainbow trout, Oncorhynchus mykiss (Walbaum, 1792)
F. Fattahi *, M. Akhlaghi, A. Mohammadi, S. Soltanian, N. Shahbazian
quatic Animal Health & Diseases Department, School of Veterinary Medicine, Shiraz University, Shiraz, Iran , fatemehfattahi@yahoo.com
Abstract:   (433 Views)
During a fish disease outbreaks in 2015-2016 suspected to viral hemorrhagic septicemia virus (VHSV), moribund rainbow trout fries were obtained from fish farms. Samples of kidney, heart and spleen tissues were taken  for examination. The full coding region of the G gene was amplified by RT-PCR reaction. Genotyping was carried out by phylogenetic analysis with reference sequences of four VHS genogroup. A phylogenetic tree was constructed with the maximum likelihood method and the Kumara 2- parameter substitution model by using MEGA software version 6.06. A maximum likelihood phylogenetic analysis reveals that all the Iranian VHS   strains belonged to clade Ia-2 of genotype Ia. The intra- sequence comparison of Iranian VHS strains ranged from 99.1% to 100% (average was 99.55%). In this study, two strains (Chaharmahal and Bakhtiari (1) and Chaharmahal and Bakhtiari (3) showed 100% identity but 0.1% differences with Isfahan strain. The Iranian strains belonging to European genogroup are related (99% identity) to VHSV from recent Italian strains (VHSV/O.mykiss/I/TN/133/Apr10).

Keywords: VHSV, Phylogenetic, Rainbow trout, Iran
Full-Text [PDF 1237 kb]   (151 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2017/10/21 | Accepted: 2018/06/9 | Published: 2020/05/1
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Fattahi F, Akhlaghi M, Mohammadi A, Soltanian S, Shahbazian N. Phylogenetic analysis of viral hemorrhagic septicemia virus from recent outbreaks in some of the farmed rainbow trout, Oncorhynchus mykiss (Walbaum, 1792). IJFS. 2020; 19 (3) :1384-1395
URL: http://jifro.ir/article-1-2994-en.html


Volume 19, Issue 3 (2020) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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