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IJFS 2019, 18 Back to browse issues page
Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C
S Keivanloo, M Sudagar , M Mazandarani
Gorgan University of Agricultural Sciences and Natural Resources , sudagar_m@yahoo.com
Abstract:   (196 Views)

In the present study, cryopreservation of common carp (Cyprinus carpio) embryos was studied using a stepwise cooling protocol. Embryos at heartbeat stage were selected, placed in 15- ml plastic tubes and stored at -2 °C for 1, 7, 14, and 21 days in 2 different cryopreservation solutions: S1 (methanol + dimethyl sulfoxide + sucrose) and S2 (methanol + propylene glycol + sucrose). Embryo viability was assessed by survival rate, counting live larvae and number of failed eggs under a stereomicroscope.  The results showed that storage of common carp embryos was possible for up to 7 days at -2 °C, while the survival did not occur when storage period were 14 and 21 days. The combination of methanol with propylene glycol (S2) gave higher survival rate after 1 and 7 days stored at -2 °C. Further studies are needed in order to extend the storage time and to improve the survival rate for this species.

Keywords: Cryopreservation, Common carp, Cryoprotectant
Full-Text [PDF 241 kb]   (45 Downloads)    
Type of Study: Orginal research papers | Subject: aquaculture
Received: 2017/01/3 | Accepted: 2017/04/22
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Keivanloo S, Sudagar M, Mazandarani M. Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C. IJFS. 2019; 18 (4) :1036-1045
URL: http://jifro.ir/article-1-2526-en.html


Volume 18, Issue 4 (9-2019) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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