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:: Volume 19, Issue 3 (2020) ::
IJFS 2020, 19 Back to browse issues page
Definite diagnosis of viral haemorrhagic septicaemia infected farmed rainbow trout and histopathologic study of acutely diseased fish in Iran
R. Rahnama *, R. Peyghan, M.R. Seyfi Abad Shapouri, A. Rezaie, N. Shahbazian
Shahid Chamran University of Ahvaz, Ahvaz, Iran , royarahnama60@yahoo.com
Abstract:   (80 Views)
Viral haemorrhagic septicaemia (VHS) is one of the most important viral diseases in rainbow trout that has caused great losses to the Iranian rainbow trout aquaculture industry in few recent years. In the early autumn of 2015, in order to identify the possible causes of loss, farmed fish were sampled from the epicenter where massive mortalities and economic losses had occurred. Three samples were collected from relevant tissues (i.e. liver, kidney, spleen, heart, and brain) of the rainbow trout with clinical signs of VHS disease. The samples were stored in 70% ethanol, Eagle’s minimum essential medium (EMEM) and 10% formalin to perform RT-PCR, cell-culture and histopathology examinations, respectively. A virus was isolated using fish cell lines inoculated with a homogenate of the internal organs. Sequencing analysis of truncated N gene confirmed the existence of VHS virus. RT-PCR and histopathology tests indicated the suitability and simplicity of the test as a paraclinical diagnostic method for VHS virus in the region. Haemorrhage, necrosis and other changes in histological sections of the diseased fish demonstrated pathological changes identical with the typical VHS pathology in target organ of the virus (i.e. heart, spleen, liver and kidney). To our knowledge, this is one the first studies that fully focused on the identification of VHSV from farmed trout in Iran by using histopathology examinations.
Keywords: Histopathology, Rainbow trout, RT-PCR, Viral haemorrhagic septicaemia
Full-Text [PDF 1078 kb]   (14 Downloads)    
Type of Study: Orginal research papers | Subject: fish disease
Received: 2016/12/24 | Accepted: 2017/02/6
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Rahnama R, Peyghan R, Seyfi Abad Shapouri M, Rezaie A, Shahbazian N. Definite diagnosis of viral haemorrhagic septicaemia infected farmed rainbow trout and histopathologic study of acutely diseased fish in Iran. IJFS. 2020; 19 (3) :1556-1572
URL: http://jifro.ir/article-1-2513-en.html


Volume 19, Issue 3 (2020) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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