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IJFS 2016, 15 Back to browse issues page
Chitosan extracted from the Persian Gulf chiton shells: Induction of apoptosis in liver cancer cell line
H Rasti, K Parivar, J Baharara *, M Iranshahi, F Namvar
Professor, Research Center for Animal Development, Applied Biology & Biology Department, Mashhad Branch, Islamic Azad University, Mashhad, Iran , baharara78@gmail.com
Abstract:   (3603 Views)

Here for the first time, we investigated the cytotoxic effects of the chitosan extracted from the Persian Gulf Chiton shell (Acanthopleura vaillantii) on liver cancer cell line (HepG2). Chitosan extraction was implemented following this method: chitin was produced by demineralization and deproteinization procedure, and the extracted chitin was converted into soluble chitosan using deacetylation method. The cytotoxic effects of extracted chitosan were evaluated using four different tests, including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Annexin V-FITC, propidium iodide (PI) staining, 4chr('39'),6-diamidino-2-phenylindole (DAPI) staining, and Caspase activity analysis. The IC50 inhibitory concentrations of chitosan were obtained at 250 µg/mL after 24 h. Chitosan clearly inhibited the growth of hepatocarcinoma cells in vitro in a dose-dependent manner. For detecting the induced cell apoptosis, HepG2 cells were treated with 125, 250 and 500 µg/ml of chitosan for 24 h. According to the result of Annex in V/PI kit, in 125, 250, and 500 µg/ml of chitosan, 28.2, 49.1, and 83.3% of HepG2 cells undergone late apoptosis, respectively. The morphology of treated cells by DAPI staining showed non uniform plasma membrane and DNA fragmentation compared to untreated cells with perfect nucleus. The analysis of cell cycle using flow cytometry demonstrated that the rate of sub-G1 peak was increased to 52.7%. Both caspase-3 and -9 activities increased by the extracted chitosan, but it was only significant for caspase-3. The results of the present study suggested that the extracted chitosan has efficient cytotoxicity on HepG2 cells. Therefore, the extracted chitosan from the shell of the Chiton may be considered as a futuristic natural product regarding the treatment of liver cancer..

Keywords: Chiton, Chitosan, Persian Gulf, Natural product, Apoptosis
Full-Text [PDF 344 kb]   (1757 Downloads)    
Type of Study: Orginal research papers | Subject: Biology & physiology
Received: 2015/11/9 | Accepted: 2016/01/6 | Published: 2016/10/23
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Rasti H, Parivar K, Baharara J, Iranshahi M, Namvar F. Chitosan extracted from the Persian Gulf chiton shells: Induction of apoptosis in liver cancer cell line. IJFS. 2016; 15 (4) :1362-1378
URL: http://jifro.ir/article-1-2019-en.html

Volume 15, Issue 4 (2016) Back to browse issues page
مجله علوم شیلاتی ایران Iranian Journal of Fisheries Sciences
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