For this study one species of Shyrnidae family caught along Persian Gulf in Bandarabbas city. Sphyrna lewini were transferred to the laboratory on spring 2007. The antimutagenic activity of the Methanolic and Chloroforamic extracts of muscle, liver and cartilage of Sphyrna lewini with the Ames test was investigated. The use of antimutagens and anticarcinogens in everyday life is the most effective procedure to prevent human cancer and genetic diseases. Since angiogenenesis is a key factor in tumor growth, inhibiting this process is one way to treat cancer. In this study the antimutagenic effect of the Chloroformic and Methanolic extracts of muscle, liver and cartilage on the damage induced by two mutagens was studied. The results driven from this study were inhibitory effect of two extracts. The highest antimutagenic effect was determined in the Potassium Permanganate and Sodium Azide as a mutagen was Methanolic extracts of cartilage . There is a general correlation between mutagenesis and the initiation stage of carcinogenesis. Mutagens appear to initiate the process by inducing the primary DNA lesion. These are called initiators and the damage they cause is generally irreversible.