1 1562-2916 Iranian Fisheries Research Organization 3715 Fish assemblages and habitat ecology of River Pinder in central Himalaya, India Agarwal N.K. b Rawat U.S. c Singh G. d b Garhwal University India, Fish Reproduction and Conservation Biology Research Laboratory, Department of Zoology, School of Life Science, India c Sri Dev Sumen Uttarakhand University, Badshahithaul, India d Garhwal University India, Fish Reproduction and Conservation Biology Research Laboratory, Department of Zoology, School of Life Science, India 1 1 2019 18 1 1 14 25 12 2018 25 12 2018 Snow-fed River Pinder -a tributary of River Alaknanda in central Himalaya was explored for fish assemblages and habitat specificity. Altogether 27 fish species were reported from three orders, four families and nine genera. Cypriniformes order was dominating followed by Siluriformes and Salmoniformes. Shannon-Weiner diversity index (3.09 to 4.10) and Simpson index of diversity (0.81 to 0.92) of four sites specified strong relationship with species richness. The distribution of fish species showed interesting patterns, 33% species were common to all four sampling sites while 14.80% were restricted to single site and the remaining species were randomly distributed among two or three sampling sites. Habitat variability in the river significantly influenced the species assemblage structure. About 7.40% species were found common to all habitats while 3.70% species were restricted to only single habitat type. The remaining 88.90% of species were dwelling between two to three habitat types. Deep pools recorded maximum species richness followed by shallow pools, while least species richness was recorded in cascade habitats. The conservation status of fish fauna of the river was ascertained by CAMP (Conservation Assessment and Management Plan). Out of 27 species, the status of 8 species was not assessed due to data being deficient, 7 species were categorised as lower risk near threatened, 6 as vulnerable, 5 as endangered while 1 species was exotic. 
3716 Growth yield, carcass traits, biochemical and non- specific immune parameters in grey mullet, Mugil cephalus Linnaeus, 1758 under cyclic starvation and L- carnitine supplementation Akbary P. e e Fisheries Group, Department of Marine Sciences, Chabahar Maritime University. 1 1 2019 18 1 15 29 25 12 2018 25 12 2018 The main objective for the present research was to assess the effect of cyclic starvation and L- carnitine supplementation on growth (body final weight, hepatosomatic index (HSI), daily growth rate (DGI), feed conversion ratio (FCR) and voluntary feed intake (VFI)), body composition (crude protein, crude lipid, ash and moisture), biochemical (plasma total protein, glucose, cholesterol, triglyceride and liver glycogen) and immunological (lysozyme activity) parameters in grey mullet. To satisfy the foregoing end, a total of 240 fish with an average weight of 1.33±0.26 g were randomly divided into four groups (three replicates, 20 fish per tank). Two groups were fed on a daily basis and the other two ones were kept starved for 7 days once in 2 weeks. Two groups were fed a carnitine free basal diet and the remaining were fed a diet with 800 mg carnitine kg-1. Cyclic starvation led to significantly decreased body final weight, DGI, FCR, total protein, triglyceride and cholesterol. On the contrary, no significant differences were found across all groups for FCR, HSI, glucose, liver glycogen and lysozyme concentrations. At the same time, the combined effects of cyclic starvation and carnitine supplementation were manifested in DGI and VFI parameters. In light of the above results, it can be noted that in both feeding regimes, growth, feed utilization and carcass quality in grey mullet increased upon adding 800 mg carnitine kg-1 diet.  3717 Viral nervous necrosis (VNN) an emerging disease caused by Nodaviridae in aquatic hosts: Diagnosis, control and prevention: A review Zorriehzahra M.J. f Adel M. g Dadar M. h Ullah S. i Ghasemi M. j f Aquatic Animal Health and Diseases Department, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, I.R. Iran. g Aquatic Animal Health and Diseases Department, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, I.R. Iran. h Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, I.R. Iran. i Fisheries and Aquaculture Lab, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan. j Iranian Fisheries Science Research Institute (IFSRI), Inland Water Aquaculture Research Center, Bandar Anzali, I.R. Iran. 1 1 2019 18 1 30 47 25 12 2018 25 12 2018 Betanodavirus is one of the two genera making up the family Nodaviridae and is the etiological agent of viral nervous necrosis (VNN, also known as viral encephalopathy and retinopathy or VER). The virus infects a large range of host species in more than 50 species of marine and freshwater fish worldwide from different geographical areas and the known host range continues to expand as new species of fish are used for aquaculture. The disease is characterized by vacuolating necrosis of neural cells of the brain, retina and spinal cord and causes up to 100% mortality in larval and juvenile fish, and can cause significant losses in older fish. The lack of knowledge about control and prevention of the disease makes the problem serious and impedes development of management approaches. Therefore this review focuses on current knowledge and future perspectives of viral nervous necrosis in the aquaculture industry with special focus on the type of diagnosis, control and prevention of the disease. 3718 Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay Alizadeh Sabet H.R. k Kalbassi M.R. l Pourkazemi M. m Sadeghizadeh M. n k Coldwater Fishes Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Training and Extension Organization ,Tonekabon, Iran l Department of Aquaculture, Tarbiat Modares University, Faculty of Marine Sciences, Noor, Iran m Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Training and Extension Organization, P.O. Box14155-6116, Tehran, Iran. n Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran 1 1 2019 18 1 48 59 25 12 2018 25 12 2018 Caspian trout (Salmo trutta caspius Kessler, 1877) as endemic migratory fish, possessing great ecological economic and biodiversity value. Among the habitat drivers, temperature related effects on the genome were considered in this research. During the embryonic developmental stages, genomic alterations of single cells were assessed by Comet assay. After artificial propagation of wild broodstocks, the batches of eggs, were transferred to incubators with constant temperatures, REDD water recycling, 3 stages water filtration, UV-C application on effluent of each incubator and DO–pH–temperature control. Samples were collected from normal (8°C), cold (4°C) and warm (12°C) treatments on eyed egg, alevin and larval stages. Single Cell Gel Electrophoresis were applied on samples for evaluation of Comet Tail length (TL), DNA in tail (DT) and Tail Moment (TM) indices as DNA damage markers. Comparison between mean values of Comet TL index indicated 85.19 ±9.2, 9.88 ±2.3 and 256.54±14.6 for 4, 8 and 12°C treatments, respectively. DT were 18.07±6.3, 9.72±1.9 and 31.47±2.8 and then TM were measured as 15.27± 4.1, 1.21±0.2 and 95.25±8.6 for 4, 8, and 12°C treatments, respectively. Significant difference between thermal treatments (p<0.05) observed. Warm treatments demonstrated greater DNA damage in compare with others. TM explained better information in comparison with TL and DT in all analyzed samples. In conclusion, temperatures out of the range of the optimal conditions could significantly affect Caspian trout genome which could be the subject of management considerations pertaining to Caspian trout stocks rehabilitation and captive rearing strategies.[PN1]  3719 Stock identification of Arabian yellow fin sea bream (Acanthopagrus arabicus) using shape of otolith in the Northern Persian Gulf and Oman Sea Doustdar M. o Kaymaram F. p Seifali M. Jamili S. Bani A. o Department of Marine Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. p Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran. Plant Sciences Department, Faculty Biological Sciences, Alzahra University, Vanak, Tehran, Iran. Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran. Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran 1 1 2019 18 1 60 70 25 12 2018 25 12 2018 Otolith shape analysis is one way to identify stocks of different fish species in the marine environment. Length, width, area, perimeter, form factor, aspect ratio, roundness, circularity, ellipticity and rectangularity analyses of otoliths were undertaken to assess patterns of spatial and temporal stock structure of a wide-ranging fish, the Arabian yellow fin sea bream Acanthopagrus arabicus. Fish were sampled from 125 stations across the distribution range of the species in the Northern Persian Gulf and Oman Sea from June 2014 to May 2016. Analysis of morphometric parameters of otolith showed the minimum size in Khuzestan waters and the West Strait of Hormuz and the maximum size in the East Strait of Hormuz. In Bushehr waters, form factor showed the lowest and circular factor showed the highest frequency. These findings are in agreement with the irregularity in otolith margin of this area. The highest aspect ratio of otolith indicated higher growth in Khuzestan Waters. The thinner and longer otoliths were identified in the East Strait of Hormuz region. Further, rectangularity ratio factor in the Bushehr and West Strait of Hormuz waters was higher in comparison with other regions and this finding confirmed the quadrilateral otolith shape of this region. There are significant differences among otolith morphometric variables of the A. arabicus (p<0.05). The result of discriminant analysis on morphometric parameters indicated that 53/8 percent were in their geographic location accurately.  3720 Oral administration of garlic powder (Allium sativum) on growth performance and survival rate of Carassius auratus fingerlings Dadgar Sh. Seidgar M. Nekuiefard A. Valipour A.R. Sharifian M. Hafezieh M. Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Urmia, Iran National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Urmia, Iran Inland waters research center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Anzali, Iran Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran 1 1 2019 18 1 71 82 25 12 2018 25 12 2018 This study was carried out to evaluate the effect of different levels of garlic (Allium sativum) on growth survival and nutritional characteristics of goldfish (Carassius auratus). For this purpose, 180 goldfish with an average initial weight of 1.18± 0.08 g were introduced randomly into 12 aquaria (50×30×40 cm) in 4 groups, with 15 fish in each group. Treatments were fed on a basal diet for one week, followed by experimental diets for eight weeks. Garlic powder was added at levels of 0 (Control), 0.5, 1 and 1.5 mg kg-1 to commercial diet. At the end of the experiment, growth and survival rates and feed performance were evaluated. There was no significant difference in growth rate and feed efficiency between treatments, although they were better in the 0.5% level. The highest survival rate was achieved in the 0.5% treatment that showed a significant difference compared to the control diet (p<0.05). The best growth performance and feed efficiency were obtained in fish fed the 0.5% garlic powder diet.  3721 Investigation of physicochemical factors, chl-a and primary production in Mahshahr Creeks (Northwest Persian Gulf) Ghotbeddin N. Mosavi Dehmordi L. Department of Fisheries, College of Agriculture and Natural Resources, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran. Khatam Alanbia University of Technology, Faculty of Natural Resources, Department of Fisheries, Behbahan, Iran. 1 1 2019 18 1 83 94 25 12 2018 25 12 2018 The study on physicochemical factors and primary production of the aquatic ecosystems is important to know the characteristics of the environment and capacity of recharge. Mahshahr Creeks are the most important creeks in the Northwest Persian Gulf, Iran. In this study, variations in physicochemical factors, chl-a, and primary production in creeks of Mahshahr, Zangi, and Moussa were evaluated for 2 months of every season of 2014. The following variables were analyzed: nitrate, phosphate, nitrite, silicate, temperature, salinity, DO, pH, conductivity, turbidity, light, chl-a, primary production and phytoplankton abundance. The maximum amount of chl-a (1.6 mg m-3) was observed in Moussa Creek in April while the minimum amount (0.1 mg m-3) was seen in Moussa in July. The highest (1.19 gc m-2 day-1) and lowest (0.1gc m-2 day-1) amount of primary production were observed at the entrance of Moussa Creek in April and in Zangi Creek in November, respectively. The maximum and minimum abundance of phytoplankton was observed in April and October, respectively in all study areas. The results of correlation test between the studied factors revealed that primary production has a significant relationship with nitrite, chl-a, phytoplankton abundance and light. Primary production in these creeks is relatively high in April and is attributed to the uptake of nutrients including nitrate and phosphate from shallow waters entering these creeks, sufficient light and suitable temperature, but in other months the production is low.  3722 The effects of Rosmarinus officinalis essential oil on the quality changes and fatty acids of Ctenopharyngodon idella Roomiani L. Ghaeni M. Moarref M. Fallahi R. Lakzaie F. Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran Iranian Fisheries Science Research Institute, Tehran, Iran Iranian Fisheries Science Research Institute, Tehran, Iran 1 1 2019 18 1 95 109 25 12 2018 25 12 2018 The effects of the use of essential oil of Rosmarinus officinalis on the chemical quality and fatty acids of fish fillets of Ctenopharyngodon idella were investigated during frozen storage at -18 ºC for 6 months. Fish fillets were divided into three groups; the control (C) without rosemary essential oil, the second group with 0.2 % rosemary essential oil (A) and the third with 0.4 % (v/v) rosemary essential oil (B). According to compositional analysis of the rosemary essential oil by GC-MS, the α-pinene (31.91%) and 1, 8- cineol (14.66%) were the predominant components. Results showed that there were no significant differences of protein in group C and A, but differences were seen between A and B (p<0.05). A significant decrease in lipid was obtained throughout between groups during storage (p<0.05). There were no significant differences of pH between three groups at storage periods (p>0.05). Effect of rosemary essential oil showed that the least changed fatty acids were on polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA) and saturated fatty acids (SFA), respectively. Microbial results showed TVC content of fillets fish did not exceed the limit during storage period for A and B Groups (< 7 log CFU g-1). Rosemary essential oil at 0.2% and 0.4 % was effective in controlling the chemical compositions and fatty acids, but sensory attributes reveal a decreasing trend in the attributes like color, odour, taste, firmness and general acceptance for two groups (p<0.05), but group A indicated better scores than Group B at the end of the storage period. 3723 Effects of different cooking methods on minerals, vitamins and nutritional quality indices of grass carp (Ctenopharyngodon idella) Golgolipour S. Khodanazary A. Ghanemi K. Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. Department of Marine Chemistry, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran 1 1 2019 18 1 110 123 25 12 2018 25 12 2018 This study aimed to evaluate the nutritional value (proximate composition, fatty acid profiles, vitamins and minerals) contents and also nutritional quality indices (NQI)) of grass carp (Ctenopharyngodon idella) prepared according to common consumer techniques: raw, poached, steamed, microwaved, pan-fried and deep-fried (in olive oil). In comparison to raw fish fillets, when grass carp was cooked there was an increase in protein, lipid and ash contents. Cooking methods had no significant effect on total n-3 fatty acids except for frying fillets. Lowest and highest content of n-3 was shown in deep-fried and pan-fried samples, respectively. Total n-6 fatty acid of cooked samples increased in comparison to raw samples. Na, K, Mg, P and Zn contents of boiled fish fillets significantly decreased. None of cooking methods had a significant effect a vitamin D. However, vitamin A, B1 and B3 contents of cooked fish significantly decreased.  3724 Scale characteristics of the bloom event: A case study in the Iranian coastal waters of the Southern Caspian Sea Makhlough A. Nasrollahzadeh Saravi H. Eslami F. Keyhansani A.R. Caspian Sea Ecology Research Center (CSERC), Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran Caspian Sea Ecology Research Center (CSERC), Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran Caspian Sea Ecology Research Center (CSERC), Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran Caspian Sea Ecology Research Center (CSERC), Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran 1 1 2019 18 1 124 139 25 12 2018 25 12 2018 Nutrient enrichment in water and sediments due to excessive anthropogenic activities in recent years has caused excessive algal growth in the Caspian Sea. The current study was conducted to determine the abundance of phytoplankton community, the dominant species and chlorophyll-a [Chl-a] concentration during algal blooms in the Iranian coastal waters of Caspian Sea through four seasons from 2013 to 2014. The minimum and maximum phytoplankton abundance recorded were 73±31 and 505±55 million cells m-3 in summer and winter, respectively. The median concentration of Chl-a increased to 5.81 mg m-3 in autumn, as compared to the annual median value (2.43 mg m-3). The results indicated that the bloom started in autumn and it continued falling with a low concentration during winter (Chl-a: 2.59 mg m-3). The three species Stephanodiscus socialis, Binuclearia lauterbornii and Thalassionema nitzschioides were classified in medium bloom class (100-1000 million cells m-3) in spring, summer and autumn, respectively. While in winter Pseudonitzschia seriata (harmful species) and Dactyliosolen fragilissima were classified in medium bloom class with high relative frequency. The scaling of bloom abundance revealed that bloom initiation coincided with 10 million cells m-3 of the dominant phytoplankton species. The bloom at the regions with more than 100 million cells m-3 of total phytoplankton abundance and dominant species was overlapped with the bloom regions based on Chl-a concentration.  3725 Fish assemblage and structure as well as hydrological parameters at Karatoya Fish Sanctuary, Panchagarh, Bangladesh Md. Rashidul I. Md. Jewel M. Shirin S. Department of Fisheries Biology and Genetics, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh Department of Fisheries Biology and Genetics, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh 1 1 2019 18 1 140 162 25 12 2018 25 12 2018 Spatiotemporal variations in fish assemblage structure was studied from January to December 2015 in order to understand the impacts of the sanctuary on ichthyo-faunal diversity and to determine the indices and major hydrological factors in six sampling stations of Karatoya Fish Sanctuary sectioned in the River Karatoya. A total of 69 fish species were obtained from this sanctuary including 21 threatened species where Aspidoparia jaya, Pethia ticto, Puntius sophore, Canthophrys gongota and Barilius barna were the major contributory species (>4.17%) both in space and time. The uppermost species richness and abundance were viewed in January and lowest in May. Based on analysis of similarities (ANOSIM), fish assemblages were significantly different in all stations (p<0.001, R=0.15) and months (p<0.001, R=0.62). Through two-dimensional nonmetric multidimensional scaling (nMDS) and cluster analysis based on Bray-Curtis similarity index, assemblages were alienated into two groups at a value of 42% and 28% partition for station and month, respectively. Canonical Correspondence Analysis (CCA) recognized considerable relations between the number of fishes and hydrological parameters where dissolved oxygen (mg L-1) and water temperature (ºC) were the main leading factors in shaping the fish assemblage structure 3726 Effect of different levels of iron sulfate on some haematological parameters of ship sturgeon, Acipenser nudiventris Hosseini S.H. Kamali A. Yazadani M.A. Khara H. Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran, P.O.Box:14515/775 Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran, P.O.Box:14515/775 Department of Aquaculture, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran, P.O. Box: 41635-3464 Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Iran, P.O. Box: 1616 1 1 2019 18 1 163 172 25 12 2018 25 12 2018 The present study was conducted to examine different dietary levels of iron (FeSO4) on some haematological parameters of ship sturgeon, Acipenser nudiventris including red blood cells (RBC), white blood cells (WBCs), differential WBCs, hematocrit (Hct), Hemoglobin (Hb), the mean corpuscular hemoglobin (MCH) and the mean corpuscular hemoglobin concentration (MCHC), the mean corpuscular volume (MCV), immunoglobulin (IgM), ferritin, transferrin and plasma Fe2+. Four experimental treatments and one control group with three replicates were considered for the experiment. The experimental treatments were fish fed experimental diets containing different levels of FeSO4 i.e. 100, 200, 250, and 300 mg kg.diet-1. After 60 days, there were significant differences between experimental groups in terms of WBCs, lymphocytes, neutrophils, monocytes, eosinophils, MCH and MCHC, MCV, total plasma protein, IgM, ferritin, transferrin and plasma Fe2+ (p<0.05). In the treatment fed 100 mg.iron kg.diet-1, more levels of IgM and total plasma protein were observed compared to other experimental groups (p<0.05). The highest values of neutrophils, monocytes and MCHC were found in control fish whereas MCV, MCH and eosinophils values were higher in the treatment fed 200 mg.iron kg.diet-1 (p<0.05). Also, more WBCs were observed in 250 mg.iron kg.diet-1 treatment than in other groups (p<0.05). The values of lymphocytes, RBC, Hct, Hb, ferritin, transferrin and plasma Fe2+ were higher in fish fed 300 mg.iron kg.diet-1 compared to other experimental groups (p<0.05). In conclusion, our results showed that the supplementation of ship sturgeon diets with high levels of iron enhances the RBC, Hct, Hb, ferritin, transferrin and plasma iron.  3727 The synergistic influence of Holothuria arenicola extract and imidazole carboxamide on Cellosaurus cell line B16-F10 Baharara J. Nikdel N. Nezhad Shahrokhabadi Kh. Amini E. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran Cellular and Molecular Department, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran 1 1 2019 18 1 173 187 25 12 2018 25 12 2018 Skin cancer has been reported as a contemporary malignant cancer. Here, anti-cancer effects of sea cucumber extract (SCE) from Holothuria arenicola have been examined on melanoma cells and compared with imidazole carboxamide (Dacarbazine) as a chemotherapy medication against melanoma and Hodgkin's lymphoma. MTT assay and morphological analysis were performed to evaluate cytotoxic effects of H. arenicola extract. Also, several methods were exerted to detect cell dying by SCE and imidazole carboxamide. The MTT assay showed that B16F10 cells proliferation was blocked by SCE (IC50=31µg mlml-1) and imidazole carboxamide (IC50=1600 µg mlml-1) in a dose and time dependent manner. Apoptosis induction yield treatment occurred at IC50 concentration of SCE and imidazole carboxamide using DAPI staining, Acridine orange/Propodium iodide, PI flow cytometry and annexin/PI assay. The caspase colorimetric kit indicated that SCE and imidazole carboxamide could induce apoptosis through an intrinsic pathway. Collectively, our findings suggested that the methanolic SCE has more efficient cytotoxicity efficiency compared to imidazole carboxamide. Therefore, SCE may be considered as a futuristic marine natural product regarding prevention or treatment of melanoma malignancy. 3728 Short communication:Determination of some heavy metal levels in three freshwater fish in Keban Dam Lake (Turkey) for public consumption Caglar M. Canpolat O. Selamoglu Z. Department of Fundamental Science, Faculty of Fisheries, Firat University, Elazig, Turkey Department of Fundamental Science, Faculty of Fisheries, Firat University, Elazig, Turkey Department of Medical Biology, Faculty of Medicine, University of Nigde Ömer Halisdemir, Nigde, Turkey Department of Medical Biology, Faculty of Medicine, University of Nigde Ömer Halisdemir, Nigde, Turkey 1 1 2019 18 1 188 198 25 12 2018 25 12 2018 Due to industrialization, a number of factories and human population have increased rapidly. As a result, the amount of waste matter such as heavy metals released to the environment has been increased. Specifically aquatic systems are more sensitive to heavy metal pollution and the gradual increase in the levels of such metals in aquatic environments, mainly due to anthropogenic sources, have become a problem of primary concern (Ashraf et al., 2012). The natural aquatic ecosystems may extensively be contaminated with heavy metals released from domestic, industrial and other anthropogenic activities (Kamaruzzaman et al., 2011).   3729 Short communication:Zooplankton of Çat Dam Lake (Malatya-Turkey) with a new record for Turkish rotifers Lecane intrasinuata (Olofsson, 1917) Saler S. Bulut H. Karakaya G. Fırat University, Fisheries Faculty, 23119 Elazığ-Turkey Fırat University, Fisheries Faculty, 23119 Elazığ-Turkey Elazığ Fisheries Research Station, Elazığ-Turkey 1 1 2019 18 1 199 204 25 12 2018 25 12 2018 There are many studies on the zooplankton of Turkish lakes, many of which are about seasonal fluctuations, distribution and hydrological changes. In recent years many scientists focused their attention on the zooplankton of Turkish inland waters (Dumont and De Ridder, 1987; Segers et al., 1992).