1 1562-2916 Iranian Fisheries Research Organization 3125 aquaculture Effects of n3-HUFA enriched Daphnia magna on growth, survival, stress resistance, and fatty acid composition of larvae of Persian sturgeon (Acipenser persicus) Abedian Kennari A.M. Oveisipour M.R. Nazari R.M. 1 7 2007 7 1 1 14 09 12 2017 09 12 2017 Effects of Daphnia magna enriched with cod liver oil (CLO) as a source of highly unsaturated fatty acid (HUFA) on growth, survival, stress resistance, and fatty acid composition of the Persian sturgeon larvae were evaluated. Daphnia enriched for three different time periods (3, 6, and 9 hours) and non-enriched Daphnia were fed to the Persian sturgeon larvae (average weight 61.6±0.4mg) during 14 days. The highest n3-HUFA content was found in Daphnia enriched for 9 hours (0.69mg g-1 DW) and the highest n3-HUFA content of the larvae was also found in those larvae fed with Daphnia enriched for 9 hours (2.39mg/g-1DW). A significant growth difference between larvae fed with enriched and non-enriched Daphnia was observed (p<0.05), while survival rate did not significantly differ among the treatments (p>0.05). Furthermore, the highest pH stress resistance was found in those larvae fed with Daphnia enriched for 9 and 6 hours (p<0.05). A salinity stress test did not show significant differences among the treatments.
3109 fish disease Incidence of white spot disease (WSD) in Penaeus indicus farms in Bushehr Province, Iran Afsharnasab M. Dashtyannasab A. Yeganeh V. Soltani M. 1 7 2007 7 1 15 26 09 12 2017 09 12 2017 The incidence of white spot disease in farmed Penaeus indicus in Busheher region was evaluated by analyzing 200 shrimp individuals between June and September, 2005. The samples were examined for clinical sign including observation of white spots on the cuticle and tissue. Samples were processed using polymerase chain reaction (Nested-PCR) test. The results showed that 24% of the samples examined were clinically positive having white spots on their cuticles. Also, 92% of the samples examined were positive in PCR examination. Furthermore, 70% of apparently healthy shrimp were positively identified using the by PCR test. 3110 aquaculture Induction of tetraploidy in transgenic tilapia (Oreochromis niloticus) using physical shocks Farahmand H. Abdul Razak S.H. Hwang G.L. Maclean N. Rahman M.A Maclean N. 1 7 2007 7 1 27 46 09 12 2017 09 12 2017 The induction of tetraploidy by means of cold, heat and multiple heat shock treatments was investigated on male fish from a growth-enhanced transgenic tilapia C118 line, crossed with wild type females. After the development a new multiple heat shock protocol (two heat treatments at 41oC in 60 and 80 min. after fertilization for 5 min. per each), chromosome and X-gal in situ staining assays demonstrated the successful production of yolk sac tetraploid transgenic tilapia in two out of 15 embryos examined in this trial via direct correlation between higher level of b-galactosidase expression and karyotyping in the samples verifying complete tetraploidy in this line. In the grown fish, there was evidence to suggest mosaicism or perhaps tetraploidy in at least two grown fish using the measurement of erythrocytes and karyotyping of the G1 offspring (p<0.05). There was, however, no evidence to indicate successful production of triploid G1 individuals following crossing of induced fish with wild type. 3111 Stock assessment Poulations dynamics and stock assessment of common kilka (Clupeonella cultriventris caspia ) in Iranian waters of the Caspian Sea Fazli H. Zhang C.I. Hay D.E. Lee C.W. Janbaz A.A. Bourani M.S. 1 7 2007 7 1 47 70 09 12 2017 09 12 2017 This paper examines the changes in the population biology and biomass of common kilka (Clupeonella cultriventris caspia) in the Iranian waters of the Caspian Sea from 1995-2004. For most years during this 10-year period, we estimated the age structure of the catch, length-weight relationship, von Bertalanffy growth parameters, condition factor, sex ratios, maturity stages determined from ovarian analysis, natural and fishing mortality, age at first capture and biomass. Growth parameters were estimated as L¥=132mm, K=0.259/yr, t0=-1.285/yr. The instantaneous coefficient of natural mortality was estimated at 0.506/yr and the instantaneous coefficient of fishing mortality varied during the 10-year period between 0.125/yr to 1.487/yr. Biomass of the common kilka increased from about 16,000mt in 1995 to more than 41,000mt in year of 2002. This increase in common kilka was simultaneous with a sharp decline in anchovy kilka, changes in zooplankton abundance and composition, and especially increase in zooplankton species used by common kilka. We concluded that at the present time, the stock of common kilka is being over-fished. 3112 Biology & physiology Branchial chamber structure and osmoregulatory function in the prawn, Palaemon elegans (Crustacea: Decapoda) from the Caspian Sea Khodabandeh S. Golzari A. 1 7 2007 7 1 71 88 09 12 2017 09 12 2017 The structure, ultrastructure and osmoregulatory function of the branchial chamber were investigated in Palaemon elegans (Rathke, 1837) by light microscope, electron microscope and immunohistochemistry methods, respectively. Each chamber possesses a branchiostegite, 3 epipodites and 8 phyllobranchiate gills. The lamellae are attached along the two outer surfaces of the triangular gill axis. Ultrastructurally, numerous ionocytes are present along the innerside of the branchiostegites and in the epipodites. A consistently high immunofluorescence of Na+, K+-ATPase was observed along the basolateral sides of these ionocytes. The gill lamellae are formed by a single axial epithelium made of H-shaped cells with thin lateral expansions and a basal lamina limiting hemolymph lacunae. A positive but weak fluorescence of the Na+, K+-ATPase was found in lacunae sides of the lamellae cells. These findings show that in P. elegans, the epipodites and the branchiostegites organs appear as the main site of osmoregulation and the gill lamellae are mainly devoted to respiration. 3113 fish disease Myxobolus spp. (Myxosporea: Myxobolidea) from fishes of the Zayandeh-Rud River (Esfahan, Iran); new hosts and locality record Masoumian M. Barzegar M. Mehdipoor M. Asadollah S. Jalali B. 1 7 2007 7 1 89 100 09 12 2017 09 12 2017 In a parasitic survey of the native and introduced fishes in the Zayandehrud River, 5 Myxobolus spp. were found in the gills, fins and skin of examined fish specimens. They include M. varicorhini from fin and M. samgoricus from skin of Capoeta damascina, M. musajevi from gills of Capoeta capoeta gracilis, Myxobolus cristatus from Capoeta aculeata, and M. saidovi from Alburnus sp. Data on the location in the hosts comparing with previous records are provided. The epidemiological importance of Myxobolus spp. in Zayandeh-rud River is also presented. In the present paper the occurrence of five Myxobolus spp. all from endemic fishes from Zayandeh-rud River (A new locality) are recorded from two new hosts; Capoeta damascina, Capoeta aculeata. M. varicorhini and M. saidovi are recorded for the first time from Iranian freshwater fishes. 3114 Biology & physiology A Comparative SEM morphological study on the egg shell in some Anostracans (Crustacea: Branchiopoda) from East Azerbaijan Province of Iran Mehdizadeh Fanid L. Seidgar M. Azari Takami G. 1 7 2007 7 1 101 110 09 12 2017 09 12 2017 Abstract:  The surface morphology of resting eggs of Anostracan species from East Azerbaijan Province of Iran and that of Artemia urmiana was compared using Scanning Electron Microscopy (SEM). Anostracan fauna of East Azerbaijan temporary pools were determined as follows: Chirocephalus skorikowi (Chirocephalidae), Branchinecta orientalis (Branchinectidae), Streptocephalus torvicornis (Streptocephalidae), Branchinella spinosa (Thamnocephalidae) and Artemia urmiana (Artemiidae). However other species may also exist in these pools. Therefore, evaluating the morphology of these cysts by SEM appeared to accentuate that study of cyst ornamentation is a valuable taxonomical factor and more importantly that it can be used to identify the species even when the adults are not present at the time of sampling or in the absence of water. 3115 aquaculture The effects of extender type, freezing and thawing rates on fertility of the cryopreserved semen of the Caspian brown trout (Salmo trutta caspius ) Saravi Moghanloo M. Niksirat H. Mojazi Amiri B. Mirtorabi S.M. 1 7 2007 7 1 111 128 09 12 2017 09 12 2017 Cryopreservation of semen from the Caspian brown trout (Salmo trutta caspius) and effects of extender type, freezing and thawing rates on fertilization ability were studied. After assessment of semen quality, one part of semen was diluted with three parts of different extender and decanted into 0.5ml straws. Freezing was carried out at two freezing levels, 1.5cm and 2cm above surface of liquid nitrogen. The semen was thawed at 5°C for 90s, 15°C for 45s or 25°C for 30s in water baths and used for fertilization. Using the extender: 0.3 M glucose+10% methanol+10% egg yolk, and 0.6 M sucrose 10% DMSO + 10% egg yolk, yielded the highest post-thaw fertilization rates, with 67.05%±8.76 and 59.78%±5.08 eyeing rates, respectively. No significant differences were found in the fertilization rates with two freezing levels (P>0.05), however eyeing and hatching rates were higher for 2cm above the surface of the liquid nitrogen than for 1.5cm. Thawing of cryopreserved semen was best using the 25°C water bath for 30s and significant differences were seen in the eyeing rates between 25°C and 5°C or 15°C (P<0.05). Significant interactions (P<0.05) were found between effects of extender type and thawing rates and extender type and freezing rates. 3116 fish disease Vaccination of rainbow trout against Streptococcus iniae infection: comparison of different routes of administration and different vaccines Soltani M. Alishahi M. Mirzargar S. Nikbakht Gh. 1 7 2007 7 1 129 140 09 12 2017 09 12 2017 Antibody production and clinical efficacy (relative percent survival RPS) were measured in 40±5g rainbow trout after immunization with two types of Streptococcus iniae vaccines consisting of formalin killed cells (FKC) and FKC enriched with the bacterial extracellular products (ECP) administered by intraperitoneal (i.p), immersion and oral routes at 16±1°C for 18 weeks. No significant difference was found in antibody levels among the fish i.p immunized with FKC enriched with ECP plus Freunds' adjuvant (FA), FKC plus FA and FKC vaccines (P>0.05), whilst the antibody production was significantly higher in these three groups than fish immunized by immersion and oral routes of FKC and FKC enriched ECP (P<0.05). Also, fish immunized by immersion route with FKC showed significantly higher antibody titer than fish orally vaccinated with FKC with or without ECP until 12 weeks post-immunization (P<0.05). No significant difference was found in antibody titer between orally vaccinated fish and control groups (P>0.05). The RPSs ranging 82.6-100, 73.9-95 and 73.9-91.7% were obtained in the fish intraperitoneally immunized with FKC enriched ECP plus FA, FKC plus FA and FKC vaccines, respectively, compared to 0% survival for the control fish. Also, RPS in fish vaccinated by the immersion route was in the range 45.8-30.4% after 18 weeks post-vaccination. Efficacy of oral vaccination of fish with FKC plus ECP was in range of 8.7-29% and that of fish orally vaccinated with FKC resulted in 8.7-20.8% protection.