Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Effect of dietary A-Max supplementation as a prebiotic on growth performance and hemato- immunological parameters of great sturgeon (Huso huso Linnaeus, 1758) juveniles
251
266
FA
R.
Akrami
Department of Fisheries, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran.
akrami.aqua@gmail.com
Y
H.
Chitsaz
Department of Fisheries, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran.
N
F.
Lakzaei
Iranian Fisheries Sciences Research Institute,Tehran,Iran
N
An 8-week feeding trial was conducted to evaluate the effects of dietary commercial prebiotic A-Max, a mixture of mannan oligosaccharides, fructo oligosaccharides and β glucan, on growth and hemato-immunological parameters of great sturgeon (Huso huso) juveniles. After acclimation, fish (initial weight of 28.79±0.85 g) were placed into 12 tanks (30 fish per tank). Fish were fed a basal diet (containing 44% protein and 20% lipid) supplemented with 0 (control), 0.5, 1.0 and 1.5 g kg-1 A-Max in a totally randomized design in triplicate groups. At the end of the trial, growth factors and haemato-immunological parameters were assessed. Fish fed 1.5 g kg-1 prebiotic mixture displayed higher growth performances and feed efficiency compared to the control group (p>0.05). There were no significant differences in survival rate among all treatment groups (p>0.05). The results showed that RBC, WBC, lymphocyte, neutrophil, haematocrit and total protein were significantly affected by 1.5 g kg-1 dietary prebiotic mixture (p<0.05). An elevation of monocyte and haemoglobin (p>0.05) was found in the fish fed diet containing 1.0 g kg-1 prebiotic. In addition fish fed the diet with 1.5 g kg-1 prebiotic mixture showed a significant increase in lyzosyme activity, respiratory burst activity, serum total immunoglobulin (Ig) and alternative complement activity (ACH50) (p<0.05) compared with those fed the diets supplemented with other levels of prebiotics. The results showed that the addition of 1.5 g kg-1 prebiotic mixture to the diet of great sturgeon juveniles improving growth performance, some haematological parameters and immune response and seemed to be an effective immunostimulant.
Prebiotic mixture, Growth, Hematology, Immune response, Great sturgeon (Huso huso)
http://jifro.ir/article-1-3348-en.html
http://jifro.ir/article-1-3348-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
The relationships between heavy metals (As, Cd, Cu, Zn, Pb, Hg, Ni) levels and the size of pharaoh cuttlefish
(Sepia pharaonis) from Persian Gulf
267
287
FA
L.
Roomiani
Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
L.roomiani@iauahvaz.ac.ir
Y
Sh.
Jamili
Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran.
N
A.
Askary Sary
Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
N
S.
Ahmadi
Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
N
Concentrations of both essential (Zn and Cu) and non- essential (As, Cd, Pb, Hg, Ni) metals were measured in the organs (mantle, branchial hearts, gill and digestive gland) of Sepia pharaonis captured in the coastal waters of Bushehr Province in the Persian Gulf (Iran). The relationships between S. pharaonis size (mantle length) and metal concentrations in tissues were investigated by linear regression analysis. Metal concentrations (As µg g-1 d.w.) was highest in the digestive gland and lowest in the mantle of S. pharaonis, except for Ni. The concentrations of metals were found to follow the order: Zn> Cu>Pb> Ni>Hg> Cd> As. The mean Zn concentrations ranged from 37.72± 5.32 to 9.32± 3.73 µg g-1 d.w, whereas the mean As concentrations ranged from 0.01± 0.007 to 0.06± 0.05 µg g-1 d.w, respectively. Results of linear regression analysis showed that significant relationships between metal concentrations and fish size were positive, except for cadmium concentrations in the gills. The digestive gland of S. pharaonis would seem to constitute a good potential indicator of heavy metal concentrations in the marine environmental. Results from this study revealed that except for Pb the concentration of metals in S. pharaonis samples were below the threshold values As recommended by the FAO and EC guidelines.
Sepia pharaonis, Digestive gland, Heavy metals, Persian Gulf
http://jifro.ir/article-1-3349-en.html
http://jifro.ir/article-1-3349-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Reproductive biology of lotak, Cyprinion macrostomum Heckel, 1843 (Pisces: Cyprinidae), from
the Tigris River drainage
288
299
FA
H.
Faghani Langroudi
Department of Fisheries, Tonekabon Branch, Islamic Azad University, Mazandaran, Iran
hamid_faghani1@yahoo.com
Y
H.
Mousavi Sabet
Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, PO box: 1144, Guilan, Iran
N
Some reproduction aspects of the "doctor fish", Cyprinion macrostomum, were examined for the first time from the Tigris River drainage. A total of 316 specimens were caught from Gamasiab River in western Iran, at monthly intervals throughout the year from January to December 2013. Age, sex ratio, fecundity, oocytes diameter, gonado‑somatic (GSI), modified gonado‑somatic (MGSI) and Dobriyal (DI) indices were estimated. Regression analyses were used to find relations between fecundity and fish size (length and weight), gonad weight, and age. Sex ratio differed significantly from unity, and the percentage of males was greater than that of females. The mature females and males were longer than 108 mm and 94 mm in total length (+2 and +1 in age, respectively). The GSI ranged between 0.09-2.94% and 1.70-15.53% for males and females, respectively. Gonad indices showed spawning took place from May to August, when the water temperature was 16 to 24 °C. The average diameter of the oocytes ranged from 0.2 mm to 1.7 mm, in the spawning season. The averages of absolute and relative fecundity (relative to body weight) were 3642.51 eggs (SD ±1219.92) and of 55.04 (SD ±14.12) per gram body weight, respectively. The absolute fecundity was significantly related to body weight and ovary weight.
Fecundity, Maturity, Oocytes diameter, Gonado somatic, Iran
http://jifro.ir/article-1-3350-en.html
http://jifro.ir/article-1-3350-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Effects of selenium (Sel-Plex) supplement on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii)
300
312
FA
B.
Gholizadeh Zare Tavana
Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Alanbia University of Technology, Iran
N
M.
Banaee
Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Alanbia University of Technology, Iran
mahdibanaee@yahoo.com
N
A.
Yousefi Jourdehi
International Sturgeon Research Institution of Dr. Dadman, Rasht, Iran
Y
B.
Nematdoost Haghi
Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Alanbia University of Technology, Iran
N
M.H.
Seyed Hassani
International Sturgeon Research Institution of Dr. Dadman, Rasht, Iran
N
Selenium (Se) and many of its compounds are among the most toxic micronutrients. Therefore, determining the optimal level of selenium in the diet of fish is one of the main concerns of researchers. The present study was designed to investigate the effects of Sel-Plex® (Selenium-enriched Saccharomyces cerevisiae yeast) on blood biochemical parameters in juvenile Siberian sturgeon, Acipenser baerii, as clinical biomarkers to evaluate the effects of selenium on the health of fish. In this study, fish were fed diets supplemented with 0 (control), 5, 10 and 15 g Sel-Plex per Kg feed for 8 weeks. Although a significant (p<0.05) decrease was observed in alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT) activity in fish fed with 15 g Sel-Plex supplement, plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were significantly higher (p<0.05) than the control fish. The results showed that administration of 10 and 15 g Sel-Plex supplement in fish significantly (p<0.05) increased lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) activities. Administration of Sel-Plex significantly (p<0.05) decreased glucose, albumin, cholesterol and triglyceride concentrations. The results revealed that Sel-Plex significantly increased (p<0.05) globulin concentrations in the supplemented groups compared with the control group. Decreased total protein concentrations were only observed in fish fed with 5 g Sel-Plex. The results of this study show that diets containing high concentrations of Sel-Plex (10 g and 15 g) produced serious toxic effects, including changes in blood biochemical parameters.
Siberian sturgeon, Biochemical parameters, Selenomethionine, Sel-Plex supplement
http://jifro.ir/article-1-3351-en.html
http://jifro.ir/article-1-3351-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Growth, mortality and exploitation of buccaneer anchovy (Encrasicholina punctifer Fowler, 1938) from the north Persian Gulf and Oman Sea
313
326
FA
A.
Salarpouri
Department of Fisheries, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran. P.O. Box: 3955
N
E.
Kamrani
Department of Fisheries, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran. P.O. Box: 3955
ehsan.kamrani@hormozgan.ac.ir
Y
F.
Kaymaram
Fisheries Science Research Institute, Tehran, Agricultural Research, Education and Extension Organization, Iran. P.O.Box: 149-14965
N
R.
Mahdavi Najafabadi
Department of Natural Resources Engineering, Hormozgan University, Bandar Abbas, Iran. P.O.Box: 3955
N
The population parameters of Encrasicholina punctifer (buccaneer anchovy) from commercial fisheries in the Persian Gulf and Oman Sea coastal waters were studied from October 2014 to September 2015. A total of 13951 length-frequency data were collected monthly by pair-boat purse seine and beach seine methods. The asymptotic length (L∞) and Von Bertalanffy growth function (K) were 11.5 cm and K=1.4 per year, respectively. The value of to was calculated as -0.14 year. Longevity was estimated as 2 years. The total length ranged from 2.9 to 10.1 cm, with an average of 6.5 cm. The probability of capture was calculated in total length as L25=6.05 cm, L50=6.48 cm and L75=6.93 cm. Four cohorts were distinguished annually with mean lengths of 4.56, 5.77, 7.21 and 8.50 cm. The length-weight relationship was determined as W= 0.0051 L 3.1114. Total, natural and fishing mortality parameters were estimated as 6.15, 2.84 and 3.32 yr-1 respectively. The current rate of exploitation (E) was 0.54, and the values of exploitation ratio was found reasonable for current fishing effort. From the yield-per-recruit analysis, Emax, was estimated as 0.91. The results indicated that the buccaneer anchovy stock was not overexploited. Since E<emax, it confirmed the good potential of anchovy stock in the area.
</e
Encrasicholina punctifer, Growth, Mortality, Persian Gulf, Oman Sea
http://jifro.ir/article-1-3352-en.html
http://jifro.ir/article-1-3352-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
An investigation on the use of Prosopis juliflora pods as a carbohydrate source supplemented with probiotics in the diet of Labeo rohita fingerlings
327
345
FA
S.
Chovatiya
Ashok & Rita Patel Institute of Integrated Study & Research in Biotechnology & Allied Sciences, New Vallabh Vidyanagar-388 121 Gujarat, India.
N
S.
Bhatt
B R Doshi School of Biosciences, Sardar Patel University, Vallabh Vidyanagar–388 120 Gujarat, India.
bhatt.sujata@gmail.com
Y
A.
Shah
B R Doshi School of Biosciences, Sardar Patel University, Vallabh Vidyanagar–388 120 Gujarat, India.
N
P.
Dube
B R Doshi School of Biosciences, Sardar Patel University, Vallabh Vidyanagar–388 120 Gujarat, India.
N
The present study investigated the use of fermented Prosopis juliflora pods (PJP) as a carbohydrate source alternative to rice bran (RB), and a P. juliflora pods based diet formulation supplemented with probiotics Lactobacillus acidophilus (LA), Lactobacillus bulgaricus 009 (LB) and Streptococcus thermophilus (ST) on the growth of Labeorohita (rohu) fingerlings. The diets were isonitrogenous (39%) and isoenergetic (15.9 KJg-1 gross energy); and were prepared using rice bran (as reference diet RD), crude pods (PJPC), fermented pods (PJPF) and with probiotics L. acidophilus (PJPLA), L. bulgaricus (PJPLB) and S. thermophilus (PJPST). The results showed that fermentation of P. juliflora pods with L. acidophilus significantly increased (p<0.05) protein, lipid, Ca, Fe, Mg, K and reducing sugar; and completely eliminated phytic acid. The diets PJPF and PJPLA produced significantly higher (p<0.05) percent weight gain, SGR, FCR and PER as compared to PJPC, PJPLB and PJPST diets; and achieved growth performance comparable to RD. An improvement (p<0.05) in the carcass protein has been detected in groups PJPF and PJPLA in comparison to RD, PJPC, PJPLB and PJPST. The L. acidophilus was found to be effective in controlling coliform count in gut as compared to L. bulgaricus and S. thermophilus; and also in controlling total viable count, total coliform count and fecal streptococci count in fecal matter. The results indicate that PJPF and PJPLA deserve further investigations as alternative feed for rohu.
Prosopi sjuliflora pods, Antinutritional factors, Probiotics, Fermentation, Growth, Microflora.
http://jifro.ir/article-1-3353-en.html
http://jifro.ir/article-1-3353-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Pysiological effects of NaCl on Ceratophyllum demersum L., a submerged rootless aquatic macrophyte
346
356
FA
M.
Dogan
Department of Biology, Faculty of Arts and Sciences, University of Gaziantep, 27310 Gaziantep, Turkey
doganm@gantep.edu.tr
Y
S.
Demirors Saygideger
Department of Biology, Faculty of Arts and Sciences, University of Gaziantep, 27310 Gaziantep, Turkey
N
In the present study, effects of NaCl concentrations (0, 20, 40 and 80 mM) were investigated on Ceratophyllum demersum. Macronutrients N, P and K and micronutrients Zn and Cu were significantly decreased by NaCl salinity. In general, however, Fe and Mn contents of macrophyte tissues increased. As a result, NaCl toxicity disturbed the uptake and translocation of macro and micronutrients in the macrophyte and induced its nutrient imbalance. Although proline, ascorbate and non-protein protein thiols amounts were increased with salinity, contents of photosynthetic pigments, total soluble carbohydrate, reducing sugar and protein were reduced. Observed increases in malondialdehyde contents, one of the most frequently used indicators of lipid peroxidation, showed that oxidative damage occurred in membranes resulting from NaCl. High salinity stress developed some toxicity symptoms. These symptoms probably occurred due to ionic toxicity, imbalance of macro and micronutrients and/or oxidative stress triggered by salinity.
Ceratophyllum demersum, NaCl, Physiological effects, Morphological effects
http://jifro.ir/article-1-3354-en.html
http://jifro.ir/article-1-3354-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Population dynamics of Carasobarbus luteus in Shadegan International Wetland, Iran
357
368
FA
R.
Ghorbani
Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
rasulghorbani@gmail.com
Y
A.
Hedayati
Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
N
A.R.
Hashemi
Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
N
M.H.
Abolhasani
Department of Natural Resources, Faculty of Agriculture, Islamic Azad University, Khorasgan, Isfahan, Iran.
N
A.
Haghi Vayeghan
Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
N
M.
Amini Charmahini
Behbahan Khatam Alanbia University of Technology, Khoozestan, Iran
N
Seasonal samplings were conducted over a year to study population dynamic of Carasobarbus luteus in Shadegan Wetland. Samples were collected from five sampling stations in Shadegan Wetland (Salmane, Rogabe, Khoroosi, Mahshahr and Atish) from April 2014 to March 2015. From total number of 1608 samples, 276 individuals were male and 1332 individuals were female. Length-weight relationship was W=0.013L3.0124 (R2=0.92) for males; and W=0.0136L3.0117 (R2=0.91) for females, showing an isometric growth pattern. Condition factor (K) was calculated to be 1.308±0.15 and 1.368±0.17 for the males and females, respectively, showing significant difference between the genders (p<0.05). Also, the results show that this species spawns in April-July with a long spawning period and sexual dormant period in autumn to early winter. Mean length at maturation was 161 and 174 mm for males and females specimens; whereas, weight of matured fish was 60 and 80 g for the males and females, respectively. Growth indices including L∞, growth coefficient and t0 were 305 and 301 mm, 0.67 and 0.55 per year and 0.23 and 0.29 for the males and females, respectively. Natural mortality, fishing mortality, total mortality, Munro Ф' and coefficient of exploitation were 1.22 and 1.02 per year, 1.61 and 1.45 per year, 2.83 and 2.47 per year, 2.79 and 2.72, and 0.57 and.59, respectively for the males and females.
Carasobarbus luteus, Shadegan Wetland, Growth and fishing indices, Von-Bertalanffy equation
http://jifro.ir/article-1-3355-en.html
http://jifro.ir/article-1-3355-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Isolation and long-term culture of neural stem cells from
Acipenser persicus (Borodin, 1897)
369
380
FA
H.
Kashiri
Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
N
A.
Shabani
Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
ali_shbany@yahoo.com
Y
K.
Haidari
Department of Anatomy, Golestan University of Medical Sciences, Gorgan, Iran
N
M.R.
Imanpour
Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
N
M.
Saeedi
Department of Microbiology and Immunology, University of Medical Sciences, Gorgan, Iran
N
In the present study, an in vitro brain cell culture was developed from neural cells of Persian sturgeon (Acipenser persicus). The tissue samples collected from the anterior, middle and posterior regions of the brain were cultivated separately in DMEM/F12 medium supplemented with 15% fetal bovine serum, antibiotic and antimycotic. The medium was refreshed every 3 days. The cells became confluent after about 3 weeks from the initial time of seeding. The cultured cells from the posterior part of the brain showed high potential of proliferation as they had been passaged 16 times in more than 11 months. To determine optimal temperature, the brain cells were incubated at four temperatures including; 20, 22, 25 and 28°C. The best cultivation temperature was obtained at 25°C. The cultured cells from posterior part of the brain were cryopreserved successfully and the survival rate was 70% after thawing. Immunocytochemistry using antibody against nesting showed that some cells were immunopositive for nesting. Finally, these results suggested that cell cultures from posterior part of the Persian sturgeon brain with high proliferation capacity can be useful for research on brain cells in A. persicus in the future.
Brain, Cell culture, Immunocytochemistry, Persian sturgeon (Acipenser persicus)
http://jifro.ir/article-1-3356-en.html
http://jifro.ir/article-1-3356-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Effects of Arthrospira platensis on growth, skin color and digestive enzymes of Koi, Cyprinus carpio
381
393
FA
F.
Ansarifard
Department of Fisheries, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775,Tehran, Iran.
fansarifard@yahoo.com
N
H.
Rajabi Islami
Department of Fisheries, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775,Tehran, Iran.
rajabi.h@srbiau.ac.ir
Y
M.
Shamsaie Mehrjan
Department of Fisheries, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775,Tehran, Iran.
N
M.
Soltani
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
N
This study evaluated the effects of diets containing 0, 2.5, 5, 7.5 and 10% Arthrospira platensis on skin pigmentation, growth performance, and digestive enzymes of koi, Cyprinus carpio. A completely randomized design was performed with five treatments and three replicates. One hundred and fifty koi fish with average initial weight of 30±1g were assigned to fifteen experimental tanks. The experiment lasted for eight weeks. Koi fish fed with 2.5% A. platensis (1.17±0.02) showed a significant lower growth performance than those fed with 7.5 and 10% A. platensis (1.39±0.02 and 1.46) (p<0.05). However, carotenoid (astaxanthin) concentrations of skin significantly increased with increased levels of dietary algae (p<0.05). The highest values of a* (red zones) and b* (yellow zones) were observed in fish skin fed with 10% A. platensis (4.33±0.15, 5.23±0.15, 5.23±0.15, 5.7±0.36, and 6.33±0.32, respectively). The activities of protease, amylase and lipase in dietary supplementation of 10%A. platensis were significantly higher than those of the control group (protease from 5.17±0.76 to 11 , amylase from 22±1.73 to 32.67±1.53 and lipase from 16.33±2.08 to 74.33±1.53. Liver enzymes also decreased significantly by increased dietary supplementation. (ALT: from 192.33±5.5 to 80.67±1.52, AST: from 1741.7±18.92 to 712.33±10.5, ALP: from 452.33±8.14 to 48±1, LDH: from 7287.3±34.64 to 7119.7±17.89). These results revealed that inclusion of 5-10% A. platensis in diets has a significant positive effect on growth rate, pigmentation, and improvement of digestive and liver enzymes activities in koi fish.
Arthrospira platensis, Pigmentation, Growth, Carotenoids, Enzymes activities, Koi
http://jifro.ir/article-1-3357-en.html
http://jifro.ir/article-1-3357-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Effects of dietary supplementation of date palm (Phoenix dactylifera) seed extract on body composition, lipid peroxidation and tissue quality of common carp (Cyprinus carpio) juveniles based on the total volatile nitrogen test
394
402
FA
M.
Mohammadi
Department of Aquaculture, Science and Research Branch, Islamic Azad University, Tehran, Iran
m40_mohammadi@yahoo.com
Y
M.
Soltani
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
N
A.
Siahpoosh
Medicinal Plants Research Center and Department of Pharmacognosy, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
N
M.
Shamsaie
Department of Aquaculture, Science and Research Branch, Islamic Azad University, Tehran, Iran
N
Date palm seed (Phoenix dactylifera) (DPS) extract has been used worldwide in pharmaceutical agent due to its constituents. This study aimed to assess the effects of DPS extract as a dietary supplementation at on the body composition, lipid peroxidation and tissue quality of common carp (Cyprinus carpio) based on the total volatile nitrogen (TVN) test. Common carp juveniles weighing ca 32 g were cultured in 26°C freshwater for 60 days and fed with diets supplemented with 0.5%, 1%, 2% and 4% of DPS extract. At the end of the trail, the crude protein (p>0.05) and crude lipid (p<0.05) were higher in fishes fed with diet containing 0.5% DPSthan control group, while their ash (p<0.05) and moisture content were lower in than other groups (p>0.05). The increase in DPS ratio of diet above 0.5% , resulted in lower crude protein and crude lipid, while the ash and moisture content increased. In addition, the values of TVN and lipid peroxidation in muscle and brain were lower in all treatments fed with supplemented diet compared to the control group. The results indicated that utilization of 0.5% DPS in commercial diet of carp can improve the body composition and antioxidant defense system of fish.
Date seed extract, Body composition, Lipid peroxidation, TVN, Carp
http://jifro.ir/article-1-3358-en.html
http://jifro.ir/article-1-3358-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Comparison of dietary butyric acid supplementation effect on growth performance and body composition of
Clarias gariepinus and Oreochromis niloticus fingerlings
403
412
FA
O.O.
Omosowone
Department of Fisheries and Aquaculture Technology, Federal University of Technology, Akure, Ondo State, Nigeria.
N
A.A.
Dada
Department of Fisheries and Aquaculture Technology, Federal University of Technology, Akure, Ondo State, Nigeria.
aadada@futa.edu.ng
Y
E.O.
Adeparusi
Department of Fisheries and Aquaculture Technology, Federal University of Technology, Akure, Ondo State, Nigeria.
N
This study was conducted using butyric acid as a growth enhancer in the diets of Clarias gariepinus and Oreochromis niloticus. C. gariepinus (42.39 g±1.17) were fed diets containing varying inclusion levels of butyric acid (BA); 0 (CG1), 0.5 (CG2), 1.0 (CG3), 1.5 (CG4), 2.0 (CG5) g kg -1, where, CG1 is the control or reference diet. O. niloticus (25.50 g±0.50) were also fed diets containing varying inclusion levels of butyric acid; 0 (ON1), 0.5 (ON2), 1.0 (ON3), 1.5 (ON4), 2.0 (ON5) g kg -1, where, ON1 is the control or reference diet. Diets for C. gariepinus contained 40 % while those of O. niloticus had 35 % crude protein. Fish were fed to satiation twice daily in two equal portions for 12 weeks. At the end of the experiment C. gariepinus fed on diet CG5 (2 g kg-1 BA) had an improved growth of over 600 % while O. niloticus fed diet ON4 (1.5 g kg-1 BA) showed over 400 % improvement in growth when compared with those fed the control or reference diets. The highest carcass protein were also obtained in those fed BA diets when compared with their respective control diets. The results of the present study showed that there was better nutrient utilization in C. gariepinus and O. niloticus fed BA supplemented diets. Furthermore, there were no significant variations in the survival of fish fed BA diets when compared with their controls except for those fed ON4 which showed 100 % survival. Quality of water parameters were within the acceptable limits for the culture of fin fishes.
Butyric acid, Growth enhancer, Clarias gariepinus, Oreochromis niloticus, Survival
http://jifro.ir/article-1-3359-en.html
http://jifro.ir/article-1-3359-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Antioxidant and cytotoxic activities of metabolites produced by a new marine Streptomyces sp. isolated from the sea cucumber Holothuria leucospilota
413
426
FA
M.
Gozari
Department of Microbiology, College of Science, Science and Research Branch, Islamic Azad University, Fars, Iran
N
N.
Bahador
Department of Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
bahador@iaushiraz.ac.ir
Y
A.R.
Jassbi
Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
N
M.S.
Mortazavi
Persian Gulf and Oman Sea Ecological Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Bandar Abbas, Tehran, Iran.
N
E.
Eftekhar
Molecular Medicine Research Center, Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
N
Marine microorganisms are important sources for novel natural products. Hence, the aim of this study was to introduce marine microorganisms with the capability of producing antioxidant and cytotoxic metabolites. For this purpose, ten live sea cucumbers (Holothuria leucospilota) were collected from Larak Island, Persian Gulf. Then, their intestine contents were serially diluted and treated by heating (55°C). 100 µL of treated samples were inoculated on starch casein nitrate agar medium, which is supplemented with nalidixic acid and cycloheximide. The plates were incubated at 28 °C for 4 weeks and the colonies were purified. The antioxidant activity of extracted metabolites from the isolated actinobacteria was evaluated using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity assay and the cytotoxic activity was screened by Brine-Shrimp micro well cytotoxicity method. In addition, the cytotoxic effect was evaluated against HCT 116 and SW 480 cell lines by MTT cell proliferation assay. A new strain of marine actinobacterium represented maximum antioxidant and cytotoxic activity among 17 actinobacterial isolates. The ethyl acetate culture extract of the isolate scavenged DPPH radicals with IC50 value of 211.2 µg mL-1. In addition, the extract exhibited high toxicity against HCT 116 and SW 480 tumor cell lines with IC50 values of 26.48 and 18.53 µg mL-1 respectively. The isolate was identified as Streptomyces sp. strain SC 156 and showed 98% similarity with type strains in NCBI database. These results suggested that Streptomyces sp. strain SC 156 could be considered as promising candidate for antioxidant and cytotoxic compound discovery.
Antioxidant activity, Cytotoxic activity, Holothuria leucospilota, Marine Streptomyces
http://jifro.ir/article-1-3360-en.html
http://jifro.ir/article-1-3360-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Short communication:GIS based hydro-biological parameter approach for identification of productive zones in Nanak Sagar Reservoir of Uttarakhand, India
427
434
FA
N.A.
Ingole
Directorate of Coldwater Fisheries Research, (ICAR), Bhimtal- 263136, Uttarakhand, India
nanduingole122@gmail.com
Y
A.S.
Nain
G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, Uttarakhand, India
N
R.H.
Rathod
College of Fishery Science, MAFSU, Nagpur, Maharashtra, India
N
R.
Posti
Directorate of Coldwater Fisheries Research, (ICAR), Bhimtal- 263136, Uttarakhand, India
N
R.
Chalal
Directorate of Coldwater Fisheries Research, (ICAR), Bhimtal- 263136, Uttarakhand, India
N
Reservoirs constitute the single largest inland fishery resource, both in terms of size and productive potential. The productivity of a reservoir depends on various factors such as the available water area, water quality, availability of planktonic food, stocking rate, methods of exploitation of the stock and the type of management of the fishery (Vass and Sugunan, 2009). A desirable level of productivity can be achieved in this man-made ecosystem only by a proper control of the above-mentioned factors on scientific lines. Geographical Information System (GIS) and Remote Sensing and mapping technologies can be used in selecting sites for culture and capture fisheries (Kapetsky, 1987). The maps can provide a long-term outline to the planners for aquaculture development (Kapetsky, 1989).
Hydro-biological parameters, Productive zones, Reservoir fisheries, GIS model
http://jifro.ir/article-1-3361-en.html
http://jifro.ir/article-1-3361-en.pdf
Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences
1562-2916
17
2
2018
4
1
Short communication:Potential ectoparasites of the endemic Mediterranean banded killifish (Aphanius fasciatus, Valenciennes, 1821) of the northern Sahara (Algeria)
435
442
FA
C.
Ghazi
Department of Biology, Faculty of Nature and Life Sciences; and Sciences of the Earth and the Universe. University of Kasdi Merbah, Ouargla, Algeria.
ch-gh-eco@hotmail.com
Y
A.
Si Bachir
Department of Ecology and Environment, Faculty of Nature and Life Sciences, University Batna 2, Batna, Algeria.
N
F.
Santoul
EcoLab, Laboratoire d’Ecologie Fonctionnelle, University Toulouse III, France.
N
R.
Céréghino
EcoLab, Laboratoire d’Ecologie Fonctionnelle, University Toulouse III, France.
N
The Aphanius fasciatus is a euryhaline and eurythermal fish, naturally present in many brackish water habitats little oxygen, along the Mediterranean coast, rarely in freshwater (Maltagliati, 1999). The species is endemic to the Mediterranean area, where it is found almost all coasts. Recently, Chaibi et al., (2015), reported the presence of the species in the northern part of the northern Sahara region, which widens the biogeographic area known distribution of the species, which was previously limited to coastal areas around the Mediterranean to continental habitats, including brackish water systems in the desert region of Algeria.
Aphanius fasciatus, Ectoparasites, Population dynamics, Sahara Algeria.
http://jifro.ir/article-1-3362-en.html
http://jifro.ir/article-1-3362-en.pdf