OTHERS_CITABLE
Evaluation of LHRH-a acute release implantation on final maturation and spawning in not-fully matured broodstocks of Persian sturgeon (Acipenser persicus Borodin, 1897)
The Persian sturgeon (Acipenser persicus) is considered as an endemic sturgeon of the
south part of the Caspian Sea and provides the highest Iranian caviar
production. Due to overfishing, degradation of the rivers conditions of the
natural reproductive habitats, the fish stocks is decreasing. The immature
breeders do not response to hormonal therapy at the sturgeon hatcheries as most
having PI (above 10) and large numbers of breeders caught and transported to
the hatcheries were unable to reproduce. This study was attempted to find the
effect of LHRHa implantation on oocytes maturation and spawning of Persian
sturgeon. Broodstocks were caught from the southeast region of the Caspian Sea.
The selected female broodstocks (PI>10) ranged in size from 24.0 to 37.5 kg
were implanted with LHRHa cholesterol pellets at concentrations of 0 μg/kg (control),
and treatments of 10, 15 and 20 μg/ kg (in
three replicates. The results from this study indicated that females treated with LHRHa
hormone implantation at 10, 15, 20 µg per kg body weight reached final maturation.
These results were observed for all fish from treatment numbers 2 (15 μg/ kg) and 3
(20 μg/kg), however only one fish reached final maturation in treatment 1(10 μg/ kg). The
current implantation of LHRHa was able to enhance breeders with PI above 10 to
final maturation which under normal condition at sturgeon’s hatcheries this is
not possible. The results suggested that final maturation can be achieved that
lead to high fertilization (78.33% ±13.87, 68.33% ±4.16 in treatment 2 and 3, respectively) and hatching rates (85.3% ±9.07, 68.33% ±7.64 in treatment 2 and 3, respectively) and also total larvae production. Thus, the information from this
study is very useful for artificial propagation of not-fully-matured females of
Persian sturgeon at Sturgeon hatcheries especially in Iran.
http://jifro.ir/article-1-619-en.pdf
2015-09-13
440
459
Acipenser persicus
Broodstocks
LHRHa implantation
Artificial propagation
Fertilization
Hatching rates
K.
Amini
kourosh_a_ca@yahoo.com
1
AUTHOR
S. S.
Siraj
2
AUTHOR
B.
Mojazi Amiri
3
AUTHOR
S. A.
Mirhashemi Rostami
4
AUTHOR
A.
Sharr
5
AUTHOR
H.
Hossienzadeh
6
AUTHOR
OTHERS_CITABLE
Benthic invertebrate community in Khur-e-Mussa creeks in northwest of Persian Gulf and the application of the AMBI (AZTI\'s Marine Biotic Index)
Benthic invertebrates are a
well-established target in environmental quality status evaluations. The AMBI
(AZTI's Marine Biotic Index) was developed to determine the impacts and the
quality status of soft-bottom marine benthic communities. The aim of this study was investigating multivariate
pattern of benthic invertebrate community and application of AMBI (AZTI's
Marine Biotic Index) for determining quality of health status in Khuzestan
coastal waters. Seasonal samplings
were collected from eight creeks over one year study by Peterson grab sampler,
beginning from October 2005. All
creeks are characterized by muddy bottom. According
to sensitivity to environmental stress, macrobenthic
animals classified into 5 ecological
groups. Among 28
identified faunal groups the most abundant groups were polychaets, molluscs, crustaceans and nematodes, respectively. Two way ANOVAs showed
only seasonal significant differences in mean abundance (P<0.05) and Margalef
richness index values (P<0.05), while all of studied creeks shows similar
biological characters based on benthic communities. According
to annual mean of AMBI value all creeks were in ecological group III with
slightly polluted condition, except for Darvish that was in unpolluted category. In the present study, although the number of
species initially increased, but due to appearance of dominant species such as Capitella
sp. and nematods (as opportunist species), diversity values reduced.
In general, according to AMBI and Biotic index values, the most creeks are
classified into unpolluted and slightly polluted categories except for Zangy,
Doragh and Patil in summer and also Zangy and Bihad in winter which was in
moderate to heavily polluted category. Different types of exploitation, industrial and shipping activities in this area could result in unbalanced
to polluted status in benthic animals (as the best indicators in sediment
quality assessment) so these results can be
acceptable.
http://jifro.ir/article-1-620-en.pdf
2015-09-13
460
474
AMBI
Contaminant assessment
Khur-e-Mussa creeks
Persian Gulf
S.
Dehghan Madiseh
s_dehghan2002@yahoo.com
1
AUTHOR
F.
Esmaily
2
AUTHOR
J. G.
Marammazi
3
AUTHOR
E.
Koochaknejad
4
AUTHOR
S.
Farokhimoghadam
5
AUTHOR
OTHERS_CITABLE
The relation between environmental parameters of Hormuzgan coastline in Persian Gulf and occurrence of the first harmful algal bloom of Cochlodinium polykrikoides (Gymnodiniaceae)
Cochlodinium polykrikoides was
bloomed in the Persian Gulf for the first time in September 2008, started from
the Strait of Hormuz and then spread out towards the northern parts covering
most of the areas of the Gulf and lasted 8 months. In order to find out
environmental conditions during the bloom, a monthly sampling program was
carried out in seven surface stations in 2008-2009. At each station, three
samples (triplicates) were collected for phytoplankton analysis and also one
sample for environmental analyses, including salinity, chlorophyll a and nutrients.
Blooms of C. polykrikoides were observed with a
seawater temperature of 20.1 to 31.0 °C,
salinity 37.0-40.1 ppt and nutrient concentration ranges during the bloom and red tide were 0.064-0.707 mg/l nitrate + nitrite
and 0.001-1.66 mg/l phosphate, respectively. Maximum of C. polykrikoides abundance was measured 26 × 106 cells L-1 in
October 2008. Kruscal wallis test demonstrated a significant difference
in densities and chlorophyll-a in different months and seasons not in different
stations. This study showed increase of temperature (>31.0 °C) stopped bloom and red tide due to C.
polykrikoides in both the eastern and middle sampling stations but in the
western stations was determined decrease in nutrient amounts as the major
factor therefore increased nutrient of coastal waters, and environmental
conditions could have efficacy the occurrence of this dinoflagellate.
http://jifro.ir/article-1-621-en.pdf
2015-09-13
475
489
Red tide
Cochlodinium polykrikoides
Environmental conditions
Nutrients
Persian Gulf
S. M. R.
Fatemi
1
AUTHOR
S. M. B.
Nabavi
2
AUTHOR
G.
Vosoghi
3
AUTHOR
M.
Fallahi
4
AUTHOR
M.
Mohammadi
mojhde.bam52@yahoo.com
5
AUTHOR
OTHERS_CITABLE
Effects of Propolis on microbiologic and biochemical parameters of Rainbow trout (Oncorhynchus mykiss) after exposure to the pesticide
Cypermethrin is a
potential toxic pollutant that directly threatens the aquatic ecosystems and
environment. Propolis, a natural product collected from plants by honey bees
has many functions, such as antimicrobial, antioxidant, and anti-inflammatory.
This study attempted to detrmine some biochemical and microbiological
parameters of rainbow trout fillets after exposure to propolis
and cypermethrin. The results of the current study revealed that levels of
malondialdehyde (MDA), lactic acid, total volatile base-nitrogen (TVB-N), total
counts of psychrophilic, and mesophilic bacteria increased in cypermethrin
groups (P<0.05), compared to control group. Furthermore, there were no
significant differences in pH levels between groups. Additionally, levels of
MDA, lactic acid, TVB-N, count of psychrophilic, and mesophilic bacteria in
cypermethrin+propolis treated groups were significantly reduce in comparison to
exposed groups with cypermethrin (P<0.05). The findings also showed that the
fillet quality some biochemical and microbiologic functions of fishes could be changed
in groups that exposed to cypermethrin by propolis.
http://jifro.ir/article-1-622-en.pdf
2015-09-13
490
503
Biochemical parameter
Cypermethrin
Microbiological parameter
Propolis
Oncorhynchus mykiss
M.
Fuat Gulhan
1
AUTHOR
A.
Duran
2
AUTHOR
Z.
Selamoglu Talas
ztalas@nigde.edu.tr
3
AUTHOR
S.
Kakoolaki
4
AUTHOR
S. M.
Mansouri
5
AUTHOR
OTHERS_CITABLE
Effect of solvent extracted soybean meal and full-fat soya on the protein and amino acid digestibility and body amino acid composition in rainbow trout (Oncorhynchus mykiss)
This study was carried out to investigate the apparent digestibility
coefficients (ADCs) value of protein, amino acid and energy and body amino acid
composition of rainbow trout fed
solvent extracted soybean meal (SBM) and full-fat soybean meal (FFS) partly
replacing fish meal (FM) in diets. Five iso nitrogenous (average 50.36% crude
protein) and energetic (4294 kcal/kg total energy) diets were designed in 5
groups in triplicates. Diet one as control group with 43% FM and 25% SBM, Diet
2 with 28% FM and 40% SBM, Diet 3 with 13% FM and 55% SBM, Diet 4 with 28% FM
and 40% FFS, Diet 5 with 13% FM and 55% FFS. Diets were fed to trout fishes with
49 g initial
body weight for 20 weeks at 8.8±0.45 ºC. It was found that ADCs of energy,
protein and most amino acids particularly methionine and lysine and fish body
muscle lysine levels, significantly decreased by increasing the amount of FFS
and SBM in diets (P<0.05). On the other hand, valine, isoleucine, threonine,
methionine, phenylalanine, lysine and histidine showed a decreasing tendency
with the increasing proportion of SBM in fish body composition. It was concluded
that increasing the SBM and FFS percent replacing FM in trout fish diet, caused
reducing protein, amino acid and energy digestibility, compared with the
control diet. However, it can also be concluded that the replacement of SBM was
more efficient than the replacement of FFS as regarding growth, ADCs of protein
and essential amino acid contents of O. mykiss.
http://jifro.ir/article-1-623-en.pdf
2015-09-13
504
517
Rainbow trout
Oncorhynchus mykiss
Soybean meal
Full-fat soybean meal
Amino acid
Digestibility
A. G.
Harlıoğlu
aharlioglu@firat.edu.tr
1
AUTHOR
OTHERS_CITABLE
Effects of Soy protein concentrate and Xanthan gum on physical properties of Silver carp (Hypophthalmichthys molitrix ) surimi
Studies
were conducted to evaluate the effects of soy protein concentrate (SPC) and
Xanthan gum (X) on physical properties of surimi prepared from Silver carp (Hypophthalmichthys molitrix)
using various amount of soy protein concentrate (0, 5 and 10%) and xanthan gum
(0, 0.25 and 0.5%) in fish paste during 3 months of frozen storage at -18ºC.
Results obtained from folding test showed that the gel-forming ability of the
gel treated with commercial blend (4% sorbitol+4% sucrose) was higher than the
gels containing soy protein concentrate and xanthan gum (P< 0.05). Xanthan
gum had a harmful effect on gel formation of surimi. The lowest expressible
moisture values were reported for the gels containing 10% SPC and commercial
blend (4% sorbitol+4% sucrose) that indicated the gels matrix had high water
holding capacity (P< 0.05). During 3 months of frozen storage at -18ºC,
gel-forming ability and water holding capacity of surimi were markedly decreased
(P<0.05). Results of this studies demonstrated that the samples consisting
commercial blend (4% sorbitol+4% sucrose) and 10 % SPC were more acceptable
from the viewpoint of water holding capacity and gel forming ability,
respectively.
http://jifro.ir/article-1-624-en.pdf
2015-09-13
518
530
Surimi
Soy protein concentrate
Xanthan gum
Gel-forming ability
Expressible moisture
F.
Hasanpour
1
AUTHOR
E.
Hoseini
ebhoseini@srbiau.ac.ir
2
AUTHOR
A. A.
Motalebi
3
AUTHOR
F.
Darvish
4
AUTHOR
OTHERS_CITABLE
Effects of different levels of energy and protein sources on the growth performance, feeding, survival rate and the chemical body composition of juvenile pacific white shrimp
The present study was carried out in order to
establish an economical effective diet for the pacific white shrimp in the southern
part conditions of Iran. Three dietary energy levels (E1=262, E2=312, E3=362
Kcal 100 g-1 diet) and 6 ratios of fish meal (FM): soybean meal (SBM)
[(P1= 100% FM+ 0% SBM), (P2= 80% FM+ 20% SBM),(P3= 60% FM+ 40% SBM), (P4= 40%
FM+ 60% SBM), (P5= 20% FM+ 80% SBM),(P6= 0% FM+ 100% SBM)], 18 experimental
diets were prepared. Completely randomized design was used to assign 54
polyethylene 300 litre round tanks provided by aeration and was stocked by 19
juvenile shrimp as 3 replicates to each treatment. Shrimps average weight was
about 0.77 g at the start. After 56 days culture period, maximum growth and
nutritional performance were observed in the P6E1 and P5E1 treatments. In
addition, the highest survival rate of the shrimps was observed in the P1E1,
P1E2, P3E3 and P5E3 treatments. Results indicated that protein, fat, fiber and
ash contents of carcase were significantly affected by the treatments
(P<0.05). Results of the present study suggest the replacement possibility
of at least 80% of dietary fishmeal by soybean meal in the diet of pacific
white shrimp in the conditions of southern part of Iran.
http://jifro.ir/article-1-625-en.pdf
2015-09-13
531
547
Pacific white shrimp
Fish meal
Soybean meal
T.
Maghsoudloo
tirdad_m@yahoo.com
1
AUTHOR
J. G.
Marammazi
2
AUTHOR
A.
Matinfar
3
AUTHOR
M.
Kazemian
4
AUTHOR
E.
Paghe
5
AUTHOR
OTHERS_CITABLE
Isolation and characterization of microsatellite loci in the Persian sturgeon (Acipenser persicus, Borodine, 1897) and cross-species amplification in four commercial sturgeons from the Caspian Sea
In order
to have a sustainable management on Persian sturgeon as a highly commercial
species in the South Caspian Sea, we need to identify its population structure
and the level as well as its conservation status in their natural habitat. To
develop a conservation program for this all Caspian Sea' sturgeon species it requires
knowledge of its genetic diversity using reliable molecular marker to study
population genetic structure. For these purposes, an enriched library was prepared
based on a modified biotin-capture method. Approximately 1800 positive clones were
screened for microsatellites in an Acipenser
persicus genomic library. Of these 350 positively hybridizing clones were
sequenced, and 81 clones were identified as having microsatellites with
adequate flanking regions. We developed and tested 68 microsatellite primer pairs
for Persian sturgeon. Out of 68 primer pairs developed, 11 pairs resulted in
poor or no amplification, 13 were ambiguous, 6 were monomorphic, 20 were
tetrasomic and 18 were octosomic in Persian sturgeon. While none of the markers
showed disomic inheritance in Persian sturgeon and Russian sturgeon (A. gueldenstaedtii).
Several of the markers appeared useful for studies stellate sturgeon (A.
stellatus), ship sturgeon (A.nudiventris) and beluga (Huso huso). Nearly
all the polymorphic pattern for ship, stellate and beluga displayed the simple
banding patterns characteristic of disomic loci, while those for Russian
sturgeon displayed banding patterns characteristic of tetraploid or higher
polyploid levels. These markers may prove useful in a variety
of future sturgeon population genetic studies in the Caspian
Sea.
http://jifro.ir/article-1-626-en.pdf
2015-09-13
548
558
Persian sturgeon
Acipenser persicus
Caspian Sea
Microsatellite
Population genetic
M.
Moghim
mehdi.moghim@gmail.com
1
AUTHOR
E. J.
Heist
2
AUTHOR
S. G.
Tan
3
AUTHOR
M.
Pourkazemi
4
AUTHOR
S. S.
Siraj
5
AUTHOR
J. M.
Panandam
6
AUTHOR
R.
Pourgholam
7
AUTHOR
D.
Kor
8
AUTHOR
F.
Laloei
9
AUTHOR
M. J.
Taghavi
10
AUTHOR
OTHERS_CITABLE
Significant changes in pelagic fish stocks of Clupeonella spp. in the south Caspian Sea
The present study was carried out
during 2006-2007 along the southern Caspian Sea. The species composition showed that the common kilka, Clupeonella cultriventris caspia, is
dominant species with 89% and then anchovy, C. engrauliformis, has shown only 8.7% while it was already dominant
species in the past decades. The overall results indicated that common kilka was
replaced with anchovy kilka as previous dominant kilka species due to invasion
of Mnemiopsis leidyi to the southern Caspian Sea. Anchovy suffered greatly from M. leidyi so
that the total catch dropped from 95000 mt in 1999 to less than 20000 mt in 2007. In our survey, 1415 fish
specimens were collected randomly, including 221 anchovy, 281 bigeye and 913
common kilka. The mean length of anchovy was 117.8 ± 0.27 mm, while it was
about 93.0 mm during 1995 to 1998 before the invasion of M. leidyi. Analysis
of age classes indicated the mean age of 4.5 ± 0.04 years for anchovy, 3.6 ±
0.03 for common kilka and 4.6 ± 0.08 for big eye C. grimmi. According to the age and the length structure of the specimens in this
study, it could be concluded that in recent years, young fish population of
anchovy has been decreased in comparison with the last decade. The results
showed that some biological characteristics of kilka population were affected
by M. leidyi. Recently
fishing activities take place in shallow waters where schools of kilka are in
suitable density for fishing. According to our results,
ctenophore occupied the anchovy habitat where it was mainly more than 50 m
depth. The fishing has moved to areas with depths less
than 50 meters where it was the main reason to change in species composition.
http://jifro.ir/article-1-628-en.pdf
2015-09-13
559
569
Age structure
Caspian Sea
Clupeonella
Species composition
Mnemiopsis leidyi
F.
Parafkandeh Haghighi
Parafkandeh@hotmail.com
1
AUTHOR
F.
Kaymaram
2
AUTHOR
OTHERS_CITABLE
Synopsis of the Parasites in Iranian Freshwater Fishes
Two hundred forty seven species of parasites from Iranian
freshwater fishes are presented in this synopsis. The parasites were recorded
from infestations in fish from different parts of the country and summarized according
to host species, organs were the parasite infestations occurred, province,
faunal region and reference numbers. The following aspects of parasite infestations
were also discussed: records of the most predominant parasites, the richest regions
of parasite fauna, records of parasites according to province and the most severely
infected fish species and finally host specificity in terms of exotic or endemic
fish.
http://jifro.ir/article-1-629-en.pdf
2015-09-13
570
589
Parasites
Freshwater
fish
Iran
J.
Pazooki
pazooki2001@yahoo.com
1
AUTHOR
M.
Masoumian
2
AUTHOR
OTHERS_CITABLE
Early development of the Amur sleeper (Perccottus glenii, Dybowski, 1877): a remarkable invasive species in Eurasia
To investigate the ontogeny of Perccottus glenii, embryonic, larval and juvenile development of P. glenii were examined under captive
condition. The fertilized eggs with numerous oil droplets were orange-pink in
color, prolate spherical in shape and had average length of 3.32±0.14 mm and width of 1.24±0.04 mm. From fertilization to hatching,
the ontogenesis of the fish spent more than 200 h, and the process of embryonic
development was divided into 25 stages based on the morphological
characteristics. The newly hatched larvae, with well-developed swim bladder and
pectoral fins, measured 5.07±0.18mm
in total length. Initial feeding occurred at day 2 after hatching (AH) and the
complete absorption of yolk sphere was observed 3 days after hatching. At day
40 AH, scales and vertical pigment were found to be appear. Scales covered the
entire body and all fins were well developed 70 days AH, at which time the
juveniles reached the young stage with a total length (TL) of 24.74±4.28 mm. At day 140 AH, The fry with a TL
of 29.57±4.65 mm, were
morphologically similar to the adults except for size.
http://jifro.ir/article-1-631-en.pdf
2015-09-13
590
601
Perccottus glenii
Early development
Invasive species
Pei-min Yang
pmyang313@163.com
1
AUTHOR
Guang-hai Jin
2
AUTHOR
Yi-xin Liu
3
AUTHOR
Jing-wei Li
4
AUTHOR
Zong-yun Hu
5
AUTHOR
OTHERS_CITABLE
Control of euglenophyte bloom and fish production enhancement using duckweed and lime
Euglenophyte
bloom is a common problem in most of the aquaculture ponds in Bangladesh. In
the present study we conducted an experiment to control euglenophyte bloom for
getting better fish production using duckweed (Lemna minor) and lime. The experiment was carried out using four
treatments, i.e., ponds were supplied with duckweed (T1), lime treatment (T2),
both duckweed and lime (T3) and without supply of duckweed and lime (T4). Rohu,
catla, mrigal, silver carp and silver barb were stocked and their gut contents were
analyzed monthly. The ranges of water quality parameters were analyzed within
the productive limit during the experimental period. The mean abundance of
euglenophyte was significantly highest in T4 (17.62 ± 1.97 × 104 cells/L), followed by T2 (2.96 ± 0.20 × 104 cells/L), T1 (1.94 ± 0.35 × 104 cells/L) and T3 (1.53 ± 0.42 × 104 cells/L). Gut content
analysis revealed that considerable amount of euglenophyte were consumed by
silver carp and silver barb, but not preferred by rohu, catla and mrigal. The
gross yields of fish were 2133.37, 1967.76, 2816.52 and 1725.62 kg/ha/5 months
in T1, T2, T3 and T4, respectively. The
highest fish production in T3 and
lowest fish production in T4 indicated the use of duckweed and
lime is economically sustainable for controlling euglenophytes bloom,
maintaining water quality and getting higher fish production.
http://jifro.ir/article-1-632-en.pdf
2015-09-13
602
617
Euglenophytes
Bloom
Duckweed
Production
Pond
Water quality
Lime
M. S.
Rahman
1
AUTHOR
M.
Shahjahan
shahjahanm75@yahoo.co.uk
2
AUTHOR
M. M.
Haque
3
AUTHOR
S.
Khan
4
AUTHOR
OTHERS_CITABLE
Antibiotic resistance pattern of some Vibrio strains isolated from seafood
The present study was aimed to evaluate the antimicrobial resistance and the presence of antibiotic resistance genes in Vibrios spp. isolated from seafood. A total of 72 isolates of Vibrio in 6 species including V. parahaemolyticus, V. vulnificus, V. alginolyticus, V. harveyi, V. mimicus and V. cholerae were examined. The results revealed that all isolates were expressing multiple antibiotic resistances. Of the 72 strains tested, 70 were resistant to ampicillin (97.2%), 60 to gentamycin (83.3%) and 56 to penicillin (77.7%). Eight strains were resistant to 4 antibiotic, 19 resistant to five antibiotics, 10 to six antibiotics, 34 to seven antibiotics and one to eight antibiotics. Results also revealed that 20 Vibrio strains (27.7% of total examined strains) contained one to three of the antibiotic resistance genes. StrB, tetS and ermB genes coding for streptomycin, tetracycline and erythromycin resistance were found in 18, 6, 5 isolates, respectively and Sulfamethoxazole resistance gene, sul2, was not detected in this study. Detection of resistance genes in Vibrio strains obtained from seafood is considered as a potential danger for consumers and also suggests that these resistance determinants might be further disseminated in habitats, thus constituting a serious health risks to human.
http://jifro.ir/article-1-633-en.pdf
2012-11-13
618
626
Vibrio spp.
Antimicrobial resistance genes
Seafood
Persian Gulf
M.
Raissy
mehdi.raissy@iaushk.ac.ir
1
AUTHOR
M.
Moumeni
2
AUTHOR
M.
Ansari
3
AUTHOR
E.
Rahimi
4
AUTHOR
OTHERS_CITABLE
Morphological Variability of the Aspius aspius taeniatus (Eichwald, 1831) in the Southern Caspian Sea Basin
Traditional morphometric measurements and meristic counts were used to
investigate the hypothesis of population fragmentation of Mash mahi,
Aspius aspius taeniatus (Eichwald, 1831) among two fishing areas in southern Caspian Sea basin(Tonekabon:32
specimens and Sari:34 specimens ).
Univariate analysis of variance showed significant differences between the
means of the two groups for 12 out of 26
standardized morphometric measurement and three out of nine meristic counts. In
discriminant function analysis (DFA), the
proportion of individuals correctly classified into their original groups was
82.1% and 61.2% for morphometric and meristic characteristics, respectively.
Clustering based on Euclidean distances among groups of centroids using an
UPGMA and also principal component analysis’ results (PCA) for morphometric and
meristic data indicated that two samples of Mash mahi were distinct from
each other in these regions, while there were a relatively high degree of
overlap between two locations.
http://jifro.ir/article-1-634-en.pdf
2015-09-13
627
643
Aspius aspius taeniatus
Morphometric
Meristic
Caspian Sea
population differentiation.
E.
Rezaei
e_re205@yahoo.com
1
AUTHOR
S.
Vatandoust
2
AUTHOR
M.
kazemian
3
AUTHOR
H.
Anvarifar
4
AUTHOR
OTHERS_CITABLE
Experimentally pathogenesis of Aeromonas hydrophila in freshwater Crayfish (Astacus leptodactylus) in iran
In this research the affect of A. hydrophila on pathogenesis of Crayfish
A. leptodactylus was carried out.
This study was designed in three groups as treatments and one group as
control in triplicate with 20 Crayfish in each glass aquarium. The treatments have exposed to A. hydrophila.
The concentrations of the bacteria in treatments were
, and
CFUmL-1 respectively.
The untreated control group was disinfected by oxytetracyclin at concentration of
100 ppm for 24 hours. The haemolymph samples were withdrawn for measuring of
THC and TPC within interval hours (2, 6, 12, 24, 48, 96, 144, 240 and 336). The
Crayfish samples were then fixed in Davidson fixative. The results showed that
the differences of THC value between treatment 4 (95.33±23.16) and control
(1.13±.45) were significant (P<0.05) as well as control and
treatments 3 (35.33±16.16) and 4 (95.33±23.16) during 2 initial hours. In case
of THC the significant difference (P<0.05) was observed between
control group (72.33±15.04) and treatment 3 (13.13±5.85) after 48 hours. During
240 hours, also the difference of THC value between control group (80.33±24.94)
with treatment 2 (14.67±3.51) was significant (P<0.05). The finding
of TPP value showed that there was no significant difference between control
group and treatments in during mentioned times (P<0.05). The result
of histopathology in heaptopancreas and gill sections showed that hemocyte
aggregation and necrosis within pyknosis of nucleus. In lower concentrations of
bacteria no pathological changes of heart were observed. In digestive tract no
changes were appeared in treatments, but in concentration of 3×108 CFU ml-1 a low aggregation of hemocytes was
revealed.
http://jifro.ir/article-1-635-en.pdf
2015-09-13
644
656
Astacus leptodactylus
Aeromonas hydrophilia
Total Hemocyte Count
Total Protein Concentration
Histopathology
A.
SamCookiyaei
Drsam.arash@gmail.com
1
AUTHOR
M.
Afsharnasab
2
AUTHOR
V.
Razavilar
3
AUTHOR
A. A.
Motalebi
4
AUTHOR
S.
Kakoolaki
5
AUTHOR
Y.
Asadpor
6
AUTHOR
M. Y.
Yahyazade
7
AUTHOR
A.
Nekuie Fard
8
AUTHOR
OTHERS_CITABLE
Ultrastructure of the chorion and the micropyle of an endemic cyprinid fish, Cyprinion tenuiradius Heckel, 1849 (Teleostei: Cyprinidae) from southern Iran
The scanning electron microscope was used to investigate
the ultrastructures of the egg membrane surface (unfertilized egg) of an
endemic cyprinid fish, Cyprinion tenuiradius Heckel, 1849. The eggs of this species were almost circular in shape, had a smooth surface and one
type II micropyle consisting
of the flat pit and a long canal in
the animal polar region. The
micropyle region was not flat, micropyle was circular or oval in shape and the
micropyle canal was located in its center. Round or oval accessory pores were
also observed in the pit around of the micropylar canal. The surface of zona
radiata was smooth with a uniform distribution of almost round pores with lips.
http://jifro.ir/article-1-636-en.pdf
2015-09-13
657
665
Morphology
Fertilization
Cyprinion tenuiradius
Micropyle
Accessory openings
H. R.
Esmaeili
esmaeili@susc.ac.ir
1
AUTHOR
A.
Gholamifard
2
AUTHOR
OTHERS_CITABLE
Antioxidant effect of ascorbic acid on the quality of Cobia (Rachycentron canadum) fillets during frozen storage
The
effect of aqueous solutions of ascorbic acid (AA) on the rancidity development
in Cobia (Rachycentron canadum) fillets during frozen storage was
studied. Cobia fillets were treated with ascorbic acid (AA 0.25% and AA 0.5%)
then stored at -18°C up to 6 months. Rancidity
development was measured by several biochemical indices including free fatty
acids (FFA), peroxide value (PV), and thiobarbituric acid (TBA) and complemented by the sensory analysis ( odor, consistency and appearance). In addition, pH and
expressible moisture (EM) were measured during 6 months storage. Proximate composition
was also determined in the first day. TBA, PV and
FFA levels increased on all treatments due to lipid oxidation. Ascorbic acid
showed antioxidative effect on Cobia fillets during frozen storage as indicated
by TBA, PV and FFA levels. Results showed that free fatty acid, primary
and secondary oxidation products, EM and
pH value of AA- treated samples were significantly lower than those of the
control samples (P<0.05). A gradual decrease (P<0.05)
in sensory analysis were observed as the storage
time increased. Results of our investigation revealed that ascorbic acid
retarded oxidative changes in frozen Cobia fillets whereas AA 0.25% was not as
effective as AA 0.5% on oxidative stability. Best oxidation inhibition results
on fish fillets were obtained when employing a 0.5% AA solution.
http://jifro.ir/article-1-637-en.pdf
2015-09-13
666
680
Cobia
Lipid oxidation
Ascorbic acid
Frozen storage
S.
Taheri
shahrooztaheri@yahoo.com
1
AUTHOR
A. A.
Motalebi
2
AUTHOR
A.
Fazlara
3
AUTHOR
OTHERS_CITABLE
Reproduction biology of chub living in Lake Hafik
In this study, the
reproductive biology of chub population in Hafik Lake was investigated. 242
samples were caught between April and November 2009. The age of S. cephalus
samples were found to range between I-VIII in addition, 153 of them were
female and 89 were male. Their fork lengths changed between 114-332 mm, and body weights
were between 22.5 and 550.3
g. Male specimens were determined to reach sexual
maturity at II and III age, while females reached at III and IV years of age.
The smallest female individual at sexual maturity had 114 mm of fork length and 27.3 g, while the smallest
male at sexual maturity had 118
mm of fork length and 24.2 g. The mean
gonadosomatic index of male and female specimens were found highest in April (12.33, 8.02) and May (8.37, 4.64)
and minimum in August (2.39, 1.22). Egg diameters of chub individuals were
measured between 343 µm and 1900 µm. The egg number in per one gram ovary was
maximum in August, and minimum in April and May. S. cephalus was
determined to lay eggs in Hafik Lake towards the end of April, which continued
until the end of July. Maximum fecundity in population was observed as 151302 in the specimen at
the age of VI.
http://jifro.ir/article-1-638-en.pdf
2015-09-13
681
692
Chub
gonad development
Hafik Lake
B.
Unver
bunver@cumhuriyet.edu.tr
1
AUTHOR
s.
Kekilli
2
AUTHOR
OTHERS_CITABLE
Annual cycle of ovarian development and sex hormones of grey mullet (Mugil cephalus) in captivity
The
grey mullet fingerling were imported to Iran in 1997 from Hong Kong and in
coastal fish pond of north part of Iran were successfully cultured in order to
obtain broodstocks and induce artificial reproduction. Seasonal changes in
serum concentrations of Testosterone, 17-β Estradiole (E2), 17-α
Hydroxy Progesterone and also the level of calcium, Triglyceride and
cholesterol in females grey mullet in captivity were measured by
Radioimmunoassay or colorimetry, respectively. Investigating of sex steroids in different
sexual maturity indicated that increasing of oocyte diameter and sexual
maturity development serum testosterone was increased significantly
(P<0.05). Although Biochemical parameters concentrations in blood serum of
grey mullet diminished significantly until third stage of sexual maturity
(P< 0.05), their level showed a rising trend at the fourth stage. A
significant increase was observed in the serum protein and cholesterol
concentrations (P 0.05). These results suggest that the seasonal changes in serum lipids and
gonadal steroids were associated with reproductive development. The data also
support the hypothesis that the shortening photoperiod is a major factor in
stimulating reproductive activity in striped mullet.
http://jifro.ir/article-1-639-en.pdf
2015-09-13
693
703
Mugil cephalus
Ovarian development
Sexual steroid hormones
Captivity
S.
Yelghi
Saeed393@yahoo.com
1
AUTHOR
S. A.
Shirangi
2
AUTHOR
R.
Ghorbani
3
AUTHOR
H. A.
Khoshbavar Rostami
4
AUTHOR
OTHERS_CITABLE
Effects of frying by different frying oils on fatty acid profile of silver carp (Hypophthalmichthys molitrix)
The study aims to determine the influence of frying (shallow
and deep) with olive, canola and sunflower oil on fatty acid composition of
silver carp. Frying by olive oil and
canola oil increased the monounsaturated fatty acids (MUFA) significantly
(p<0.05) that consequently decreased saturated fatty acids (SFA),
polyunsaturated fatty acids (PUFA) and ω-3 fatty acids. Frying by sunflower oil
increased PUFA significantly (p<0.05), which caused to decrease in SFA and
MUFA. The ω-6/ω-3 ratio of control samples (0.224) after shallow fat frying and
deep frying, increased to 1.287 and 0.615 to 2.290 and 1.538 and to 9.381 and
5.950 by olive oil, canola oil and sunflower oil, respectively. Results suggest
that frying oil can change the fatty acid composition of fish. The changes are
depending to the kind of frying oil and method of frying which used.
http://jifro.ir/article-1-640-en.pdf
2015-09-13
704
712
Frying
Fatty acid composition
ω-6/ω-3 ratio
Silver carp
E.
Zakipour Rahimabadi
e_zakipour@yahoo.com
1
AUTHOR
S.
Dad
2
AUTHOR