en Biology & physiology Biology & physiology پژوهشي Orginal research papers <span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">Vitellogenic stage carp ovaries were collected aseptically and the cells of ovarian follicles were dispersed by trypsin treatment and cultivated as monolayer. Control cultures were grown in medium M199 alone. Media for experimental cultures were supplemented either with</span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: AR-SA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US"> 100 µl ml^-1 </span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">of CPH and CPS-carp GnRH (cultivated pituitary cell secretion affected by </span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: AR-SA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">10^-6, 10^-8,10^-10 M</span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">carp GnRH)</span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: AR-SA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">.<b> </b></span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">The direct effect of these factors on </span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: AR-SA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">17-α-Hydroxy progesterone (17α -<a name="OLE_LINK6"></a><a name="OLE_LINK5"><span style="mso-bookmark: OLE_LINK6"><font face="Times New Roman" size="3">OHP</font></span></a>) and 17β-Estradiol (E₂)</span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US"> secretion of isolated follicular cells in cell culture was assayed by appropriate Radioimmunoassay (RIA). According to the results the E2 was a main hormone secreted by cultivated carp granulosa and theca cells. Adding 100 </span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: AR-SA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">µl ml^-1</span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">of </span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: AR-SA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">10^-6M</span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US"> CPS-carp GnRH had no significant effect on cultivated granulosa and theca cells endocrine activity (P> 0.05). On the other hand, carp granulosa and theca cells treated with the lower contents of CPS-carp GnRH (</span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: AR-SA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">10^-8,10 ^-10 M</span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">) showed a 4.02 and 3.74-fold increase in E₂ concentration and 4.27 and 3.64-fold increase in </span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: AR-SA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">17α -OHP</span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US"> level, which was significantly higher than CPH <a name="OLE_LINK2"></a><a name="OLE_LINK1"><span style="mso-bookmark: OLE_LINK2"><font face="Times New Roman" size="3">(P< 0.05)</font></span></a>. Also, there was a significant increase of steroid secretion in group stimulated with 100 </span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: AR-SA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US">µl ml^-1</span><span style="font-family: "Times New Roman","serif" font-size: 12pt mso-bidi-language: FA mso-fareast-font-family: "Times New Roman" mso-ansi-language: EN-US mso-fareast-language: EN-US"> of CPS and CPH, but there were no significant differences between them (P> 0.05). </span> Cyprinus carpio, Cell culture, carp GnRH, Gonadotropin hormones, Steroid hormones 394 404 http://jifro.ir/browse.php?a_code=A-10-1-165&slc_lang=en&sid=1 N. Salamat salamatnegin@yahoo.com 10031947532846003231 10031947532846003231 Yes N. Erfani Majd 10031947532846003232 10031947532846003232 No H. Hashemitabar 10031947532846003233 10031947532846003233 No M. Mesbah 10031947532846003234 10031947532846003234 No A. AhangarPour 10031947532846003235 10031947532846003235 No