en Genetics Genetics پژوهشي Orginal research papers <font face="Times New Roman" size="3"> </font><p class="MsoNormal" style="margin: 0cm 0cm 0pt text-align: justify line-height: 120%"><span style="line-height: 120% font-family: "Times New Roman","serif" font-size: 12pt">Population genetic structure of <i>Vimba vimba persa </i>was investigated using microsatellite markers from 40 regions along the Iranian coastline of the Southern Caspian Sea (Anzali lagoon and Havigh River in Gilan province, BabolRoud River in Mazandaran province and GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by the Phenol-Chlorophorm method and PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 13 loci were amplified, in which 10 of them were amplified with reasonable polymorphism and 3 were monomorphism. A total of 302 alleles were identified on average 7.55. Observed and expected heterozygosity averages were 0.80 and 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p≤0.01). The estimation of <i>F</i>st (p≤0.01) </span><span style="line-height: 120% font-family: "Times New Roman","serif" font-size: 12pt mso-fareast-font-family: "Times New Roman"">revealed significant population structuring and an estimation of the four population of <i>Vimba vimba persa </i>was identified in the Caspian Sea</span><span style="line-height: 120% font-family: "Times New Roman","serif" font-size: 12pt"> in which restocking of these species should be considered. <o:p /></span></p><font face="Times New Roman" size="3"> </font> Vimba vimba persa, Population genetic, Microsatellite, Caspian Sea, Iran 347 357 http://jifro.ir/browse.php?a_code=A-10-1-161&slc_lang=en&sid=1 S. Mohamadian 10031947532846003209 10031947532846003209 Yes S. Rezavani Gilkolaei 10031947532846003210 10031947532846003210 No S. Rouhollahi 10031947532846003211 10031947532846003211 No J. Taghavi M. 10031947532846003212 10031947532846003212 No M. Nayerani 10031947532846003213 10031947532846003213 No E. Shirzad 10031947532846003214 10031947532846003214 No A. Taheri Mirghaed 10031947532846003215 10031947532846003215 No