Iranian Journal of Fisheries Sciences

en Research Article: Studies on the growth performance, physiological and biochemical indicators and intestinal flora of largemouth bass (Micropterus salmoides) in different growth stages aquaculture aquaculture پژوهشي Orginal research papers This paper studies the differential changes in growth performance, physiological and biochemical indicators, and intestinal flora of Largemouth Bass in different growth stages, and explores and explains the reasons for the differences, so that breeders can take effective measures to improve the breeding efficiency in the necessary growth stages, and add a complete fish production file to the local and similar areas. The aquaculture pond was regularly monitored and sampled for about five months, with a total of 153 days and an interval of 30 days. After sampling the fish tissue in the test base, put the sample into a foam box with ice bags, and take it back to the laboratory to measure the changes in growth indicators, liver function indicators, antioxidant indicators, and Digestive enzyme indicators of Largemouth Bass in each group according to the national monitoring standards. In addition, the high-throughput sequencing method was used for the intestinal microorganisms of each group, and 16s rRNA analysis was carried out. During the breeding process of Largemouth Bass, the average range of weight gain rate is 35.33-71.33 (p<0.05), of hepatosmatic index is 1.61-2.71 (p<0.05), of total bilirubin is 1.00-4.12 (p<0.05), of cereal grass Transaminase is 3.24-9.53 (p<0.05), of Alanine transaminase is 10.45-38.80 (p<0.05), of Superoxide dismutase is 2.02-3.14 (p<0.05), of Catalase is 4.36-6.91 (p<0.05), and of Pepsin is 52.92-81.84 (p<0.05). The most obvious is that Largemouth Bass has a rapid growth period from June to July. In August, when parasites are widespread, the antioxidant defense system in the body of the Bass is most vulnerable to damage. In October, when the climate gradually cools, the Bass begins to accumulate fat. In addition, among the intestinal flora, the dominant bacteria indifferent growth stages are mainly Mycoplasma and Cetobacterium, and the difference in intestinal flora in different stages is obvious. With the change in growth months, there are obvious differences in the growth performance, physiology and biochemistry, and intestinal flora of Largemouth Bass in different growth stages. In the necessary stages of the breeding process, measures should be taken to improve the health status of the fish, feed nutrition, and pond water quality, and some microecological reagents can be accurately used to improve breeding efficiency. This experiment provides data support for breeding technology improvement, probiotics screening, disease prevention, and intestinal microecology research of Largemouth Bass during its growth. Largemouth Bass, Freshwater ponds, Growth, Physiology, Biochemistry, Intestinal flora 1235 1261 http://jifro.ir/browse.php?a_code=A-10-4604-1&slc_lang=en&sid=1 J. Qiu 2766587014@qq.com 100319475328460057365 100319475328460057365 Yes 13771680593 C. Zhang ccxnxy@163.com 100319475328460057366 100319475328460057366 No Tianjin Key Laboratory of Aquatic Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University School, Tianjin,300384, China Z. Lv 100319475328460057367 100319475328460057367 No Tianjin Key Laboratory of Aquatic Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University School, Tianjin,300384, China Z. Zhang 100319475328460057368 100319475328460057368 No Tianjin Key Laboratory of Aquatic Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University School, Tianjin,300384, China Y. Chu 100319475328460057369 100319475328460057369 No Tianjin Key Laboratory of Aquatic Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University School, Tianjin,300384, China D. Shang 100319475328460057370 100319475328460057370 No Tianjin Jiahe Tianyuan Ornamental Fish Culture Co., Ltd., Tianjin, 301823, China Y. Chen 100319475328460057371 100319475328460057371 No Tianjin Jiahe Tianyuan Ornamental Fish Culture Co., Ltd., Tianjin, 301823, China C. Chen 100319475328460057372 100319475328460057372 No Tianjin Key Laboratory of Aquatic Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University School, Tianjin,300384, China