en Biotechnology Biotechnology پژوهشي Orginal research papers <span style="font-size:11pt"><span style="line-height:120%"><span style="font-family:Calibri,sans-serif"><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">In the current study, the combination of ultrasound and microwave methods (UM) has been used for the extraction of fucoidan from </span></span></span></span><i><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Nizamuddinia zanardinii </span></span></span></span></i><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">and the effect of this method on the chemical and monosaccharide composition, molecular weight, cytotoxic (against human cervical cancer cell (HeLa) and hepatocellular carcinoma cell (HepG₂)), and immunomodulatory activities of the recovered fucoidan have also been investigated. The fucoidan yield under UM method was 5.53%. The extracted fucoidan chemically included 45.87% carbohydrate, 10.17% protein, 27.16% sulphate, and 1.1% uronic acid. The fucose (35.65%), mannose (28.94%), galactose (26.35%), xylose (7.74%), and glucose (1.33%) were the main monosaccharides of the extracted fucoidan. The average molecular weight of fucoidan was 748 kDa. The results</span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black"> also demonstrated that the extracted fucoidan had 62.41-78.08</span></span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">% and </span></span></span></span><span lang="TR" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">62.45-70.29</span></span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:120%"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black"> % cytotoxic activity for HeLa and HepG₂ cells, respectively. The nitric oxide (NO) production of RAW264.7 cells was increased with increasing the concentration of fucoidan and maximum NO production was found to be 37.79 &micro;mol at 50 &mu;g/mL. </span></span></span></span></span></span></span> Seaweeds, Nizamuddinia zanardinii, Fucoidan, Biological activities, Extraction methods 84 98 http://jifro.ir/browse.php?a_code=A-10-3438-1&slc_lang=en&sid=1 M. Alboofetileh alboofetileh@areeo.ac.ir 100319475328460046434 100319475328460046434 Yes Fish Processing Technology Research Center, Iranian Fisheries Science Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali, Iran M. Rezaei rezai_ma@modares.aac.ir 100319475328460046435 100319475328460046435 No Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran M. Tabarsa m.tabarsa@modaares.ac.ir 100319475328460046436 100319475328460046436 No Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran G. Cravotto giancarlo.craavotto@unito.it 100319475328460046437 100319475328460046437 No Department of Drug Science and Technology, University of Turin, Via P. Giuria 9, 10125 Turin, Italy